Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,362,611 T→A 7.8% intergenic (‑132/‑304) PP_5634 ← / → adhP hypothetical protein/alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,362,6110TA 7.8% 144.2 / 2.3 51intergenic (‑132/‑304)PP_5634/adhPhypothetical protein/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base A (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TCGTCTTCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCATC  >  NC_002947/4362463‑4362747
                                                                                                                                                    |                                                                                                                                        
tcgtcTTCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGACTTTTGCCCGCCt                                                                                                                                          >  3:35463/1‑149 (MQ=255)
   tcTTCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTc                                                                                                                                        >  2:2105/1‑148 (MQ=255)
     ttCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCATTCCAt                                                                                                                                     >  6:306881/1‑149 (MQ=255)
         tcgCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCg                                                                                                                                 >  1:9825/1‑149 (MQ=255)
           gcgcATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCCCAACCTGCAGGCTCTCCCTTCAAACCTCTTTAGCCCGCCTTCCATGGCggg                                                                                                                               >  5:221961/1‑149 (MQ=255)
                         cTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGAcc                                                                                                                  <  3:32111/148‑1 (MQ=255)
                              aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTgcgc                                                                                                            <  7:259617/149‑1 (MQ=255)
                              aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                              <  4:33678/131‑1 (MQ=255)
                              aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                              >  3:33678/1‑131 (MQ=255)
                              aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                              <  2:318815/131‑1 (MQ=255)
                              aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                              >  1:318815/1‑131 (MQ=255)
                               tAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCa                                                                                                           >  8:97694/1‑149 (MQ=255)
                                 aTGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACt                                                                                                         <  1:126755/149‑1 (MQ=255)
                                  tGGCACGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGAGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTTCAGGCTCTCCCTTCAAACGTCTATATCCAGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACtt                                                                                                        >  5:244175/1‑149 (MQ=255)
                                   ggCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTc                                                                                                       >  5:330156/1‑149 (MQ=255)
                                          cTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAg                                                                                                <  2:9825/149‑1 (MQ=255)
                                            gTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgat                                                                                              >  1:31863/1‑149 (MQ=255)
                                                  gCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGAtgatga                                                                                         >  2:82813/1‑148 (MQ=255)
                                                          gggTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAt                                                                                <  6:330156/149‑1 (MQ=255)
                                                          gggTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAt                                                                                <  1:2105/149‑1 (MQ=255)
                                                           ggTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAt                                                                                >  8:184380/1‑148 (MQ=255)
                                                                  ggTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAtgacctgac                                                                        <  2:31863/149‑1 (MQ=255)
                                                                         gCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGc                                                                                                  >  2:10348/1‑116 (MQ=255)
                                                                         gCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGc                                                                                                  <  1:10348/116‑1 (MQ=255)
                                                                            tGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGaaa                                                              <  5:295223/149‑1 (MQ=255)
                                                                                 gCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTc                                                          <  1:312492/148‑1 (MQ=255)
                                                                                 gCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTc                                                          <  7:184380/148‑1 (MQ=255)
                                                                                 gCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTc                                                          <  5:47863/148‑1 (MQ=255)
                                                                                  cTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCGATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGa                                                        >  1:101018/1‑149 (MQ=255)
                                                                                   ttAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgat                                                                                              >  1:150276/1‑110 (MQ=255)
                                                                                   ttAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgat                                                                                              <  2:150276/110‑1 (MQ=255)
                                                                                         cTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCg                                                  >  8:241150/1‑148 (MQ=255)
                                                                                         cTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCg                                                  >  2:121749/1‑148 (MQ=255)
                                                                                             gTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATgg                                             <  1:121749/149‑1 (MQ=255)
                                                                                               tAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATggg                                            >  5:78030/1‑148 (MQ=255)
                                                                                                   cggaccggCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCacac                                       <  7:97694/149‑1 (MQ=255)
                                                                                                          gcgcAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCt                                <  5:120394/149‑1 (MQ=255)
                                                                                                                   gCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAg                                                                                                <  1:46485/76‑1 (MQ=255)
                                                                                                                   gCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAg                                                                                                >  2:46485/1‑76 (MQ=255)
                                                                                                                    cAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTAt                       <  7:71648/148‑1 (MQ=255)
                                                                                                                       gCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTc                   >  2:29456/1‑149 (MQ=255)
                                                                                                                             ccTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAg             >  5:124375/1‑149 (MQ=255)
                                                                                                                              cTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATc                                                    <  4:76283/109‑1 (MQ=255)
                                                                                                                              cTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATc                                                    >  3:76283/1‑109 (MQ=255)
                                                                                                                              cTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGACACACTGGCCCTGCTCCGTATGTTCTTGCAGc            >  1:65428/1‑149 (MQ=255)
                                                                                                                                tCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCgg                                                 >  1:303181/1‑110 (MQ=255)
                                                                                                                                tCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCgg                                                 <  2:303181/110‑1 (MQ=255)
                                                                                                                                   aaCGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAtgacctga                                                                         >  7:145217/1‑83 (MQ=255)
                                                                                                                                   aaCGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAtgacctga                                                                         <  8:145217/83‑1 (MQ=255)
                                                                                                                                      gTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCa    >  2:203363/1‑149 (MQ=255)
                                                                                                                                       tCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCAt   <  1:203363/149‑1 (MQ=255)
                                                                                                                                        cTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTGCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCATc  <  4:35463/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
TCGTCTTCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCATC  >  NC_002947/4362463‑4362747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑