Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,509,116 C→G 8.9% E316Q (GAG→CAG)  serS ← serine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,509,1160CG 8.9% 131.9 / 4.6 45E316Q (GAG→CAG) serSserine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base G (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

CGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCGG  >  NC_002947/4508968‑4509262
                                                                                                                                                    |                                                                                                                                                  
cggcgCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTc                                                                                                                                                    <  5:256196/149‑1 (MQ=255)
  gcgcgcCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCca                                                                                                                                                  <  7:143865/149‑1 (MQ=255)
  gcgcgcCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCca                                                                                                                                                  <  2:285697/149‑1 (MQ=255)
        cTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCt                                                                                                                                            >  6:262685/1‑149 (MQ=255)
           gAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTgc                                                                                                                                          <  4:313732/148‑1 (MQ=255)
            aaGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTGCTGGAGCTgcca                                                                                                                                        <  1:167356/149‑3 (MQ=255)
              gTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGTGCGAGCACGCGGTATGGCAGCTCCAGCAGCTgcagca                                                                                                                                      <  8:55707/149‑1 (MQ=255)
              gTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTgcagca                                                                                                                                      >  6:181248/1‑149 (MQ=255)
                 accacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCAATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTACAGCAcgc                                                                                                                                   >  3:291497/1‑149 (MQ=255)
                  ccacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCAcgcg                                                                                                                                  >  4:152145/1‑149 (MQ=255)
                      aGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCg                                                                                                                              <  3:152145/149‑1 (MQ=255)
                           gAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCAtt                                                                                                                         <  7:270750/149‑1 (MQ=255)
                             gCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTACAGCACGCGCTCGGCATTgg                                                                                                                       <  4:291497/149‑1 (MQ=255)
                                     tGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGCTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCCGCAGCACGCGCTCGGCATTCGCAGTCAgg                                                                                                               >  1:207069/1‑149 (MQ=255)
                                            gcgGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTc                                                                                                          >  5:278193/1‑147 (MQ=255)
                                                     gTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTcc                                                                                                <  5:181248/148‑1 (MQ=255)
                                                        cTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATc                                                                                             <  8:4185/148‑1 (MQ=255)
                                                        cTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATc                                                                                             >  6:185382/1‑148 (MQ=255)
                                                          ggCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCa                                                                                                                     <  8:159138/122‑1 (MQ=255)
                                                          ggCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCa                                                                                                                     >  7:159138/1‑122 (MQ=255)
                                                             tCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTg                                                                                       >  5:146991/1‑149 (MQ=255)
                                                               ggCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTgg                                                                                      <  5:185382/148‑1 (MQ=255)
                                                                      acacTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGctct                                                                              <  5:262685/149‑1 (MQ=255)
                                                                      acacTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGctct                                                                              <  6:278193/149‑1 (MQ=255)
                                                                                    tAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc                                                                                                                               >  7:242746/1‑86 (MQ=255)
                                                                                    tAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc                                                                                                                               <  8:242746/86‑1 (MQ=255)
                                                                                            cACCGCACCTAAGCCAATGTCGCCAGTGCACAGGGCCAGCACGCTGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGTACTTCCTGGGCTTCCATCGACTTGGACGGCTCTACCACCTTCACCATCTCGACt                                                         <  6:146991/148‑1 (MQ=255)
                                                                                              ccGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTg                                                      >  6:268750/1‑149 (MQ=255)
                                                                                               cGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGAACGAGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTGCCATCGACTTGGACGGCTCTACCACCTGCTCCATCTCGACTgtgt                                                     >  5:243771/1‑149 (MQ=255)
                                                                                                          ccATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGc                                          >  8:260821/1‑149 (MQ=255)
                                                                                                           cATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCt                                         >  7:1527/1‑149 (MQ=255)
                                                                                                             tGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCAATTCGACAAAGTCGAGATGGTGCAgg                                       <  4:190091/149‑1 (MQ=255)
                                                                                                                cGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACgg                                                                                  >  1:254766/1‑103 (MQ=255)
                                                                                                                cGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACgg                                                                                  <  2:254766/103‑1 (MQ=255)
                                                                                                                 gCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGa                                   >  6:291338/1‑149 (MQ=255)
                                                                                                                      tGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATg                              <  8:1527/149‑1 (MQ=255)
                                                                                                                          caGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCcgc                          >  2:268843/1‑149 (MQ=255)
                                                                                                                            gggCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCgg                        >  8:207950/1‑149 (MQ=255)
                                                                                                                                     cgcgGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGGCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCGCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACgg               <  5:255879/149‑1 (MQ=255)
                                                                                                                                         gtgctgCAGCTGCTGGAGCTGCCGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGc                                      <  7:81939/117‑1 (MQ=255)
                                                                                                                                         gtgctgCAGCTGCTGGAGCTGCCGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGc                                      >  8:81939/6‑122 (MQ=255)
                                                                                                                                           gctgCAGCTGCTGGAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGaa         >  8:287218/4‑149 (MQ=255)
                                                                                                                                              gCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAgcg      >  5:25699/1‑149 (MQ=255)
                                                                                                                                                aGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCAc                 >  6:305392/1‑136 (MQ=255)
                                                                                                                                                aGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCAc                 <  5:305392/136‑1 (MQ=255)
                                                                                                                                                  cTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCgg  <  7:207950/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCGG  >  NC_002947/4508968‑4509262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑