Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,509,118	A→T	9.3%	L315Q (CTG→CAG)	serS ←	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,509,118	0	A	T	9.3%	116.7 / 2.8	43	L315Q (CTG→CAG)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (20/19);  new base T (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

GCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCGG  >  NC_002947/4508970‑4509262                                                                                                                                                     |                                                                                                                                                 gcgcgcCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCca                                                                                                                                                  <  7:143865/149‑1 (MQ=255) gcgcgcCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCca                                                                                                                                                  <  2:285697/149‑1 (MQ=255)       cTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCt                                                                                                                                            >  6:262685/1‑149 (MQ=255)          gAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTgc                                                                                                                                          <  4:313732/148‑1 (MQ=255)           aaGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTGCTGGAGCTgcca                                                                                                                                        <  1:167356/149‑3 (MQ=255)             gTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGTGCGAGCACGCGGTATGGCAGCTCCAGCAGCTgcagca                                                                                                                                      <  8:55707/149‑1 (MQ=255)             gTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTgcagca                                                                                                                                      >  6:181248/1‑149 (MQ=255)                accacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCAATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTACAGCAcgc                                                                                                                                   >  3:291497/1‑149 (MQ=255)                 ccacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCAcgcg                                                                                                                                  >  4:152145/1‑149 (MQ=255)                     aGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCg                                                                                                                              <  3:152145/149‑1 (MQ=255)                          gAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCAtt                                                                                                                         <  7:270750/149‑1 (MQ=255)                            gCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTACAGCACGCGCTCGGCATTgg                                                                                                                       <  4:291497/149‑1 (MQ=255)                                    tGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGCTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCCGCAGCACGCGCTCGGCATTCGCAGTCAgg                                                                                                               >  1:207069/1‑149 (MQ=255)                                           gcgGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTc                                                                                                          >  5:278193/1‑147 (MQ=255)                                                    gTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTcc                                                                                                <  5:181248/148‑1 (MQ=255)                                                       cTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATc                                                                                             >  6:185382/1‑148 (MQ=255)                                                       cTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATc                                                                                             <  8:4185/148‑1 (MQ=255)                                                         ggCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCa                                                                                                                     <  8:159138/122‑1 (MQ=255)                                                         ggCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCa                                                                                                                     >  7:159138/1‑122 (MQ=255)                                                            tCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTg                                                                                       >  5:146991/1‑149 (MQ=255)                                                              ggCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTgg                                                                                      <  5:185382/148‑1 (MQ=255)                                                                     acacTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGctct                                                                              <  6:278193/149‑1 (MQ=255)                                                                     acacTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGctct                                                                              <  5:262685/149‑1 (MQ=255)                                                                                   tAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc                                                                                                                               >  7:242746/1‑86 (MQ=255)                                                                                   tAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc                                                                                                                               <  8:242746/86‑1 (MQ=255)                                                                                           cACCGCACCTAAGCCAATGTCGCCAGTGCACAGGGCCAGCACGCTGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGTACTTCCTGGGCTTCCATCGACTTGGACGGCTCTACCACCTTCACCATCTCGACt                                                         <  6:146991/148‑1 (MQ=255)                                                                                             ccGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTg                                                      >  6:268750/1‑149 (MQ=255)                                                                                              cGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGAACGAGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTGCCATCGACTTGGACGGCTCTACCACCTGCTCCATCTCGACTgtgt                                                     >  5:243771/1‑149 (MQ=255)                                                                                                         ccATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGc                                          >  8:260821/1‑149 (MQ=255)                                                                                                          cATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCt                                         >  7:1527/1‑149 (MQ=255)                                                                                                            tGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCAATTCGACAAAGTCGAGATGGTGCAgg                                       <  4:190091/149‑1 (MQ=255)                                                                                                               cGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACgg                                                                                  >  1:254766/1‑103 (MQ=255)                                                                                                               cGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACgg                                                                                  <  2:254766/103‑1 (MQ=255)                                                                                                                gCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGa                                   >  6:291338/1‑149 (MQ=255)                                                                                                                     tGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATg                              <  8:1527/149‑1 (MQ=255)                                                                                                                         caGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCcgc                          >  2:268843/1‑149 (MQ=255)                                                                                                                           gggCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCgg                        >  8:207950/1‑149 (MQ=255)                                                                                                                                    cgcgGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGGCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCGCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACgg               <  5:255879/149‑1 (MQ=255)                                                                                                                                        gtgctgCAGCTGCTGGAGCTGCCGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGc                                      >  8:81939/6‑122 (MQ=255)                                                                                                                                        gtgctgCAGCTGCTGGAGCTGCCGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGc                                      <  7:81939/117‑1 (MQ=255)                                                                                                                                          gctgCAGCTGCTGGAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGaa         >  8:287218/4‑149 (MQ=255)                                                                                                                                             gCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAgcg      >  5:25699/1‑149 (MQ=255)                                                                                                                                               aGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCAc                 >  6:305392/1‑136 (MQ=255)                                                                                                                                               aGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCAc                 <  5:305392/136‑1 (MQ=255)                                                                                                                                                 cTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCgg  <  7:207950/149‑1 (MQ=255)                                                                                                                                                    cagcagCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGt                                            <  4:9952/104‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCGG  >  NC_002947/4508970‑4509262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑