Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 354,873 A→C 7.7% F100C (TTC→TGC)  cbcW ← choline/betaine/carnitine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947354,8730AC 7.7% 155.8 / 3.9 52F100C (TTC→TGC) cbcWcholine/betaine/carnitine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base C (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACG  >  NC_002947/354732‑355020
                                                                                                                                              |                                                                                                                                                   
ggcatggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGAc                                                                                                                                              <  3:210155/144‑1 (MQ=255)
          ggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAg                                                                                                                                     <  1:304241/148‑1 (MQ=255)
               aGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGtctc                                                                                                                               <  5:190036/149‑1 (MQ=255)
                 gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGtggt                                                                                                                               <  3:284064/147‑4 (MQ=255)
                 gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGtggt                                                                                                                               >  4:284064/1‑144 (MQ=255)
                    gACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                                           <  5:287395/148‑1 (MQ=255)
                         aGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                      >  1:62776/1‑149 (MQ=255)
                            tGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  <  8:19854/149‑1 (MQ=255)
                            tGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  <  5:99424/149‑1 (MQ=255)
                            tGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  <  2:62776/149‑1 (MQ=255)
                              ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  >  3:287317/1‑147 (MQ=255)
                              ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  <  4:287317/147‑1 (MQ=255)
                                cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAg                                                                                                               >  3:112415/1‑148 (MQ=255)
                                         cATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGt                                                                                                     >  4:250403/1‑149 (MQ=255)
                                                  gAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGAACACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATca                                                                                            >  6:13140/1‑149 (MQ=255)
                                                   aGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcag                                                                                           >  1:113513/1‑149 (MQ=255)
                                                   aGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcag                                                                                           <  4:112415/149‑1 (MQ=255)
                                                       ccGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  >  1:12159/1‑122 (MQ=255)
                                                       ccGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                  <  2:12159/122‑1 (MQ=255)
                                                        cGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                                           <  7:82156/112‑1 (MQ=255)
                                                        cGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGCGTCTCCATg                                                                                                                           >  8:82156/1‑112 (MQ=255)
                                                           gcgcATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                    >  5:294857/1‑148 (MQ=255)
                                                            cgcATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGa                                                                                  <  2:113513/149‑1 (MQ=255)
                                                                  gC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAggg                                                                             <  6:294857/148‑1 (MQ=255)
                                                                   c‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                    <  1:291170/140‑1 (MQ=255)
                                                                   c‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                    >  2:291170/1‑140 (MQ=255)
                                                                        ataGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCacg                                                                                                                                    >  1:192046/1‑86 (MQ=38)
                                                                        ataGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCacg                                                                                                                                    <  2:192046/88‑3 (MQ=38)
                                                                           gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGAATCCATGGTTTACTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                                    >  1:263332/1‑149 (MQ=255)
                                                                            aaCATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                                  >  4:146992/1‑86 (MQ=255)
                                                                            aaCATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                                  <  3:146992/86‑1 (MQ=255)
                                                                              cATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCgg                                                                 >  1:87003/1‑149 (MQ=255)
                                                                                  ggCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAg                                                             >  8:17254/1‑149 (MQ=255)
                                                                                   gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                      >  6:47340/1‑91 (MQ=255)
                                                                                   gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                      <  5:47340/91‑1 (MQ=255)
                                                                                   gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGt                                                            >  1:48334/1‑149 (MQ=255)
                                                                                   gCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGt                                                            >  1:5081/1‑149 (MQ=255)
                                                                                             ggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgc                                                  >  3:141123/1‑149 (MQ=255)
                                                                                                   gATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGa                                            <  3:250403/149‑1 (MQ=255)
                                                                                                   gATGCCCAGTGGCACGCCCATCACCACACAAACCACCCTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGa                                            <  6:250124/149‑1 (MQ=255)
                                                                                                    aTGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                                           <  2:5081/149‑1 (MQ=255)
                                                                                                    aTGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                                           <  7:17254/149‑1 (MQ=255)
                                                                                                        ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAAAGCGGTCAGTGCCCACTTGCGCTGGATGaaa                                       >  5:127225/1‑149 (MQ=255)
                                                                                                                          ccacACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAAt                     <  2:48334/149‑1 (MQ=255)
                                                                                                                           cacacaTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATa                    <  4:141123/149‑1 (MQ=255)
                                                                                                                              acaTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAggg                 <  5:13140/149‑1 (MQ=255)
                                                                                                                                aTACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGc                >  4:293155/1‑148 (MQ=255)
                                                                                                                                 tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGa              >  6:162055/1‑149 (MQ=255)
                                                                                                                                     accGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAg                   >  6:158834/1‑140 (MQ=255)
                                                                                                                                     accGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAg                   <  5:158834/140‑1 (MQ=255)
                                                                                                                                           gCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaacgaa    <  6:67699/149‑1 (MQ=255)
                                                                                                                                             gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGaacgaacg  >  7:235767/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGC‑GGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACG  >  NC_002947/354732‑355020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑