Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 48,872 T→G 8.2% D168A (GAC→GCC)  czcA‑I ← cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294748,8720TG 8.2% 146.0 / 5.0 49D168A (GAC→GCC) czcA‑Ication efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base G (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

AACGCTGCGACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCTGTT  >  NC_002947/48725‑49019
                                                                                                                                                   |                                                                                                                                                   
aaCGCTGCGACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGACAAACCCGGAACGTTACAAAACTAAGGCTTGATGATCCAGTc                                                                                                                                                    >  6:112315/1‑149 (MQ=255)
    cttcgtccAGGGCATTGCGTGTCAGCTTGTATGTAGGCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGGTTGGCATAACCGGCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTgg                                                                                                                                                <  4:63986/143‑1 (MQ=255)
             ggTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTCCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGGACTGGATCatcaag                                                                                                                                       <  1:125845/149‑6 (MQ=255)
              gTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCAcgcg                                                                                                                                        >  6:54898/1‑147 (MQ=255)
              gTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCAcgcg                                                                                                                                        >  4:29520/1‑147 (MQ=255)
               tCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAg                                                                                                                                     <  5:54898/149‑1 (MQ=255)
                 aTTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAggt                                                                                                                                   >  3:170110/1‑149 (MQ=255)
                         tCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                           <  6:47158/149‑1 (MQ=255)
                             cTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGgtgt                                                                                                                       <  4:139134/149‑1 (MQ=255)
                                   ggtagCCAGGCGCTTGGGATCCGGCGCAACCAGGAACTGCTTTGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCg                                                                                                                 <  6:24662/149‑1 (MQ=255)
                                     tagCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTa                                                                                                               <  2:170903/149‑1 (MQ=255)
                                        ccAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCg                                                                                                            <  3:29520/149‑1 (MQ=255)
                                        ccAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGGACTGGATCatcaa                                                                                                                                        >  6:235561/1‑117 (MQ=255)
                                        ccAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGGACTGGATCatcaa                                                                                                                                        <  5:235561/121‑5 (MQ=255)
                                         cAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGt                                                                                                           >  8:40142/1‑149 (MQ=255)
                                                tttGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCttt                                                                                                    >  6:63351/1‑149 (MQ=255)
                                                  tGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCttt                                                                                                    <  2:38355/147‑1 (MQ=255)
                                                               aCCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTc                                                                                     <  4:170110/149‑1 (MQ=255)
                                                                     aaCTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCct                                                                                                        >  5:241793/1‑124 (MQ=255)
                                                                     aaCTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCct                                                                                                        <  6:241793/124‑1 (MQ=255)
                                                                      aCTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTc                                                                               >  4:138542/1‑148 (MQ=255)
                                                                          cTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAg                                                                          >  2:157122/1‑149 (MQ=255)
                                                                              gCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCca                                                                      >  5:311710/1‑149 (MQ=255)
                                                                               cATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTTCGGCGTACCGTCCTCTTTGACCGCGCCATCTTCgg                                                                                   <  1:209454/135‑1 (MQ=255)
                                                                               cATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTTCGGCGTACCGTCCTCTTTGACCGCGCCATCTTCgg                                                                                   >  2:209454/1‑135 (MQ=255)
                                                                                 tAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCttt                                                                                                    <  6:269358/116‑1 (MQ=255)
                                                                                 tAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCttt                                                                                                    >  5:269358/1‑116 (MQ=255)
                                                                                             gTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGcc                                                       <  3:138542/149‑1 (MQ=255)
                                                                                              tATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCaa                                                                             >  5:132813/1‑126 (MQ=255)
                                                                                              tATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCaa                                                                             <  6:132813/126‑1 (MQ=255)
                                                                                                 tGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAg                                                    <  7:40142/148‑1 (MQ=255)
                                                                                                        ggCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCgg                                               >  5:65303/1‑146 (MQ=255)
                                                                                                        ggCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTa                                             <  6:65303/148‑1 (MQ=255)
                                                                                                          ccACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGa                                           >  5:306198/1‑148 (MQ=255)
                                                                                                          ccACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGa                                           >  5:301540/1‑148 (MQ=255)
                                                                                                                gcGGGACGTGACGCAGCTGAGGCTTGTTGATCCAGTCCTGGATCACGCGCAGGGCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGa                                    <  8:129184/148‑1 (MQ=255)
                                                                                                                  gggACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGAcc                                  <  1:157365/149‑1 (MQ=255)
                                                                                                                   ggACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGAccc                                 <  6:311710/149‑1 (MQ=255)
                                                                                                                        ttACGAAGCTGAGGCTTGATGATCCAGTCCTGGATCACCCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTATTTGACAGCGCCATCTTCGGCTTCAACAGTACACAGGAAGATCTCGCCGAGGCCGCTAGATACAGGACCAATGAc                            >  1:300633/1‑149 (MQ=37)
                                                                                                                          aCGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACg                           <  6:301540/148‑1 (MQ=255)
                                                                                                                          aCGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACg                           <  6:306198/148‑1 (MQ=255)
                                                                                                                            gCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCATCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGcc                        >  5:71459/1‑149 (MQ=255)
                                                                                                                                  gAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTaca                  >  1:263492/1‑149 (MQ=255)
                                                                                                                                     gCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCt               >  2:136737/1‑149 (MQ=255)
                                                                                                                                         gatgatCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTc             <  1:136737/147‑1 (MQ=255)
                                                                                                                                         cgtgatCCAGGACTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTccg           >  5:259854/3‑147 (MQ=255)
                                                                                                                                          atgagCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGGACGGAGTACCGTCCGCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGTTAGATACCGGACCCATGACGGCCTCTACACCTTCTgg          <  2:265803/149‑1 (MQ=255)
                                                                                                                                                  gTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCTGtt  >  5:40993/1‑149 (MQ=255)
                                                                                                                                                  gTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCTGtt  >  8:196109/1‑149 (MQ=255)
                                                                                                                                                   tCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAg                                                               >  4:292536/1‑87 (MQ=255)
                                                                                                                                                   tCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAg                                                               <  3:292536/87‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACGCTGCGACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCTGTT  >  NC_002947/48725‑49019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑