Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,523,899	C→G	7.0%	A583G (GCC→GGC)	idh →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,523,899	0	C	G	7.0%	182.0 / 3.9	57	A583G (GCC→GGC)	idh	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (22/31);  new base G (2/2);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GCGAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTACGACAACCCGCGCGC  >  NC_002947/4523752‑4524031                                                                                                                                                     |                                                                                                                                     gcgAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCAAAAccggcgcc                                                                                                                                     <  4:156888/149‑1 (MQ=255)   gAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAAccggcgccgg                                                                                                                                   >  4:101200/1‑149 (MQ=255)        cAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGtt                                                                                                                              <  8:286138/149‑1 (MQ=255)        cAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGtt                                                                                                                              <  3:709/149‑1 (MQ=255)             acacCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCt                                                                                                                         >  7:44486/1‑149 (MQ=255)              cacCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTc                                                                                                                        <  3:82331/149‑1 (MQ=255)               acCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTcc                                                                                                                       >  2:247550/1‑149 (MQ=255)                ccATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTcc                                                                                                                       >  3:204104/1‑148 (MQ=255)                  aTCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGaa                                                                                                                    <  3:310737/149‑1 (MQ=255)                   tctcGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAg                                                                                                                   >  1:207728/1‑149 (MQ=255)                       ggTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCAc                                                                                                                <  3:101200/148‑1 (MQ=255)                       ggTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCAc                                                                                                                <  5:319895/148‑1 (MQ=255)                         tGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTg                                                                                                             >  7:110560/1‑149 (MQ=255)                          gACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTg                                                                                                             >  6:284905/1‑148 (MQ=255)                                 aaCGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGCGCAACaaga                                                                                                     <  2:47539/149‑4 (MQ=255)                                    gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         >  2:77646/1‑121 (MQ=255)                                    gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         >  2:81844/1‑121 (MQ=255)                                    gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         <  1:77646/126‑6 (MQ=255)                                    gTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGCCGGCGCCGGtttcgaa                                                                                                                         <  1:81844/126‑6 (MQ=255)                                          cgcgACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGaa                                                                                              <  1:247550/147‑1 (MQ=255)                                          cgcgACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAaga                                                                                            <  2:207728/149‑1 (MQ=255)                                          cgcgACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAaga                                                                                            <  4:204104/149‑1 (MQ=255)                                             gACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGaa                                                                                         <  8:44486/149‑1 (MQ=255)                                                  ccTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTcc                                                                                    >  7:89411/1‑149 (MQ=255)                                                  ccTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTcc                                                                                    >  7:156316/1‑149 (MQ=255)                                                  ccTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTcc                                                                                    >  4:3743/1‑149 (MQ=255)                                                   cTGGCCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGc                                                                                                                          >  5:326481/1‑110 (MQ=255)                                                   cTGGCCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGc                                                                                                                          <  6:326481/110‑1 (MQ=255)                                                      accgaccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTg                                                                                  <  7:102781/147‑1 (MQ=255)                                                       ccgaccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGt                                                                               <  2:96444/149‑1 (MQ=255)                                                        cgaccTGTTCCCGATCATGGAACTGGGCACTAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGtt                                                                              >  1:26066/1‑149 (MQ=255)                                                        cgaccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTTCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGtt                                                                              <  5:307021/149‑1 (MQ=255)                                                        cgaccTGTGCCCGATCATGGAACTGCGCACACTCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGGGCAACAGCTGGTTGAAGAGAACTTCCTGCGtt                                                                              <  4:36234/149‑1 (MQ=255)                                                          accTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            <  8:110560/149‑1 (MQ=255)                                                          accTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            >  4:80896/1‑149 (MQ=255)                                                           ccTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTggg                                                                           <  3:80896/149‑1 (MQ=255)                                                               ttCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTc                                                                       >  3:304763/1‑149 (MQ=255)                                                                 cccGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGc                                                                     >  4:260811/1‑149 (MQ=255)                                                                 cccGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGc                                                                     >  4:251608/1‑149 (MQ=255)                                                                    gATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGaa                                                                                                                    <  7:267453/99‑1 (MQ=255)                                                                    gATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGaa                                                                                                                    >  8:267453/1‑99 (MQ=255)                                                                         tGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGaa                                                             <  3:260811/149‑1 (MQ=255)                                                                         tGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGaa                                                             >  5:309524/1‑149 (MQ=255)                                                                                 ggCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctgg                                                      <  2:26066/148‑1 (MQ=255)                                                                                 ggCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctgg                                                      <  3:3743/148‑1 (MQ=255)                                                                                      aaGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctggccctgg                                                <  1:253200/149‑1 (MQ=255)                                                                                       aGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTcctggccctggc                                               <  8:156316/149‑1 (MQ=255)                                                                                            cAAGATGCTGTCGATCGTGCCGCTGATGTACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCtt                                          >  1:181316/1‑149 (MQ=255)                                                                                                   cTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGa                                   <  5:284905/149‑1 (MQ=255)                                                                                                   cTGGCGATCGTGCGGCTGATGCACGGCGGTTGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGa                                   <  6:227609/149‑1 (MQ=255)                                                                                                    tGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTGATTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTg                                     >  1:49071/1‑147 (MQ=255)                                                                                                        gATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCAc                               <  3:251608/148‑1 (MQ=255)                                                                                                               ccGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCa                        <  4:304763/148‑1 (MQ=255)                                                                                                                   tgatgaACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGc                         <  4:64471/143‑1 (MQ=255)                                                                                                                   tgatgaACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGc                         >  3:64471/1‑143 (MQ=255)                                                                                                                      tgaACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTAc                <  2:49071/149‑1 (MQ=255)                                                                                                                                   ccTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            <  3:216526/76‑1 (MQ=255)                                                                                                                                   ccTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTgg                                                                            >  4:216526/1‑76 (MQ=255)                                                                                                                                     tG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTACGACAACCCgcgcgc  >  2:49509/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                     GCGAAGGCAAGGACACCATCTCGGTGACCGGCAACGTGCTGCGCGACTACCTGACCGACCTGTTCCCGATCATGGAACTGGGCACAAGCGCCAAGATGCTGTCGATCGTGCCGCTGATGAACGGCGGTGGCCTG‑TTCGAAACCGGCGCCGGCGGTTCGGCTCCGAAGCACGTGCAACAGCTGGTTGAAGAGAACTTCCTGCGTTGGGACTCGCTGGGTGAATTCCTGGCCCTGGCCGCTTCCCTGGAGCACCTGGGCAACACCTACGACAACCCGCGCGC  >  NC_002947/4523752‑4524031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑