Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,248,723	A→C	6.0%	I435S (ATC→AGC)	PP_3724 ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,248,723	0	A	C	6.0%	216.4 / 3.5	67	I435S (ATC→AGC)	PP_3724	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base C (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGT  >  NC_002947/4248576‑4248865                                                                                                                                                      |                                                                                                                                               aaCAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGAt                                                                                                                                               <  5:114488/149‑1 (MQ=255)  aCAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATg                                                                                                                                              >  2:52588/1‑149 (MQ=255)   cAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCTGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATg                                                                                                                                              <  1:52588/149‑1 (MQ=255)      tCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCGGGGCATCGCTGccct                                                                                                                                          >  5:221112/1‑148 (MQ=255)          cTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACCCCCTGCAGGGCATCGCTGCCCtgcag                                                                                                                                      <  3:123199/149‑3 (MQ=255)           tttCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCa                                                                                                                                     >  8:184892/1‑149 (MQ=255)               gCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCGGGGCATCGCTGCCCtgcagggtgt                                                                                                                                 <  6:221112/149‑8 (MQ=255)                cccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                                <  2:90385/149‑1 (MQ=255)                  cGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGgc                                                                                                                              >  4:15363/1‑149 (MQ=255)                          ccTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgc                                                                                                                      <  1:161632/149‑1 (MQ=255)                            tGCGGGTACCGCTGTGCCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgcg                                                                                                                     >  7:29900/1‑148 (MQ=255)                            tGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgc                                                                                                                      <  3:21878/147‑1 (MQ=255)                            tGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgc                                                                                                                      >  4:21878/1‑147 (MQ=255)                               gggCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGATTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCAc                                                                                                                  >  1:80544/1‑149 (MQ=255)                                ggCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTg                                                                                                                >  5:206592/1‑149 (MQ=255)                                  cacaGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTgg                                                                                                               >  1:90448/1‑148 (MQ=255)                                        ggTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCaa                                                                                                        >  1:109655/1‑149 (MQ=255)                                          tACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACa                                                                                                      <  7:245483/149‑1 (MQ=255)                                             ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCt                                                                                                                           <  4:113184/125‑1 (MQ=255)                                             ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCt                                                                                                                           >  3:113184/1‑125 (MQ=255)                                             ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGtt                                                                                                   >  7:222089/1‑149 (MQ=255)                                               gCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                                <  2:307317/118‑1 (MQ=255)                                               gCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                                >  1:307317/1‑118 (MQ=255)                                               gCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                                 >  1:263658/1‑149 (MQ=255)                                               gCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                                 <  2:90448/149‑1 (MQ=255)                                                  caGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCggg                                                                                              <  3:15363/149‑1 (MQ=255)                                                     ccTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggcag                                                                                                            <  2:300330/132‑1 (MQ=255)                                                     ccTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggcag                                                                                                            >  1:300330/1‑132 (MQ=255)                                                       tCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGc                                                                                         >  2:217379/1‑149 (MQ=255)                                                           ccTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                                     >  8:27086/1‑149 (MQ=255)                                                            cTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAgg                                                                                    <  2:109655/149‑1 (MQ=255)                                                              ggTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGt                                                                                   >  5:313922/1‑148 (MQ=255)                                                                  aaagaaCCGCCAGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACg                                                                               >  7:163607/5‑148 (MQ=255)                                                                  aaagaaCCGCCAGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCAACCCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCCCGCGCACTGGCAGGCAACAGTTCCGGGAACGCCGAGGTGCCg                                                                               >  7:159146/5‑148 (MQ=255)                                                                       aCCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAAc                                                                                                       <  1:306758/119‑1 (MQ=255)                                                                       aCCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAAc                                                                                                       >  2:306758/1‑119 (MQ=255)                                                                           cTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCt                                                                     >  2:290557/1‑149 (MQ=255)                                                                            tGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCCGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCtt                                                                    >  8:206431/1‑149 (MQ=255)                                                                                        ttGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGc                                                            <  2:311615/146‑1 (MQ=255)                                                                                        ttGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGc                                                            <  2:263658/146‑1 (MQ=255)                                                                                         tGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCAGACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGtt                                                         <  7:189688/148‑1 (MQ=255)                                                                                            tAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAAc                                                     >  7:187601/1‑149 (MQ=255)                                                                                               gCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACgg                                                   >  4:283677/1‑148 (MQ=255)                                                                                                  acgaTGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATc                                               >  2:79699/1‑149 (MQ=255)                                                                                                   cgaTGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCg                                              <  7:184892/149‑1 (MQ=255)                                                                                                       gcgcCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACAGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAg                                           >  3:246067/1‑148 (MQ=255)                                                                                                         gcCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTa                                         >  7:76471/1‑148 (MQ=255)                                                                                                           ccccAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCtt                                                                    <  4:58512/119‑1 (MQ=255)                                                                                                           ccccAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCtt                                                                    >  3:58512/1‑119 (MQ=255)                                                                                                            cccAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGc                                     <  7:27086/149‑1 (MQ=255)                                                                                                                   c‑gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcag                                >  3:282797/1‑147 (MQ=255)                                                                                                                     gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcagcag                             <  1:79699/149‑1 (MQ=255)                                                                                                                      ccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcagcagc                            <  8:222089/149‑1 (MQ=255)                                                                                                                           ccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTc                       <  8:29900/149‑1 (MQ=255)                                                                                                                              tCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCa                    >  2:290379/1‑149 (MQ=255)                                                                                                                              tCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCa                    <  4:282797/149‑1 (MQ=255)                                                                                                                                  ccACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCAt                   <  6:206592/146‑1 (MQ=255)                                                                                                                                   cacaCCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCt               >  7:235502/1‑149 (MQ=255)                                                                                                                                      acCCTGCCGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGc            <  8:163607/149‑1 (MQ=255)                                                                                                                                      acCCTGCAGTGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGTGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCCGTACAGCCCCAGCAGCATGTCGCATTCCTTGc            <  8:159146/149‑1 (MQ=255)                                                                                                                                        ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAg                                                                            <  7:230970/83‑1 (MQ=255)                                                                                                                                        ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAg                                                                            >  8:230970/1‑83 (MQ=255)                                                                                                                                        ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGt          <  3:283677/149‑1 (MQ=255)                                                                                                                                           gcggggCATCGCTGCCCTGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCg       >  7:76274/4‑149 (MQ=255)                                                                                                                                             aGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGcc     <  1:290557/149‑1 (MQ=255)                                                                                                                                              gggCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCg    <  6:313922/149‑1 (MQ=255)                                                                                                                                                gCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACCGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGt  <  4:246067/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                               AACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGG‑TTCTTTTGGTAAGCGACGATGCGCCCCCAGGCC‑GCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGT  >  NC_002947/4248576‑4248865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑