Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 354,867 G→C 9.7% T102R (ACG→AGG)  cbcW ← choline/betaine/carnitine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947354,8670GC 9.7% 146.2 / 4.8 51T102R (ACG→AGG) cbcWcholine/betaine/carnitine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base C (2/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CAGGCCGAACAGGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGA  >  NC_002947/354719‑355008
                                                                                                                                                     |                                                                                                                                             
cAGGCCGAACAGGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCg                                                                                                                                               >  5:67699/1‑149 (MQ=255)
   gCCGAACAGGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTgg                                                                                                                                            <  6:9059/149‑1 (MQ=255)
             ggcatggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGAc                                                                                                                                  <  3:210155/144‑1 (MQ=255)
                       ggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAg                                                                                                                         <  1:304241/148‑1 (MQ=255)
                            aGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGtctc                                                                                                                   <  5:190036/149‑1 (MQ=255)
                              gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGtggt                                                                                                                   <  3:284064/147‑4 (MQ=255)
                              gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGtggt                                                                                                                   >  4:284064/1‑144 (MQ=255)
                                 gACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                               <  5:287395/148‑1 (MQ=255)
                                      aGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                          >  1:62776/1‑149 (MQ=255)
                                         tGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      <  5:99424/149‑1 (MQ=255)
                                         tGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      <  8:19854/149‑1 (MQ=255)
                                         tGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      <  2:62776/149‑1 (MQ=255)
                                           ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      >  3:287317/1‑147 (MQ=255)
                                           ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      <  4:287317/147‑1 (MQ=255)
                                             cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAg                                                                                                   >  3:112415/1‑148 (MQ=255)
                                                      cATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGt                                                                                         >  4:250403/1‑149 (MQ=255)
                                                               gAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGAACACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATca                                                                                >  6:13140/1‑149 (MQ=255)
                                                                aGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcag                                                                               >  1:113513/1‑149 (MQ=255)
                                                                aGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcag                                                                               <  4:112415/149‑1 (MQ=255)
                                                                    ccGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      <  2:12159/122‑1 (MQ=255)
                                                                    ccGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                      >  1:12159/1‑122 (MQ=255)
                                                                     cGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                               <  7:82156/112‑1 (MQ=255)
                                                                     cGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGCGTCTCCATg                                                                                                               >  8:82156/1‑112 (MQ=255)
                                                                        gcgcATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                        >  5:294857/1‑148 (MQ=255)
                                                                         cgcATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGa                                                                      <  2:113513/149‑1 (MQ=255)
                                                                               gC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAggg                                                                 <  6:294857/148‑1 (MQ=255)
                                                                                c‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                        <  1:291170/140‑1 (MQ=255)
                                                                                c‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                        >  2:291170/1‑140 (MQ=255)
                                                                                     ataGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCacg                                                                                                                        >  1:192046/1‑86 (MQ=38)
                                                                                     ataGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCacg                                                                                                                        <  2:192046/88‑3 (MQ=38)
                                                                                        gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGAATCCATGGTTTACTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                        >  1:263332/1‑149 (MQ=255)
                                                                                         aaCATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                      <  3:146992/86‑1 (MQ=255)
                                                                                         aaCATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                      >  4:146992/1‑86 (MQ=255)
                                                                                           cATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCgg                                                     >  1:87003/1‑149 (MQ=255)
                                                                                               ggCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAg                                                 >  8:17254/1‑149 (MQ=255)
                                                                                                gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                          >  6:47340/1‑91 (MQ=255)
                                                                                                gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                          <  5:47340/91‑1 (MQ=255)
                                                                                                gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGt                                                >  1:48334/1‑149 (MQ=255)
                                                                                                gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGt                                                >  1:5081/1‑149 (MQ=255)
                                                                                                          ggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgc                                      >  3:141123/1‑149 (MQ=255)
                                                                                                                gATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGa                                <  3:250403/149‑1 (MQ=255)
                                                                                                                gATGCCCAGTGGCACGCCCATCACCACACAAACCACCCTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGa                                <  6:250124/149‑1 (MQ=255)
                                                                                                                 aTGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                               <  2:5081/149‑1 (MQ=255)
                                                                                                                 aTGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                               <  7:17254/149‑1 (MQ=255)
                                                                                                                     ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAAAGCGGTCAGTGCCCACTTGCGCTGGATGaaa                           >  5:127225/1‑149 (MQ=255)
                                                                                                                                       ccacACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAAt         <  2:48334/149‑1 (MQ=255)
                                                                                                                                        cacacaTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATa        <  4:141123/149‑1 (MQ=255)
                                                                                                                                           acaTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAggg     <  5:13140/149‑1 (MQ=255)
                                                                                                                                             aTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGc    >  4:293155/1‑148 (MQ=255)
                                                                                                                                              tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGa  >  6:162055/1‑149 (MQ=255)
                                                                                                                                                  accGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAg       >  6:158834/1‑140 (MQ=255)
                                                                                                                                                  accGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAg       <  5:158834/140‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
CAGGCCGAACAGGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGA  >  NC_002947/354719‑355008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑