Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,512,465 T→G 10.6% N8T (AAC→ACC)  lolA ← outer membrane lipoprotein carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,512,4650TG10.6% 146.2 / 10.6 57N8T (AAC→ACC) lolAouter membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base G (2/4);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

ACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCA  >  NC_002947/4512319‑4512608
                                                                                                                                                  |                                                                                                                                               
aCTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTGAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTg                                                                                                                                               <  6:173568/149‑1 (MQ=255)
aCTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTg                                                                                                                                               <  4:238088/149‑1 (MQ=255)
       cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcag                                                                                                                                        >  7:79183/1‑149 (MQ=255)
       cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                        <  5:318191/149‑1 (MQ=255)
       cAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcag                                                                                                                                        <  2:158746/149‑1 (MQ=255)
        aGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcagg                                                                                                                                       <  7:236030/149‑1 (MQ=255)
         gaaaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTAGAAAAGGCAAGGCGCCACCGTTGGAGCcagg                                                                                                                                       >  2:97474/3‑148 (MQ=255)
           aaCTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcag                                                                                                                                    <  7:253965/149‑1 (MQ=255)
                ggTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggcctttt                                                                                                                                 <  7:137927/147‑6 (MQ=255)
                  tCAGGCGTTGTATGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtc                                                                                                                             <  8:175303/149‑1 (MQ=255)
                  tCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcagg                                                                                                                                   <  5:295560/143‑1 (MQ=255)
                  tCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCcaggcagg                                                                                                                                   >  6:295560/1‑143 (MQ=255)
                    aGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                           <  7:167248/149‑1 (MQ=255)
                     ggCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTtcat                                                                                                                           >  3:246019/1‑148 (MQ=255)
                         ttGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTc                                                                                                                      >  6:12701/1‑149 (MQ=255)
                          tGTAAGTCTTTCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCTGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGATATGGCGTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTc                                                                                                                      <  4:210892/148‑1 (MQ=255)
                               gTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTcgcac                                                                                                                  >  1:114859/1‑145 (MQ=255)
                                 cTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGgc                                                                                                              >  3:102242/1‑149 (MQ=255)
                                  tagcccACCGGCATACGCAGTAACCGAGCCCAGCGGCAGGCCAGAAACCAACACCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCggg                                                                                                               <  4:246019/142‑1 (MQ=255)
                                    gCTCACCGGCATAAGCTGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGTCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGgc                                                                                                              <  1:97474/146‑1 (MQ=255)
                                      tCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTCAAAAGGCCTTCCGCCACCGATGGCGCCAGGCAGGCTGTTCATCAGTCGCGCGGGCCGccc                                                                                                         >  2:20485/1‑149 (MQ=255)
                                        aCCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggc                                                                                                                                      >  8:26819/1‑118 (MQ=255)
                                        aCCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCAACGGTGGCGCcaggc                                                                                                                                      <  7:26819/118‑1 (MQ=255)
                                          cGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgggg                                                                                                     <  2:114859/149‑1 (MQ=255)
                                          cGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCggg                                                                                                      >  4:119730/1‑148 (MQ=255)
                                          cGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCggg                                                                                                      <  3:119730/148‑1 (MQ=255)
                                                 aGCAGTAACCGAGCCCAGGGTCTGGGCAGATACCAACAGCATGCGTATCGCGCGCATGGGAAGCCTCATTGAGCGTTGTATAGGCCTGGAGCCACCGTTGGCGCCAGGGAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTAtga                                                                                               <  4:305711/148‑3 (MQ=255)
                                                       aaCCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCcg                                                                                        >  3:6216/1‑149 (MQ=255)
                                                         ccGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACtt                                                                                            <  7:213435/143‑1 (MQ=255)
                                                         ccGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACtt                                                                                            >  8:213435/1‑143 (MQ=255)
                                                          cGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCcgc                                                                                       >  7:119376/1‑147 (MQ=255)
                                                          cGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGa                                                                                     <  5:12701/149‑1 (MQ=255)
                                                              cccAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCg                                                                                 <  7:120151/149‑1 (MQ=255)
                                                                cAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCCCGAGCCGtt                                                                               >  2:314262/1‑149 (MQ=255)
                                                                   ggTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCt                                                                            <  8:119376/149‑1 (MQ=255)
                                                                         ggCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCAt                                                                      >  8:204920/1‑149 (MQ=255)
                                                                               aaCCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGt                                                                <  4:6216/149‑1 (MQ=255)
                                                                                     cAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAAc                                                          <  1:314262/149‑1 (MQ=255)
                                                                                       gCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGc                                                        <  4:102242/149‑1 (MQ=255)
                                                                                        cATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGcc                                                       >  2:293105/1‑149 (MQ=255)
                                                                                               aTCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgg                                                                                                       >  2:43265/1‑94 (MQ=255)
                                                                                               aTCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCgg                                                                                                       <  1:43265/94‑1 (MQ=255)
                                                                                                tCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCAtct                                               >  4:286465/1‑149 (MQ=255)
                                                                                                                  tCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgc                             >  8:236193/1‑149 (MQ=255)
                                                                                                                       gAGCGTTGAAAAGGCGTGGCGCCACCGTTGGCGCCAGGCAGGGTGGTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATgcgcgc                         <  1:20485/148‑1 (MQ=255)
                                                                                                                              gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                          <  7:136724/124‑1 (MQ=255)
                                                                                                                              gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATc                                          >  8:136724/1‑124 (MQ=255)
                                                                                                                              gAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACgg                  <  3:286465/148‑1 (MQ=255)
                                                                                                                               aaaaGGCCTGGCGCCACCGTTGGCGCTAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                >  8:211040/1‑149 (MQ=255)
                                                                                                                               aaaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                >  7:47092/1‑149 (MQ=255)
                                                                                                                                aaaGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGtt                <  8:47092/148‑1 (MQ=255)
                                                                                                                                     ccTGGCGCCACCGTTGGCGCTAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGcc          <  7:211040/149‑1 (MQ=255)
                                                                                                                                     ccTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTAGCGAGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTAGATCATGCGCGCGGCACGGTTTTAGcc          >  5:183608/1‑149 (MQ=255)
                                                                                                                                     ccTGGCGCCAACGGTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGCCTCGATCATGCGCGCGGCACGGTTGTAGcc          >  1:29020/1‑149 (MQ=255)
                                                                                                                                         gcgcCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATc      >  5:323666/1‑149 (MQ=255)
                                                                                                                                             cACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCa  >  8:217846/1‑149 (MQ=255)
                                                                                                                                             cACCGTTGCCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTAGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCa  <  3:35249/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACTTTTCCAGCAACTGGGTCAGGCGTTGTACGTCTTGCTCACCGGCATAAGCAGTAACCGAGCCCAGGGTCAGGGCAGAAACCAACAGCATGCGAATCGCGCGCATGGGAATCCTCATTGAGCGTTGAAAAGGCCTGGCGCCACCGTTGGCGCCAGGCAGGGTGTTCATCAGTCGCGCGGGCCGCCCGGGGCTATCACTTCCCGCGAGCCGTTGCTGTTCATCGGGGTGACAACGCCGGCCATCTCCATCGACTCGATCATGCGCGCGGCACGGTTGTAGCCGATCTTCA  >  NC_002947/4512319‑4512608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑