| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,340,081 | A→G | 26.3% | intergenic (+66/‑265) | ribAB‑II → / → PP_3814 | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,340,081 | 0 | A | G | 26.3% | 41.4 / 14.4 | 31 | intergenic (+66/‑265) | ribAB‑II/PP_3814 | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (10/12); new base G (5/3); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
GCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACC > NC_002947/4339934‑4340214 | gccgcTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGa > 5:314778/1‑148 (MQ=255) gcTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGacac > 6:229896/1‑148 (MQ=255) cTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGAcacca < 6:314778/149‑1 (MQ=255) aTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGAc < 8:115731/149‑1 (MQ=255) aTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGAc > 2:189022/1‑149 (MQ=255) gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑cgcg < 1:189022/149‑1 (MQ=255) gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑cgcg < 8:186571/149‑1 (MQ=255) cTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCgg < 3:313967/149‑1 (MQ=25) tGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACACGCGAA‑TGAGAGGGTGACACCACCGACCC‑ATT‑ACCggg > 7:214867/1‑149 (MQ=255) gCGGGCTAAGACCTGGCGGGGGTGCAGATCATCCCGTTCGACCCGGGGATGGCTGGCTGCCAACACACAACCAAATGATTTAGCGGGGCCCTGTAGGACCGGGTTTACCCGCGAATTGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGt < 1:42762/149‑1 (MQ=37) gCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCAGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAAcc < 1:35188/149‑1 (MQ=21) tGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCt < 1:95875/149‑1 (MQ=255) gagCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCACTGTAGGAGCGGGTTTAACACCGAA‑TGCGACGGTGACAACACCCACGC‑AAT‑ACCCGGTTAACCCGCACCTACACGGCCCCAGGTg > 2:250028/1‑149 (MQ=255) ctggttatgtatgtCTTATAACACAAAACTATTTTATTTAGGGGGGCCTTTTAGTAGCGGGTTTACCCGTTAA‑TGGTTGGGTTACACCACCTACTC‑TTT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGa < 8:214867/143‑1 (MQ=255) ggggATGGATGGCTTACAACACACAACCAATTTATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGCGGGTGACACCACCGACGCTATT‑GGCGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAg < 1:250028/149‑1 (MQ=37) atggatggCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTc < 3:254389/110‑1 (MQ=255) atggatggCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTc > 4:254389/1‑110 (MQ=255) aaGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TTCGAGGGTGACACCACCGCCGG‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCCGTCGCCCCATACctct > 5:62132/1‑149 (MQ=255) cgggggcCCCGGTCGGCGGGGGTTTCCCCGCTAA‑GGCTTGGGTCCCACCACCG‑CCC‑ATTCCGGGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg < 4:197172/146‑1 (MQ=255) gCGGGGCCGTTTCGCCGCGGGTTCACCCGCTAT‑TGGGCGGGTCACCCCACCGACGA‑ATT‑CGCGGTTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg < 7:21098/148‑1 (MQ=255) gggTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGatat > 1:63839/1‑149 (MQ=255) gTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATc > 8:138220/1‑148 (MQ=255) gTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATc < 7:138220/148‑1 (MQ=255) ccGCGAA‑TGCTAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCATTGGTGCTTTTGACAg > 8:184966/1‑77 (MQ=255) ccGCGAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg > 1:243401/1‑122 (MQ=21) ccGCGAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTg < 2:243401/122‑1 (MQ=21) ccGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGa > 2:153341/1‑149 (MQ=255) ccGAGAA‑TGCGAGGGTGACACCACCGACGA‑TTT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAg < 7:184966/77‑1 (MQ=37) cgcgAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc > 5:292495/1‑149 (MQ=25) cgcgAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAc > 8:192112/1‑149 (MQ=25) gcgAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc > 5:305866/1‑149 (MQ=25) gcgAA‑TGCGTCGGTGGTGTCACCCTCGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCGTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc > 6:101320/1‑149 (MQ=25) gcgAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGAcc > 4:195662/1‑149 (MQ=255) | GCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAA‑TGCGAGGGTGACACCACCGACGC‑ATT‑CGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCGGTCGCCCCATACCTCTTGCCAAAATCTTGGAATACCATAATATGATATCCCGTAGACC > NC_002947/4339934‑4340214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |