Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,252,217	C→T	10.3%	intergenic (‑185/+55)	PP_3726 ← / ← rocE	enoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,252,217	0	C	T	10.3%	158.2 / 7.4	58	intergenic (‑185/+55)	PP_3726/rocE	enoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE
Reads supporting (aligned to +/- strand):  ref base C (31/21);  new base T (4/2);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGCCAAAGCCC  >  NC_002947/4252072‑4252365                                                                                                                                                   |                                                                                                                                                     gatgaGGGAGATACTAAACGAGTGTTCAGAAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGAACCGCGTAGGCGGCGCTCGACCt                                                                                                                                                    >  5:160813/1‑148 (MQ=255)    gaGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTca                                                                                                                                                  >  5:189732/1‑147 (MQ=38)     aGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATCGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTcaca                                                                                                                                                <  5:295388/148‑1 (MQ=37)      gggAGATCCGAAACGAGTGTTCAGCAAGGCGGGATGGTCTGTTACAACTTCGCACAACTCGGTGTCTGTCTTTACACAAGGGCG‑TTCGTCATGACGGCTTTAGGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAgg                                                                                                                                              <  7:209263/149‑1 (MQ=25)           aTACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                                                      <  5:89088/136‑1 (MQ=255)           aTACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                                                      >  6:89088/1‑136 (MQ=255)             aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCt                                                                                                                                                    <  1:197378/136‑1 (MQ=255)             aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCt                                                                                                                                                    >  2:197378/1‑136 (MQ=255)             aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGa                                                                                                                                        >  6:265180/1‑148 (MQ=255)              cTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaa                                                                                                                                      <  5:265180/149‑1 (MQ=255)               tAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaaa                                                                                                                                     >  2:247481/1‑149 (MQ=255)                aaaCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAAt                                                                                                                                    >  7:24798/1‑149 (MQ=255)                  aCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGc                                                                                                                                  <  3:309915/149‑1 (MQ=255)                   cGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCa                                                                                                                                 <  1:247481/149‑1 (MQ=255)                         ttCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                                            >  6:47436/1‑148 (MQ=255)                         ttCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                                            >  5:333686/1‑148 (MQ=255)                              cAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAaccc                                                                                                                      >  5:82970/1‑147 (MQ=255)                                  gCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaaa                                                                                                                                     <  2:35588/130‑1 (MQ=255)                                  gCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGaaaa                                                                                                                                     >  1:35588/1‑130 (MQ=255)                                                 aCAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGAGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcg                                                                                                   >  5:139667/1‑149 (MQ=255)                                                  cAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcgg                                                                                                  >  3:109116/1‑149 (MQ=255)                                                   aaCTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcggc                                                                                                 >  7:302784/1‑149 (MQ=255)                                                        cGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTc                                                                                              >  1:206550/1‑148 (MQ=255)                                                         gCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCaaa                                                                                           <  6:160813/149‑1 (MQ=255)                                                          cacaACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAc                                                                                          >  7:57428/1‑149 (MQ=255)                                                          cacaACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAc                                                                                          <  6:333686/149‑1 (MQ=255)                                                               cTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                                                     >  2:32142/1‑149 (MQ=255)                                                               cTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                                                     <  5:163183/149‑1 (MQ=255)                                                               cTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                                                     >  4:62428/1‑149 (MQ=255)                                                                  ggTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGgt                                                                                   <  8:24798/148‑1 (MQ=255)                                                                       cTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCt                                                                              <  8:302784/148‑1 (MQ=255)                                                                        tGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTgg                                                                            >  3:117968/1‑149 (MQ=255)                                                                            gTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGc                                                                        >  4:42918/1‑149 (MQ=255)                                                                               acacAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCg                                                                       >  2:197719/1‑147 (MQ=255)                                                                                 acaAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAgg                                                                   >  6:107183/1‑149 (MQ=255)                                                                                   aaGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGcc                                                                 >  3:123939/1‑149 (MQ=255)                                                                                       gCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCt                                                             <  6:139667/149‑1 (MQ=255)                                                                                         g‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGc                                                           >  2:302761/1‑149 (MQ=255)                                                                                              gTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGa                                                        <  2:206550/148‑1 (MQ=255)                                                                                                 aTGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcggc                                                    >  3:129743/1‑149 (MQ=255)                                                                                                    aCGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcggcgg                                                  <  8:258045/148‑1 (MQ=255)                                                                                                               gCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACgaagaa                                       <  4:123939/148‑1 (MQ=255)                                                                                                                 cTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACCCCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTa                                    >  3:257115/1‑149 (MQ=37)                                                                                                                      aggtCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGa                                              >  2:209278/4‑134 (MQ=37)                                                                                                                       gaTCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTAcgccg                               >  3:21421/1‑148 (MQ=255)                                                                                                                          cgcgCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa                           >  2:7159/1‑149 (MQ=255)                                                                                                                          cgagcgccGCCTACGCGGTGCGCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCa                             >  8:140110/7‑147 (MQ=255)                                                                                                                           gcgcACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa                           <  1:32142/148‑1 (MQ=255)                                                                                                                           gcgcACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAg                          <  1:197719/149‑1 (MQ=255)                                                                                                                            agcgccGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAg                          >  3:124411/5‑148 (MQ=255)                                                                                                                                      aGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa                           >  5:261294/1‑137 (MQ=255)                                                                                                                                      aGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa                           <  6:261294/137‑1 (MQ=255)                                                                                                                                        gcggcgCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATg              >  2:737/1‑148 (MQ=255)                                                                                                                                         cggcgCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATg              >  3:216675/1‑147 (MQ=255)                                                                                                                                         cggcgCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGaa            <  5:47436/149‑1 (MQ=255)                                                                                                                                          ggcgCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAg           >  4:273946/1‑149 (MQ=255)                                                                                                                                           gcgcTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGc          <  4:129743/149‑1 (MQ=255)                                                                                                                                           gcgcTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGc          <  3:273946/149‑1 (MQ=255)                                                                                                                                           gcgcTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGc          <  8:57428/149‑1 (MQ=255)                                                                                                                                                  aCCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGCCAAAGcc   >  3:241296/1‑149 (MQ=255)                                                                                                                                                   ccTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGCCAAAGccc  >  1:190101/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCG‑TTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAGCCAAAGCCC  >  NC_002947/4252072‑4252365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑