Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,252,205 G→C 13.2% intergenic (‑173/+67) PP_3726 ← / ← rocE enoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,252,2050GC13.2% 140.8 / 13.1 53intergenic (‑173/+67)PP_3726/rocEenoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE
Reads supporting (aligned to +/- strand):  ref base G (28/18);  new base C (5/2);  total (33/20)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

AGGACTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAG  >  NC_002947/4252065‑4252341
                                                                                                                                             |                                                                                                                                        
aggaCTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCt                                                                                                                                  >  7:258045/1‑149 (MQ=255)
       gatgaGGGAGATACTAAACGAGTGTTCAGAAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGAACCGCGTAGGCGGCGCTCGACCt                                                                                                                            >  5:160813/1‑148 (MQ=255)
          gaGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTca                                                                                                                          >  5:189732/1‑147 (MQ=38)
           aGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATCGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTcaca                                                                                                                        <  5:295388/148‑1 (MQ=37)
            gggAGATCCGAAACGAGTGTTCAGCAAGGCGGGATGGTCTGTTACAACTTCGCACAACTCGGTGTCTGTCTTTACACAAGGGCG‑TTCGTCATGACGGCTTTAGGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAgg                                                                                                                      <  7:209263/149‑1 (MQ=25)
                 aTACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                              <  5:89088/136‑1 (MQ=255)
                 aTACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                              >  6:89088/1‑136 (MQ=255)
                   aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCt                                                                                                                            <  1:197378/136‑1 (MQ=255)
                   aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCt                                                                                                                            >  2:197378/1‑136 (MQ=255)
                   aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGa                                                                                                                >  6:265180/1‑148 (MQ=255)
                    cTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaa                                                                                                              <  5:265180/149‑1 (MQ=255)
                     tAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaaa                                                                                                             >  2:247481/1‑149 (MQ=255)
                      aaaCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAAt                                                                                                            >  7:24798/1‑149 (MQ=255)
                        aCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGc                                                                                                          <  3:309915/149‑1 (MQ=255)
                         cGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCa                                                                                                         <  1:247481/149‑1 (MQ=255)
                               ttCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                    >  5:333686/1‑148 (MQ=255)
                               ttCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                    >  6:47436/1‑148 (MQ=255)
                                    cAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAaccc                                                                                              >  5:82970/1‑147 (MQ=255)
                                        gCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaaa                                                                                                             >  1:35588/1‑130 (MQ=255)
                                        gCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaaa                                                                                                             <  2:35588/130‑1 (MQ=255)
                                                       aCAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGAGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcg                                                                           >  5:139667/1‑149 (MQ=255)
                                                        cAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcgg                                                                          >  3:109116/1‑149 (MQ=255)
                                                         aaCTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcggc                                                                         >  7:302784/1‑149 (MQ=255)
                                                              cGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTc                                                                      >  1:206550/1‑148 (MQ=255)
                                                               gCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCaaa                                                                   <  6:160813/149‑1 (MQ=255)
                                                                cacaACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAc                                                                  <  6:333686/149‑1 (MQ=255)
                                                                cacaACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAc                                                                  >  7:57428/1‑149 (MQ=255)
                                                                     cTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                             >  4:62428/1‑149 (MQ=255)
                                                                     cTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                             <  5:163183/149‑1 (MQ=255)
                                                                     cTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                             >  2:32142/1‑149 (MQ=255)
                                                                        ggTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGgt                                                           <  8:24798/148‑1 (MQ=255)
                                                                             cTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCt                                                      <  8:302784/148‑1 (MQ=255)
                                                                              tGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTgg                                                    >  3:117968/1‑149 (MQ=255)
                                                                                  gTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGc                                                >  4:42918/1‑149 (MQ=255)
                                                                                     acacAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCg                                               >  2:197719/1‑147 (MQ=255)
                                                                                       acaAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAgg                                           >  6:107183/1‑149 (MQ=255)
                                                                                         aaGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGcc                                         >  3:123939/1‑149 (MQ=255)
                                                                                             gCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCt                                     <  6:139667/149‑1 (MQ=255)
                                                                                               g‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGc                                   >  2:302761/1‑149 (MQ=255)
                                                                                                    gTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGa                                <  2:206550/148‑1 (MQ=255)
                                                                                                       aTGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcggc                            >  3:129743/1‑149 (MQ=255)
                                                                                                          aCGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcggcgg                          <  8:258045/148‑1 (MQ=255)
                                                                                                                     gCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACgaagaa               <  4:123939/148‑1 (MQ=255)
                                                                                                                       cTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACCCCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTa            >  3:257115/1‑149 (MQ=37)
                                                                                                                            aggtCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGa                      >  2:209278/4‑134 (MQ=37)
                                                                                                                             gaTCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTAcgccg       >  3:21421/1‑148 (MQ=255)
                                                                                                                                cgcgCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa   >  2:7159/1‑149 (MQ=255)
                                                                                                                                cgagcgccGCCTACGCGGTGCGCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCa     >  8:140110/7‑147 (MQ=255)
                                                                                                                                 gcgcACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa   <  1:32142/148‑1 (MQ=255)
                                                                                                                                 gcgcACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAg  <  1:197719/149‑1 (MQ=255)
                                                                                                                                  agcgccGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAg  >  3:124411/5‑148 (MQ=255)
                                                                                                                                            aGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa   <  6:261294/137‑1 (MQ=255)
                                                                                                                                            aGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa   >  5:261294/1‑137 (MQ=255)
                                                                                                                                             |                                                                                                                                        
AGGACTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAG  >  NC_002947/4252065‑4252341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑