Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,081	G→C	6.2%	A434G (GCC→GGC)	trmF ←	tRNA uridine 5‑carboxymethylaminomethyl modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,081	0	G	C	6.2%	201.3 / 3.9	64	A434G (GCC→GGC)	trmF	tRNA uridine 5‑carboxymethylaminomethyl modification protein
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base C (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GATTTCAGGCGCTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTCCCGGCCAGCAG  >  NC_002947/2934‑3226                                                                                                                                                    |                                                                                                                                                  gATTTCAGGCGCTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCAAGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCCCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCgg                                                                                                                                                   >  1:72446/1‑148 (MQ=255)   tttCAGGCGCTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCAc                                                                                                                                                >  5:199652/1‑149 (MQ=255)       aGGCGCTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGa                                                                                                                                            <  1:202503/149‑1 (MQ=255)        ggCGCTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAg                                                                                                                                           >  2:45303/1‑149 (MQ=255)         gcgcTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAgg                                                                                                                                          <  6:171073/149‑1 (MQ=255)               ttCCTCACGCTCGATACCGTCGCGCTTTGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACa                                                                                                                                    <  6:276635/149‑1 (MQ=255)                     aCGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCgg                                                                                                                               <  6:199652/148‑1 (MQ=255)                     aCGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCg                                                                                                                                <  1:45303/147‑1 (MQ=255)                       gCTCGATACCGTCGCGCTTGGCTCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTAc                                                                                                                            <  5:144429/149‑1 (MQ=255)                       gCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTAc                                                                                                                            >  7:289013/1‑149 (MQ=255)                       gCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTAc                                                                                                                            <  7:200870/149‑1 (MQ=255)                         tCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACgg                                                                                                                          >  7:26382/1‑149 (MQ=255)                            aTACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTc                                                                                                                       <  6:158207/149‑1 (MQ=255)                             tACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCTGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTcc                                                                                                                      <  4:244567/149‑1 (MQ=255)                             tACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTcc                                                                                                                      >  7:230669/1‑149 (MQ=255)                                      gcTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTAccc                                                                                                             <  4:163210/149‑1 (MQ=255)                                          ggCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAg                                                                                                          <  8:26382/148‑1 (MQ=255)                                           gcgcAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAgg                                                                                                         <  6:97596/148‑1 (MQ=255)                                                 aaTGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAg                                                                                                  >  4:2087/1‑149 (MQ=255)                                                     cGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGgtcg                                                                                              >  3:204706/1‑149 (MQ=255)                                                      ggCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAgg                                                                                                         >  5:82095/1‑137 (MQ=255)                                                      ggCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAgg                                                                                                         <  6:82095/137‑1 (MQ=255)                                                       gCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCCCGCAGTATCAGCCGGTACTCTGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGgtcgt                                                                                             >  1:290595/1‑148 (MQ=255)                                                         ccAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTcca                                                                                          <  8:289013/149‑1 (MQ=255)                                                          cAGCTCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTccac                                                                                         <  6:241282/149‑1 (MQ=255)                                                          cAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTccac                                                                                         >  5:34721/1‑149 (MQ=255)                                                           aGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTccac                                                                                         >  3:148098/1‑148 (MQ=255)                                                              gcTGGTCATCGATCAGGCCCAGTTAACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAg                                                                                      >  5:237358/1‑148 (MQ=255)                                                                  gTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGc                                                                                 <  6:237358/149‑1 (MQ=255)                                                                      tCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGAt                                                                             >  8:284172/1‑149 (MQ=255)                                                                        gATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATg                                                                            >  8:155274/1‑148 (MQ=255)                                                                         aTCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGt                                                                                                                              <  5:190783/97‑1 (MQ=255)                                                                         aTCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGt                                                                                                                              >  6:190783/1‑97 (MQ=255)                                                                              gCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGAt                                                                             >  8:286110/1‑141 (MQ=255)                                                                              gCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGAt                                                                             <  7:286110/141‑1 (MQ=255)                                                                              gCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCt                                                                     >  7:72439/1‑149 (MQ=255)                                                                              gCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCt                                                                     >  2:111176/1‑149 (MQ=255)                                                                                      cACGGGCCTTTTCGGTCAGGCCGAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCTGTTCTCGGCACTCGAGGTAAACTTGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcg                                                              <  8:265835/148‑1 (MQ=255)                                                                                      cACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcg                                                              <  7:155274/148‑1 (MQ=255)                                                                                      cACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCCGTACGTCTCCTGATTCCCCAAGGTAATAACGTCGACCACCAGTAAGCCGATGTATGCCTCGTcccg                                                              >  7:265835/1‑148 (MQ=255)                                                                                       aCGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcggc                                                            >  1:102619/1‑149 (MQ=255)                                                                                       aCGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcggc                                                            >  5:281208/1‑149 (MQ=255)                                                                                         ggCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcggcgc                                                          <  2:290595/149‑1 (MQ=255)                                                                                           cccTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcggcgcgg                                                        >  3:282374/1‑149 (MQ=255)                                                                                           cccTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCACGTCGTCCACCAGTACGCCGATGTATGCCTCGTcgcggcgcgg                                                        >  8:89244/1‑149 (MQ=255)                                                                                            cgttttCGGTCAGGCGCAGGTCGGCGTTGTCTTCTCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGAACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCggg                                                       <  2:72446/147‑1 (MQ=255)                                                                                             cTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCggg                                                       >  3:311400/1‑148 (MQ=255)                                                                                                   ggTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAg                                                 <  3:2087/148‑1 (MQ=255)                                                                                                   ggTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGc                                                >  3:27039/1‑149 (MQ=255)                                                                                                     tCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTg                                              <  8:230669/149‑1 (MQ=255)                                                                                                           gcAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACgg                                        >  7:118008/1‑149 (MQ=255)                                                                                                            cAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGCCCCGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGc                                       >  4:231238/1‑149 (MQ=255)                                                                                                            cAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGCCACGCGAGGTAAACATGCGGTACGGATCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGc                                       >  4:226978/1‑149 (MQ=255)                                                                                                             aGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGcc                                      <  8:72439/149‑1 (MQ=255)                                                                                                                         tCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGACTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGaa                           <  3:35808/148‑1 (MQ=255)                                                                                                                         tCTTCGCGAAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTTATCAGGTCTTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACAAg                                                 >  7:133260/1‑126 (MQ=255)                                                                                                                         tCTACGCGCAGTATCAGCCGGTACTCGGCACGCGATGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAg                                                 <  8:133260/126‑1 (MQ=255)                                                                                                                              gcgcAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTgcg                      <  6:281208/148‑1 (MQ=255)                                                                                                                                     aTCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTa                >  7:244120/1‑147 (MQ=255)                                                                                                                                     aTCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTa                <  8:244120/147‑1 (MQ=255)                                                                                                                                        aGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTccc           <  4:204706/149‑1 (MQ=255)                                                                                                                                              tACTCGCCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTCCCGGCcag     <  3:226978/149‑1 (MQ=255)                                                                                                                                              tACTCGCCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTCCCGGCcag     <  3:231238/149‑1 (MQ=255)                                                                                                                                               aCTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTCCCGGCcag     >  4:116058/1‑148 (MQ=255)                                                                                                                                                 tCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTAGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTCCCGGCcagcag  >  7:14220/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GATTTCAGGCGCTGTTCCTCACGCTCGATACCGTCGCGCTTGGCGCAGAATGCGGCCCAGCGCTGGTCATCGATCAGGCCCAGTTCACGGCCCTTTTCGGTCAGGCGCAGGTCGGCGTTGTCTTCGCGCAGTATCAGCCGGTACTCGGCACGCGAGGTAAACATGCGGTACGGCTCCTGAGTACCCAAGGTAATCAGGTCGTCCACCAGTACGCCGATGTATGCCTCGTCGCGGCGCGGGCACCAGCTGTCACGGCCCTGCGCCCGAAGTGCGGCGTTAGTCCCGGCCAGCAG  >  NC_002947/2934‑3226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑