Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,528,029 G→C 8.9% E359Q (GAG→CAG)  purB → adenylosuccinate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,528,0290GC 8.9% 123.9 / 2.2 43E359Q (GAG→CAG) purBadenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base C (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGGCAACCTGGGTATCGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACGAGAAGCTCAAAG  >  NC_002947/4527887‑4528176
                                                                                                                                              |                                                                                                                                                    
cGAAGGCAACCTGGGTATCGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCTATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGc                                                                                                                                                  <  7:250436/147‑1 (MQ=255)
cGAAGGCAACCTGGGTATCGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCa                                                                                                                                                <  4:283427/149‑1 (MQ=255)
         ccTGGGTATCGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAg                                                                                                                                       <  4:166732/149‑1 (MQ=255)
                  cGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTTCAGGCTGGCCTCGAAGGcgatg                                                                                                                                 >  8:60713/1‑142 (MQ=255)
                  cGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTTCAGGCTGGCCTCGAAGGcgatg                                                                                                                                 <  7:60713/146‑5 (MQ=255)
                   gCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGc                                                                                                                               >  3:273790/1‑147 (MQ=255)
                          cgcTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGaa                                                                                                                      <  3:110008/149‑1 (MQ=255)
                            cTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCAAACCGTAATCGCCTTCGACGCCAGCCTCAAACGAATCCGCAAACTGGaat                                                                                                                     >  8:241333/1‑147 (MQ=255)
                               ttCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTTCAGGCTGGCCTCGAAGGcgatga                                                                                                                                >  3:295685/1‑129 (MQ=255)
                               ttCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTTCAGGCTGGCCTCGAAGGcgatga                                                                                                                                <  4:295685/134‑6 (MQ=255)
                               ttCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCaa                                                                                                                 <  3:18885/149‑1 (MQ=255)
                                  cAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCAt                                                                                                                                   <  2:207938/128‑1 (MQ=255)
                                  cAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCAt                                                                                                                                   >  1:207938/1‑128 (MQ=255)
                                       ccTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTc                                                                                                         <  2:122116/149‑1 (MQ=255)
                                                      gCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGAcc                                                                                          >  2:219780/1‑149 (MQ=255)
                                                               gcgcTTGCATCGTGACCTGACCGACTACACCGTGCTGCGCAAAATTGGCGTGGGCTTTGCCCAAAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGa                                                                                                                       <  1:315522/111‑1 (MQ=255)
                                                               gcgcTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTTGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTgg                                                                                       <  4:226116/143‑1 (MQ=255)
                                                               gcgcTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGa                                                                                                                                                     >  8:183847/1‑81 (MQ=255)
                                                               gcgcTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGa                                                                                                                                                     <  7:183847/81‑1 (MQ=255)
                                                                cgcTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCct‑g                                                                               >  5:226084/1‑149 (MQ=255)
                                                                 gcTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCct‑gc                                                                              <  4:273790/149‑1 (MQ=255)
                                                                   tGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGACTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCct‑gctg                                                                            >  2:234477/1‑149 (MQ=255)
                                                                         cGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCct‑gctg                                                                            <  7:303135/143‑1 (MQ=255)
                                                                         cGTGACATGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACCGCGTAATCGCCTTCGAGGCCAGCCTGAAACGGCTCGGCAAGCTGGAAGTCAACGAAGCTCGCCTCTACGCCGCCCTGGCCGCct‑ggag                                                                            >  8:303135/1‑140 (MQ=255)
                                                                          gTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGt                                                                      <  6:226084/148‑1 (MQ=255)
                                                                           tgacctgaccGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAACGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGc                                                                    >  4:239368/1‑149 (MQ=255)
                                                                              cctgaccGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTgg                                                                 <  6:269506/149‑1 (MQ=255)
                                                                                    cGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGgccgagc                                                           <  3:239368/149‑1 (MQ=255)
                                                                                    cGACTCCACCGTGCTGCGCAACCTGGGCGTGGACTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGgccgagc                                                           <  1:234477/149‑1 (MQ=255)
                                                                                               tgctgcGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGAcc                                                >  7:41203/1‑149 (MQ=255)
                                                                                                     gcAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCTCGCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGAt                                           <  1:219780/149‑1 (MQ=255)
                                                                                                             ggCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGcgccgc                                    <  8:41203/147‑1 (MQ=255)
                                                                                                                   ggCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACa                             <  6:214836/148‑1 (MQ=255)
                                                                                                                            cacaGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAAccc                   >  5:71433/1‑149 (MQ=255)
                                                                                                                             acaGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGaaaa                      >  2:52005/1‑145 (MQ=255)
                                                                                                                             acaGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGaaaa                      <  1:52005/145‑1 (MQ=255)
                                                                                                                             acaGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAAcccc                  >  5:39882/1‑149 (MQ=255)
                                                                                                                                     aTCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACgag            >  8:267931/1‑147 (MQ=255)
                                                                                                                                        gCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCTGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACGAGAAGCt       >  3:29375/1‑149 (MQ=255)
                                                                                                                                          cTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATc                           <  6:60996/127‑1 (MQ=255)
                                                                                                                                          cTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATc                           >  5:60996/1‑127 (MQ=255)
                                                                                                                                           ttCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACgaga           >  7:5534/1‑142 (MQ=255)
                                                                                                                                           ttCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACgaga           <  8:5534/142‑1 (MQ=255)
                                                                                                                                              gAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAATTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACGAGAAGCTCAAag  <  7:267931/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CGAAGGCAACCTGGGTATCGCCAACGCGCTGTTCCAGCACCTGGCCAGCAAGCTGCCGATCTCGCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGCAACCTGGGCGTGGGCTTTGCCCACAGCGTCATCGCCTTCGAGGCCAGCCTGAAAGGCATCGGCAAGCTGGAAGTCAACGAAGCTCGCATCGCCGCCGACCTGGACGCCT‑GCTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTTCAACATCGAAAACCCCTACGAGAAGCTCAAAG  >  NC_002947/4527887‑4528176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑