Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,331,476 A→T 6.7% E59V (GAA→GTA)  PP_3803 → cation ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,331,4760AT 6.7% 207.5 / 3.1 69E59V (GAA→GTA) PP_3803cation ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base T (2/3);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CCAGGACTGGGCCACTGCCGGTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCG  >  NC_002947/4331330‑4331621
                                                                                                                                                  |                                                                                                                                                 
ccAGGACTGGGCCACTGCCGGTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAg                                                                                                                                                 >  7:174565/1‑149 (MQ=255)
    gACTGGGCCACTGCCGGTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGt                                                                                                                                             >  5:323481/1‑149 (MQ=255)
         ggCCACTGCCGGTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGc                                                                                                                                         >  7:187945/1‑148 (MQ=255)
               tGCCGGTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATgcgg                                                                                                                                  >  4:89622/1‑149 (MQ=255)
                 ccGGTTAGCTGGCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGTTGGTGGGCAAGCGCTTTGCGTTCTTCTCTGTAGCGGTCGGCCATgcgg                                                                                                                                  <  2:257621/147‑1 (MQ=255)
                   ggTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCt                                                                                                                              <  5:293692/149‑1 (MQ=255)
                   ggTTATCTGCCCGAGGCGCTGGCCTACGGCTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCt                                                                                                                              >  8:256217/1‑149 (MQ=255)
                           gCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCg                                                                                                                      >  8:120768/1‑149 (MQ=255)
                           gCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCg                                                                                                                      >  4:11482/1‑149 (MQ=255)
                            cccGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGt                                                                                                                     >  1:15033/1‑149 (MQ=255)
                             ccGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAg                                                                                                                                                 >  1:142428/1‑120 (MQ=255)
                             ccGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAg                                                                                                                                                 <  2:142428/120‑1 (MQ=255)
                             ccGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTg                                                                                                                    <  3:11482/149‑1 (MQ=255)
                                   cgcTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGCTCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATc                                                                                                              >  1:246906/1‑149 (MQ=255)
                                   cgcTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCTATGCGGCGCTGACCGGCGTGGCCATc                                                                                                              <  1:166532/149‑1 (MQ=255)
                                   cgcTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATc                                                                                                              <  1:171730/149‑1 (MQ=255)
                                    gcTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGTTCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCg                                                                                                             <  2:246906/149‑1 (MQ=255)
                                          cTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATcc                                                                                                       <  7:120768/149‑1 (MQ=255)
                                          cTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATcc                                                                                                       >  3:163739/1‑149 (MQ=255)
                                              ggTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCctgct                                                                                                   <  5:61839/149‑1 (MQ=255)
                                                tttCGTGGTCAATGCCTTGCTGGCCGGCAGGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTgg                                                                                                 <  3:72074/149‑1 (MQ=255)
                                                   cGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGt                                                                                               <  8:205288/148‑1 (MQ=255)
                                                        tCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGccc                                                                                         <  7:256217/149‑1 (MQ=255)
                                                                ttGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCgg                                                                                 >  7:758/1‑149 (MQ=255)
                                                                  gCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGc                                                                                <  4:163739/148‑1 (MQ=255)
                                                                   cTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGcc                                                                               <  2:15033/148‑1 (MQ=255)
                                                                    tGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCt                                                                             >  7:289262/1‑149 (MQ=255)
                                                                    tGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCt                                                                             >  3:148446/1‑149 (MQ=255)
                                                                     caccgggcCGATCATCAGGCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCt                                                                             >  6:268619/3‑148 (MQ=255)
                                                                      gccggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTAGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTa                                                                           <  8:160021/149‑1 (MQ=255)
                                                                      gccggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTGGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTa                                                                           <  4:238324/149‑1 (MQ=255)
                                                                        cggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGt                                                                                                                                             <  6:277749/81‑1 (MQ=255)
                                                                        cggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGt                                                                                                                                             >  5:277749/1‑81 (MQ=255)
                                                                        cggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACg                                                                         >  7:17848/1‑149 (MQ=255)
                                                                        cggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACg                                                                         <  6:323481/149‑1 (MQ=255)
                                                                         ggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACgg                                                                        <  8:174565/149‑1 (MQ=255)
                                                                         ggccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACgg                                                                        <  8:84798/149‑1 (MQ=255)
                                                                           ccTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACgg                                                                        >  5:190748/1‑147 (MQ=255)
                                                                              gatgatCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACg                                                                         <  3:194102/143‑1 (MQ=255)
                                                                              gatgatCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACg                                                                         >  4:194102/1‑143 (MQ=255)
                                                                                   tCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTc                                                              <  3:89622/149‑1 (MQ=255)
                                                                                      gCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCt                                                                                                                              >  3:206855/1‑82 (MQ=255)
                                                                                      gCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCt                                                                                                                              <  4:206855/82‑1 (MQ=255)
                                                                                      gCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCgg                                                            <  2:274266/148‑1 (MQ=255)
                                                                                      gCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCgg                                                            >  1:274266/1‑148 (MQ=255)
                                                                                      gCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGc                                                           <  4:148446/149‑1 (MQ=255)
                                                                                              cTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTActgcct                                                   >  5:307026/1‑149 (MQ=255)
                                                                                                 ggTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGTAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCctgct                                                >  6:305480/1‑149 (MQ=255)
                                                                                                 ggTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGTAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCctgct                                                >  6:310052/1‑149 (MQ=255)
                                                                                                 ggTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCt                                                                                                                              >  1:293032/1‑71 (MQ=255)
                                                                                                 ggTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCt                                                                                                                              <  2:293032/71‑1 (MQ=255)
                                                                                                           gCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTa                                                         <  8:266429/130‑1 (MQ=255)
                                                                                                           gCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTa                                                         >  7:266429/1‑130 (MQ=255)
                                                                                                            cACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATcc                                     >  5:183923/1‑149 (MQ=255)
                                                                                                                  ggtggtggtCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTc                                <  8:17848/148‑1 (MQ=255)
                                                                                                                          tCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTg                       >  1:133583/1‑149 (MQ=255)
                                                                                                                          tCAAGCGAGTTGCGTTATTCTATGAAGCGGTCGGCCATGCGTCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTg                       <  8:289262/149‑1 (MQ=255)
                                                                                                                            aaGCGCTTTGCGTTCTTCTCTGTAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTgc                      <  5:305480/148‑1 (MQ=255)
                                                                                                                            aaGCGCTTTGCGTTCTTCTCTGTAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTgc                      <  5:310052/148‑1 (MQ=255)
                                                                                                                            aaGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTg                                   <  5:75212/135‑1 (MQ=255)
                                                                                                                            aaGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTg                                   >  6:75212/1‑135 (MQ=255)
                                                                                                                                gcTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAAc                 >  5:94002/1‑149 (MQ=255)
                                                                                                                                     gCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTAc            >  5:298856/1‑149 (MQ=255)
                                                                                                                                      cGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTAcc           <  6:183923/149‑1 (MQ=255)
                                                                                                                                           ttctCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCt      >  4:158252/1‑149 (MQ=255)
                                                                                                                                             ctctGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTActgcctgc                                                 >  4:144815/1‑104 (MQ=255)
                                                                                                                                             ctctGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTActgcctgc                                                 <  3:144815/104‑1 (MQ=255)
                                                                                                                                              tctGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTc   >  1:2497/1‑149 (MQ=255)
                                                                                                                                                tGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTc   <  8:758/147‑1 (MQ=255)
                                                                                                                                                  aaGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCg  <  6:307026/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCAGGACTGGGCCACTGCCGGTTATCTGCCCGAGGCGCTGGCCTACGGTTTCGTGGTCAATGCCTTGCTGGCCGGCCTGATGATCGGCCCGGTGCTGGGTGGCCTGGGCACCTTGGTGGTGGTCAAGCGCTTTGCGTTCTTCTCTGAAGCGGTCGGCCATGCGGCGCTGACCGGCGTGGCCATCGGTATCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCG  >  NC_002947/4331330‑4331621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑