Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,523,896 G→C 7.0% G582A (GGC→GCC)  idh → isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,523,8960GC 7.0% 181.1 / 2.9 57G582A (GGC→GCC) idhisocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (22/31);  new base C (2/2);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

ATCCGCGAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTACGACAACCCGCGCGC  >  NC_002947/4523748‑4524031
                                                                                                                                                     |                                                                                                                                       
aTCCGCGAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAAccgg                                                                                                                                         >  6:319895/1‑149 (MQ=255)
 tCCGCGAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAAccggc                                                                                                                                        <  7:73767/149‑1 (MQ=255)
    gcgAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCAAAAccggcgcc                                                                                                                                     <  4:156888/149‑1 (MQ=255)
      gAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAAccggcgccgg                                                                                                                                   >  4:101200/1‑149 (MQ=255)
           cAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGtt                                                                                                                              <  8:286138/149‑1 (MQ=255)
           cAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGtt                                                                                                                              <  3:709/149‑1 (MQ=255)
                acacCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCt                                                                                                                         >  7:44486/1‑149 (MQ=255)
                 cacCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTc                                                                                                                        <  3:82331/149‑1 (MQ=255)
                  acCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTcc                                                                                                                       >  2:247550/1‑149 (MQ=255)
                   ccATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTcc                                                                                                                       >  3:204104/1‑148 (MQ=255)
                     aTCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGaa                                                                                                                    <  3:310737/149‑1 (MQ=255)
                      tctcGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAg                                                                                                                   >  1:207728/1‑149 (MQ=255)
                          ggTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCAc                                                                                                                <  5:319895/148‑1 (MQ=255)
                          ggTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCAc                                                                                                                <  3:101200/148‑1 (MQ=255)
                            tGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTg                                                                                                             >  7:110560/1‑149 (MQ=255)
                             gACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTg                                                                                                             >  6:284905/1‑148 (MQ=255)
                                    aaCGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGCGCAACaaga                                                                                                     <  2:47539/149‑4 (MQ=255)
                                       gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         >  2:77646/1‑121 (MQ=255)
                                       gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         >  2:81844/1‑121 (MQ=255)
                                       gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         <  1:77646/126‑6 (MQ=255)
                                       gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         <  1:81844/126‑6 (MQ=255)
                                             cgcgACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGaa                                                                                              <  1:247550/147‑1 (MQ=255)
                                             cgcgACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAaga                                                                                            <  4:204104/149‑1 (MQ=255)
                                             cgcgACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAaga                                                                                            <  2:207728/149‑1 (MQ=255)
                                                gACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGaa                                                                                         <  8:44486/149‑1 (MQ=255)
                                                     ccTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTcc                                                                                    >  4:3743/1‑149 (MQ=255)
                                                     ccTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTcc                                                                                    >  7:156316/1‑149 (MQ=255)
                                                     ccTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTcc                                                                                    >  7:89411/1‑149 (MQ=255)
                                                      cTGGCCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGc                                                                                                                          <  6:326481/110‑1 (MQ=255)
                                                      cTGGCCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGc                                                                                                                          >  5:326481/1‑110 (MQ=255)
                                                         accgaccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTg                                                                                  <  7:102781/147‑1 (MQ=255)
                                                          ccgaccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGt                                                                               <  2:96444/149‑1 (MQ=255)
                                                           cgaccTGTTCCCGATCATGGAACTGGGCACTAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGtt                                                                              >  1:26066/1‑149 (MQ=255)
                                                           cgaccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTTCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGtt                                                                              <  5:307021/149‑1 (MQ=255)
                                                           cgaccTGTGCCCGATCATGGAACTGCGCACACTCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGGGCAACAGCTGGTTGAAGAGAACTTCCTGCGtt                                                                              <  4:36234/149‑1 (MQ=255)
                                                             accTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            <  8:110560/149‑1 (MQ=255)
                                                             accTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            >  4:80896/1‑149 (MQ=255)
                                                              ccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTggg                                                                           <  3:80896/149‑1 (MQ=255)
                                                                  ttCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTc                                                                       >  3:304763/1‑149 (MQ=255)
                                                                    cccGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGc                                                                     >  4:260811/1‑149 (MQ=255)
                                                                    cccGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGc                                                                     >  4:251608/1‑149 (MQ=255)
                                                                       gATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGaa                                                                                                                    >  8:267453/1‑99 (MQ=255)
                                                                       gATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGaa                                                                                                                    <  7:267453/99‑1 (MQ=255)
                                                                            tGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGaa                                                             >  5:309524/1‑149 (MQ=255)
                                                                            tGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGaa                                                             <  3:260811/149‑1 (MQ=255)
                                                                                    ggCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctgg                                                      <  2:26066/148‑1 (MQ=255)
                                                                                    ggCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctgg                                                      <  3:3743/148‑1 (MQ=255)
                                                                                         aaGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctggccctgg                                                <  1:253200/149‑1 (MQ=255)
                                                                                          aGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctggccctggc                                               <  8:156316/149‑1 (MQ=255)
                                                                                               cAAGATGCTGTCGATCGTGCCGCTGATGTACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCtt                                          >  1:181316/1‑149 (MQ=255)
                                                                                                      cTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGa                                   <  5:284905/149‑1 (MQ=255)
                                                                                                      cTGGCGATCGTGCGGCTGATGCACGGCGGTTGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGa                                   <  6:227609/149‑1 (MQ=255)
                                                                                                       tGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTGATTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTg                                     >  1:49071/1‑147 (MQ=255)
                                                                                                           gATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCAc                               <  3:251608/148‑1 (MQ=255)
                                                                                                                  ccGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCa                        <  4:304763/148‑1 (MQ=255)
                                                                                                                      tgatgaACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGc                         >  3:64471/1‑143 (MQ=255)
                                                                                                                      tgatgaACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGc                         <  4:64471/143‑1 (MQ=255)
                                                                                                                         tgaACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTAc                <  2:49071/149‑1 (MQ=255)
                                                                                                                                      ccTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            <  3:216526/76‑1 (MQ=255)
                                                                                                                                      ccTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            >  4:216526/1‑76 (MQ=255)
                                                                                                                                        tG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTACGACAACCCgcgcgc  >  2:49509/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                       
ATCCGCGAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTACGACAACCCGCGCGC  >  NC_002947/4523748‑4524031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑