Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,362,616 T→A 7.7% intergenic (‑137/‑299) PP_5634 ← / → adhP hypothetical protein/alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,362,6160TA 7.7% 148.4 / 2.8 52intergenic (‑137/‑299)PP_5634/adhPhypothetical protein/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base A (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TTCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCATCCTTGCCGCGGGCTTT  >  NC_002947/4362468‑4362762
                                                                                                                                                    |                                                                                                                                                  
ttCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCATTCCAt                                                                                                                                                    >  6:306881/1‑149 (MQ=255)
    tcgCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCg                                                                                                                                                >  1:9825/1‑149 (MQ=255)
      gcgcATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCCCAACCTGCAGGCTCTCCCTTCAAACCTCTTTAGCCCGCCTTCCATGGCggg                                                                                                                                              >  5:221961/1‑149 (MQ=255)
                    cTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGAcc                                                                                                                                 <  3:32111/148‑1 (MQ=255)
                         aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTgcgc                                                                                                                           <  7:259617/149‑1 (MQ=255)
                         aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                                             <  4:33678/131‑1 (MQ=255)
                         aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                                             >  3:33678/1‑131 (MQ=255)
                         aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                                             <  2:318815/131‑1 (MQ=255)
                         aTAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCATGGAAGGCGGGc                                                                                                                                             >  1:318815/1‑131 (MQ=255)
                          tAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCa                                                                                                                          >  8:97694/1‑149 (MQ=255)
                            aTGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACt                                                                                                                        <  1:126755/149‑1 (MQ=255)
                             tGGCACGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGAGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTTCAGGCTCTCCCTTCAAACGTCTATATCCAGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACtt                                                                                                                       >  5:244175/1‑149 (MQ=255)
                              ggCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTc                                                                                                                      >  5:330156/1‑149 (MQ=255)
                                     cTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAg                                                                                                               <  2:9825/149‑1 (MQ=255)
                                       gTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgat                                                                                                             >  1:31863/1‑149 (MQ=255)
                                             gCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGAtgatga                                                                                                        >  2:82813/1‑148 (MQ=255)
                                                     gggTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAt                                                                                               <  6:330156/149‑1 (MQ=255)
                                                     gggTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAt                                                                                               <  1:2105/149‑1 (MQ=255)
                                                      ggTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAt                                                                                               >  8:184380/1‑148 (MQ=255)
                                                             ggTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAtgacctgac                                                                                       <  2:31863/149‑1 (MQ=255)
                                                                    gCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGc                                                                                                                 <  1:10348/116‑1 (MQ=255)
                                                                    gCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGc                                                                                                                 >  2:10348/1‑116 (MQ=255)
                                                                       tGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGaaa                                                                             <  5:295223/149‑1 (MQ=255)
                                                                            gCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTc                                                                         <  1:312492/148‑1 (MQ=255)
                                                                            gCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTc                                                                         <  7:184380/148‑1 (MQ=255)
                                                                            gCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTc                                                                         <  5:47863/148‑1 (MQ=255)
                                                                             cTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCGATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGa                                                                       >  1:101018/1‑149 (MQ=255)
                                                                              ttAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgat                                                                                                             <  2:150276/110‑1 (MQ=255)
                                                                              ttAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgat                                                                                                             >  1:150276/1‑110 (MQ=255)
                                                                                    cTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCg                                                                 >  8:241150/1‑148 (MQ=255)
                                                                                    cTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCg                                                                 >  2:121749/1‑148 (MQ=255)
                                                                                        gTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATgg                                                            <  1:121749/149‑1 (MQ=255)
                                                                                          tAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATggg                                                           >  5:78030/1‑148 (MQ=255)
                                                                                              cggaccggCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCacac                                                      <  7:97694/149‑1 (MQ=255)
                                                                                                     gcgcAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCt                                               <  5:120394/149‑1 (MQ=255)
                                                                                                              gCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAg                                                                                                               <  1:46485/76‑1 (MQ=255)
                                                                                                              gCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAg                                                                                                               >  2:46485/1‑76 (MQ=255)
                                                                                                               cAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTAt                                      <  7:71648/148‑1 (MQ=255)
                                                                                                                  gCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTc                                  >  2:29456/1‑149 (MQ=255)
                                                                                                                        ccTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAg                            >  5:124375/1‑149 (MQ=255)
                                                                                                                         cTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATc                                                                   <  4:76283/109‑1 (MQ=255)
                                                                                                                         cTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATc                                                                   >  3:76283/1‑109 (MQ=255)
                                                                                                                         cTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGACACACTGGCCCTGCTCCGTATGTTCTTGCAGc                           >  1:65428/1‑149 (MQ=255)
                                                                                                                           tCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCgg                                                                >  1:303181/1‑110 (MQ=255)
                                                                                                                           tCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCgg                                                                <  2:303181/110‑1 (MQ=255)
                                                                                                                              aaCGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAtgacctga                                                                                        >  7:145217/1‑83 (MQ=255)
                                                                                                                              aaCGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCAtgacctga                                                                                        <  8:145217/83‑1 (MQ=255)
                                                                                                                                 gTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCa                   >  2:203363/1‑149 (MQ=255)
                                                                                                                                  tCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCAt                  <  1:203363/149‑1 (MQ=255)
                                                                                                                                   cTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTGCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCATc                 <  4:35463/149‑1 (MQ=255)
                                                                                                                                                  cATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgatg                                                                                                            <  3:272806/43‑1 (MQ=255)
                                                                                                                                                  cATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAgatg                                                                                                            >  4:272806/1‑43 (MQ=255)
                                                                                                                                                  cATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCGGCAAGGATGCGGCGGGctgc  <  6:136252/149‑3 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTCGTCGCGCATGCTGGCTCCTGGGATAATGGCAGGCCTGTATAGGCTGATCAGGGTGCCGGGTCAATGCGTGGTCGCTTAATCCTGGGTTAACCGGACCGGCGCAACCTGCAGGCTCTCCCTTCAAACGTCTTTTGCCCGCCTTCCATGGCGGGCTTTTTTATGACCCTGCGCACTTCGTTGCAGATGATGACAGCCTCATGACCTGACCTTCACGAAATGTCGACATCCGGATGGGCACACTGGCCCTGCTCCGTATGTTCTTGCAGCCCGCCGCATCCTTGCCGCGGGCTTT  >  NC_002947/4362468‑4362762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑