Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,509,120	C→G	9.3%	L314L (CTG→CTC)	serS ←	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,509,120	0	C	G	9.3%	126.7 / 4.7	43	L314L (CTG→CTC)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base G (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

CTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCGG  >  NC_002947/4508976‑4509262                                                                                                                                                 |                                                                                                                                               cTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCt                                                                                                                                            >  6:262685/1‑149 (MQ=255)    gAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTgc                                                                                                                                          <  4:313732/148‑1 (MQ=255)     aaGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTGCTGGAGCTgcca                                                                                                                                        <  1:167356/149‑3 (MQ=255)       gTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGTGCGAGCACGCGGTATGGCAGCTCCAGCAGCTgcagca                                                                                                                                      <  8:55707/149‑1 (MQ=255)       gTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTgcagca                                                                                                                                      >  6:181248/1‑149 (MQ=255)          accacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCAATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTACAGCAcgc                                                                                                                                   >  3:291497/1‑149 (MQ=255)           ccacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCAcgcg                                                                                                                                  >  4:152145/1‑149 (MQ=255)               aGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCg                                                                                                                              <  3:152145/149‑1 (MQ=255)                    gAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCAtt                                                                                                                         <  7:270750/149‑1 (MQ=255)                      gCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTACAGCACGCGCTCGGCATTgg                                                                                                                       <  4:291497/149‑1 (MQ=255)                              tGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGCTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCCGCAGCACGCGCTCGGCATTCGCAGTCAgg                                                                                                               >  1:207069/1‑149 (MQ=255)                                     gcgGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTc                                                                                                          >  5:278193/1‑147 (MQ=255)                                              gTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTcc                                                                                                <  5:181248/148‑1 (MQ=255)                                                 cTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATc                                                                                             <  8:4185/148‑1 (MQ=255)                                                 cTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATc                                                                                             >  6:185382/1‑148 (MQ=255)                                                   ggCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCa                                                                                                                     <  8:159138/122‑1 (MQ=255)                                                   ggCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCa                                                                                                                     >  7:159138/1‑122 (MQ=255)                                                      tCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTg                                                                                       >  5:146991/1‑149 (MQ=255)                                                        ggCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTgg                                                                                      <  5:185382/148‑1 (MQ=255)                                                               acacTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGctct                                                                              <  6:278193/149‑1 (MQ=255)                                                               acacTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGctct                                                                              <  5:262685/149‑1 (MQ=255)                                                                             tAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc                                                                                                                               <  8:242746/86‑1 (MQ=255)                                                                             tAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc                                                                                                                               >  7:242746/1‑86 (MQ=255)                                                                                     cACCGCACCTAAGCCAATGTCGCCAGTGCACAGGGCCAGCACGCTGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGTACTTCCTGGGCTTCCATCGACTTGGACGGCTCTACCACCTTCACCATCTCGACt                                                         <  6:146991/148‑1 (MQ=255)                                                                                       ccGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTg                                                      >  6:268750/1‑149 (MQ=255)                                                                                        cGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGAACGAGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTGCCATCGACTTGGACGGCTCTACCACCTGCTCCATCTCGACTgtgt                                                     >  5:243771/1‑149 (MQ=255)                                                                                                   ccATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGc                                          >  8:260821/1‑149 (MQ=255)                                                                                                    cATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCt                                         >  7:1527/1‑149 (MQ=255)                                                                                                      tGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCAATTCGACAAAGTCGAGATGGTGCAgg                                       <  4:190091/149‑1 (MQ=255)                                                                                                         cGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACgg                                                                                  >  1:254766/1‑103 (MQ=255)                                                                                                         cGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACgg                                                                                  <  2:254766/103‑1 (MQ=255)                                                                                                          gCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGa                                   >  6:291338/1‑149 (MQ=255)                                                                                                               tGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATg                              <  8:1527/149‑1 (MQ=255)                                                                                                                   caGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCcgc                          >  2:268843/1‑149 (MQ=255)                                                                                                                     gggCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCgg                        >  8:207950/1‑149 (MQ=255)                                                                                                                              cgcgGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGGCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCGCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACgg               <  5:255879/149‑1 (MQ=255)                                                                                                                                  gtgctgCAGCTGCTGGAGCTGCCGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGc                                      >  8:81939/6‑122 (MQ=255)                                                                                                                                  gtgctgCAGCTGCTGGAGCTGCCGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGc                                      <  7:81939/117‑1 (MQ=255)                                                                                                                                    gctgCAGCTGCTGGAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGaa         >  8:287218/4‑149 (MQ=255)                                                                                                                                       gCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAgcg      >  5:25699/1‑149 (MQ=255)                                                                                                                                         aGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCAc                 <  5:305392/136‑1 (MQ=255)                                                                                                                                         aGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCAc                 >  6:305392/1‑136 (MQ=255)                                                                                                                                           cTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCgg  <  7:207950/149‑1 (MQ=255)                                                                                                                                              cagcagCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGt                                            <  4:9952/104‑1 (MQ=255)                                                                                                                                                gcagcTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTc                                                    <  5:217649/94‑1 (MQ=255)                                                                                                                                                gcagcTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTc                                                    >  6:217649/1‑94 (MQ=255)                                                                                                                                                 |                                                                                                                                               CTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGTCAGGCCTTCCAGGGCTTCCATCGACTTGGACGGCTCTACCACCTGCACCATCTCGACTTTGTCGAATTGGTGCTGGCGGATCATGCCGCGGGTGTCACGGCCAGAAGCGCCGG  >  NC_002947/4508976‑4509262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑