Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,340,076 G→C 26.3% intergenic (+61/‑270) ribAB‑II → / → PP_3814 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,340,0760GC26.3% 57.7 / 17.7 34intergenic (+61/‑270)ribAB‑II/PP_38143,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (12/13);  new base C (6/3);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

GGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACC  >  NC_002947/4339929‑4340214
                                                                                                                                                    |                                                                                                                                            
gggCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAgg                                                                                                                                             >  2:95875/1‑149 (MQ=255)
  gccgccGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTTACCGCGCATGGGAGGGt                                                                                                                                           >  3:272264/1‑149 (MQ=255)
     gccgcTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGa                                                                                                                                         >  5:314778/1‑148 (MQ=255)
        gcTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGacac                                                                                                                                      >  6:229896/1‑148 (MQ=255)
         cTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGAcacca                                                                                                                                    <  6:314778/149‑1 (MQ=255)
              aTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGAc                                                                                                                               <  8:115731/149‑1 (MQ=255)
              aTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGAc                                                                                                                               >  2:189022/1‑149 (MQ=255)
                       gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgcg                                                                                                                    <  1:189022/149‑1 (MQ=255)
                       gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcgcg                                                                                                                    <  8:186571/149‑1 (MQ=255)
                        cTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg                                                                                                                   <  3:313967/149‑1 (MQ=25)
                         tGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACACGCGAATGAGAGGGTGACACCACCGACCCATTACCggg                                                                                                                  >  7:214867/1‑149 (MQ=255)
                           gCGGGCTAAGACCTGGCGGGGGTGCAGATCATCCCGTTCGACCCGGGGATGGCTGGCTGCCAACACACAACCAAATGATTTAGCGGGGCCCTGTAGGACCGGGTTTACCCGCGAATTGCGAGGGTGACACCACCGACGCATTCGCGGGt                                                                                                                 <  1:42762/149‑1 (MQ=37)
                               gCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCAGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAAcc                                                                                                            <  1:35188/149‑1 (MQ=21)
                                   tGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCt                                                                                                        <  1:95875/149‑1 (MQ=255)
                                                     gagCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCACTGTAGGAGCGGGTTTAACACCGAATGCGACGGTGACAACACCCACGCAATACCCGGTTAACCCGCACCTACACGGCCCCAGGTg                                                                                      >  2:250028/1‑149 (MQ=255)
                                                                     ctggttatgtatgtCTTATAACACAAAACTATTTTATTTAGGGGGGCCTTTTAGTAGCGGGTTTACCCGTTAATGGTTGGGTTACACCACCTACTCTTTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGa                                                                      <  8:214867/143‑1 (MQ=255)
                                                                       ggggATGGATGGCTTACAACACACAACCAATTTATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGCGGGTGACACCACCGACGCTATTGGCGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAg                                                                     <  1:250028/149‑1 (MQ=37)
                                                                           atggatggCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTc                                                                                                       >  4:254389/1‑110 (MQ=255)
                                                                           atggatggCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTc                                                                                                       <  3:254389/110‑1 (MQ=255)
                                                                                               aaGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATTCGAGGGTGACACCACCGCCGGATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCCGTCGCCCCATACctct                                            >  5:62132/1‑149 (MQ=255)
                                                                                                            cgggggcCCCGGTCGGCGGGGGTTTCCCCGCTAAGGCTTGGGTCCCACCACCGCCCATTCCGGGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg                               <  4:197172/146‑1 (MQ=255)
                                                                                                             gCGGGGCCGTTTCGCCGCGGGTTCACCCGCTATTGGGCGGGTCACCCCACCGACGAATTCGCGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg                               <  7:21098/148‑1 (MQ=255)
                                                                                                                               gggTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGatat            >  1:63839/1‑149 (MQ=255)
                                                                                                                                 gTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATc           <  7:138220/148‑1 (MQ=255)
                                                                                                                                 gTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATc           >  8:138220/1‑148 (MQ=255)
                                                                                                                                       ccGCGAATGCTAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCATTGGTGCTTTTGACAg                                                                            >  8:184966/1‑77 (MQ=255)
                                                                                                                                       ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg                               >  1:243401/1‑122 (MQ=21)
                                                                                                                                       ccGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg                               <  2:243401/122‑1 (MQ=21)
                                                                                                                                       ccGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGa    >  2:153341/1‑149 (MQ=255)
                                                                                                                                       ccGAGAATGCGAGGGTGACACCACCGACGATTTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAg                                                                            <  7:184966/77‑1 (MQ=37)
                                                                                                                                        cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc   >  5:292495/1‑149 (MQ=25)
                                                                                                                                        cgcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc   >  8:192112/1‑149 (MQ=25)
                                                                                                                                         gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc  >  5:305866/1‑149 (MQ=25)
                                                                                                                                         gcgAATGCGTCGGTGGTGTCACCCTCGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCGTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc  >  6:101320/1‑149 (MQ=25)
                                                                                                                                         gcgAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc  >  4:195662/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACC  >  NC_002947/4339929‑4340214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: