Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,269,462 G→T 8.5% intergenic (‑162/‑399) PP_3740 ← / → mrdA‑I MFS transporter/transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,269,4620GT 8.5% 143.4 / 2.6 47intergenic (‑162/‑399)PP_3740/mrdA‑IMFS transporter/transpeptidase
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base T (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GCCAGGCTTGGCCAAAGGCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTATAAATAAATTCAATAGTTTATAAATTCACAACCAAC  >  NC_002947/4269315‑4269609
                                                                                                                                                    |                                                                                                                                                   
gCCAGGCTTGGTCAAAGGCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCT‑GGAGGGCTCCGGCCGTTGt                                                                                                                                                    >  5:133424/1‑149 (MQ=255)
      cTTGGCCAAAGGCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGgcg                                                                                                                                              >  5:161303/1‑149 (MQ=255)
               aGGCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCT‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAAc                                                                                                                                     <  6:133424/149‑1 (MQ=255)
               aGGCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAAc                                                                                                                                     <  1:40907/149‑1 (MQ=255)
                 gCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCa                                                                                                                                   <  5:117733/149‑1 (MQ=255)
                  cGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCa                                                                                                                                   <  3:214961/148‑1 (MQ=255)
                        gAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGATGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAGCCAGATAATg                                                                                                                            >  8:80852/1‑149 (MQ=255)
                          aCGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGa                                                                                                                          >  2:69362/1‑149 (MQ=255)
                             aTGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATc                                                                                                                        >  8:88546/1‑148 (MQ=255)
                                 gcACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTTTCAGGCGCTAATCAACCAGATAATgg                                                                                                                           >  5:21622/1‑141 (MQ=255)
                                 gcACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTTTCAGGCGCTAATCAACCAGATAATgg                                                                                                                           <  6:26213/141‑1 (MQ=255)
                                 gcACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTTTCAGGCGCTAATCAACCAGATAATgg                                                                                                                           <  6:21622/141‑1 (MQ=255)
                                 gcACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTTTCAGGCGCTAATCAACCAGATAATgg                                                                                                                           >  5:26213/1‑141 (MQ=255)
                                     gcgcAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACaa                                                                                                               <  3:282967/149‑1 (MQ=255)
                                       gcAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACg                                                                                                             >  2:191667/1‑149 (MQ=255)
                                         aGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGACAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGt                                                                                                            >  6:129354/1‑148 (MQ=255)
                                                aCGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAAc                                                                                                    >  1:31220/1‑149 (MQ=255)
                                                    gaggaACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACt                                                                                                <  7:88546/149‑1 (MQ=255)
                                                       gaACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGcc                                                                                             >  2:161895/1‑149 (MQ=255)
                                                           ccTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGACAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAAc                                                                                         <  5:129354/149‑1 (MQ=255)
                                                            cTGACGGTTCAGTGCCGCTTGATAGCCATTCATGACTGGACCCTTTTTCTAATTGTTATCGCATACGGCA‑GGATGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACt                                                                                        <  5:188044/149‑1 (MQ=255)
                                                             tGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATc                                                                                                                                        >  6:150960/1‑100 (MQ=255)
                                                             tGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATc                                                                                                                                        <  5:150960/100‑1 (MQ=255)
                                                               aCGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTAt                                                                                      <  3:270118/148‑1 (MQ=255)
                                                                cGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCa                                                                                    <  6:39298/149‑1 (MQ=255)
                                                                 ggTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATa                                                                                                                               >  6:141230/1‑105 (MQ=255)
                                                                 ggTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATa                                                                                                                               <  5:141230/105‑1 (MQ=255)
                                                                 ggTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAg                                                                                   <  6:161303/149‑1 (MQ=255)
                                                                       gTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAGCCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGa                                                                             <  7:80852/149‑1 (MQ=255)
                                                                          ccGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGAcc                                                                           >  4:166032/1‑148 (MQ=255)
                                                                            gCTGGATAGCCATTCATGACTGGACACTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAAt                                                                        <  2:82335/149‑1 (MQ=255)
                                                                              tGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCAGGGAGGGATCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCATAACAATCAGGCGCGACCAAtt                                                                       >  1:245864/1‑149 (MQ=255)
                                                                                gATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAAt                                                                                                                             >  5:287516/1‑92 (MQ=255)
                                                                                gATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAAt                                                                                                                             <  6:287516/92‑1 (MQ=255)
                                                                                gATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCa                                                                    <  3:8984/149‑1 (MQ=255)
                                                                                     ccATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCTACCAATTTCAGTTCa                                                               >  7:110293/1‑149 (MQ=255)
                                                                                     ccATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCa                                                               >  5:200190/1‑149 (MQ=255)
                                                                                        ttCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGACGAGACCAATTTCAGTTCATTg                                                            >  4:191784/1‑149 (MQ=255)
                                                                                         taaTGACTGGAACCTTTTTGTAATTGTTATCGCATACGGCA‑TGAGGGCTCCGGCCGTTGTCAGGCTCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTg                                                            <  8:110293/146‑1 (MQ=255)
                                                                                                tGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTaa                                                     >  7:182458/1‑148 (MQ=255)
                                                                                                             tAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATAttttt                                        <  8:182458/148‑1 (MQ=255)
                                                                                                                   ttATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTataaa                                  >  5:33074/1‑148 (MQ=255)
                                                                                                                       cGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGACAGTAAAAATCATATTTTTTATaaataaat                             <  1:191667/149‑1 (MQ=255)
                                                                                                                          aTACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTATAAATAAATTc                           >  7:159226/1‑148 (MQ=255)
                                                                                                                           tACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATGATATTTTTTATAAATAAATTCaa                         <  6:115009/149‑1 (MQ=255)
                                                                                                                             cGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTATAAATAAATTCAAt                        <  2:31220/148‑1 (MQ=255)
                                                                                                                             cGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTATAAATAAATTCAATa                       <  2:245864/149‑1 (MQ=255)
                                                                                                                                                    gTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTATAAATAAATTCAATAGTTTATAAATTCAcaaccaac  <  6:200190/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCAGGCTTGGCCAAAGGCGAGAGGAACGATGCGCACGCGCAGGCGCAACGGGAGGAACCCTGACGGTTCAGTGCCGCTGGATAGCCATTCATGACTGGACCCTTTTTGTAATTGTTATCGCATACGGCA‑GGAGGGCTCCGGCCGTTGTCAGGCGCTAATCAACCAGATAATGGATCCACAGACAACGTGACGAAACCACTGCCTAACTATCAGGCGCGACCAATTTCAGTTCATTGCCAGTAAAAATCATATTTTTTATAAATAAATTCAATAGTTTATAAATTCACAACCAAC  >  NC_002947/4269315‑4269609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑