| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,530,025 | T→A | 13.8% | intergenic (+31/‑137) | PP_4018 → / → topB | acetyltransferase/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,530,025 | 0 | T | A | 13.8% | 75.1 / 4.7 | 35 | intergenic (+31/‑137) | PP_4018/topB | acetyltransferase/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base A (3/2); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01 | |||||||||||
CGCCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATG > NC_002947/4529880‑4530164 | cgcCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTAc < 6:287140/149‑1 (MQ=255) cccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTAccc < 1:196259/149‑1 (MQ=255) cccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGAGCCGGCCTCTTCGCGGGTAAAccc > 6:102986/1‑149 (MQ=255) ccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCc‑g > 8:41232/1‑149 (MQ=255) ccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCc‑g > 2:102253/1‑149 (MQ=255) gTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCGCCTGAGCCGGCCTCTTCGCGGGTAAACCC‑GCGaa < 4:134213/149‑1 (MQ=39) tggacgccacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAag < 8:154056/146‑1 (MQ=255) gCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAaga < 1:102253/149‑1 (MQ=255) acgccacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGAATCGCGCGCCTGATCCATCACCTGCACTGGCCTAATAGCGGGTTTACCC‑CCGAAAAgg > 3:7633/1‑149 (MQ=255) gccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTAGCGGGTTTACCC‑GCGAAGAGGCCGGCt > 3:166529/1‑148 (MQ=255) tGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGAGCCGGCCTCTTCGCGGGTAAACCC‑GCGAAGAGGCCAGTGCAGGTGat < 5:102986/147‑1 (MQ=25) cGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACAt > 3:229755/1‑149 (MQ=255) cTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATccc > 7:185553/1‑149 (MQ=255) aGCGAGTATTTCCTCGAAGCTGTCATCCCGCATGTCGACATGGTGCGCGACGCGCGCGCCTTATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGt < 2:99871/149‑1 (MQ=255) cGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTa > 3:284174/1‑148 (MQ=255) gAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGAt > 7:276518/1‑148 (MQ=255) aaGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGt < 7:240447/133‑1 (MQ=255) aaGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGt > 8:240447/1‑133 (MQ=255) ggCACCCCGCTTGCCGACTTGGGGCGCGACTCGCGCGCCTTACCCACCACCTCCCCTGGCCTCTGCGCGGTTTTCCCC‑GCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACtt < 4:229755/149‑1 (MQ=255) cgttctGTCGACTTGGTGCGCGCTGCGGGCGCCTGATACATCCCCTGCACTGGCCTCTTCGC‑GGTTTACCCGGTTAAGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCctct < 4:166529/144‑1 (MQ=255) tCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGcccc > 3:101943/1‑149 (MQ=255) aCATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGc < 8:185553/149‑1 (MQ=255) atcgcgcgcCTCGCGCCCCTGATCCATCACCTGCAGTGGCCTCTTCGCGGGTTAACCC‑GCTAAGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc < 2:107640/146‑1 (MQ=255) gTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGTTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc < 4:284174/148‑1 (MQ=255) gcgACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATGCTTGCCAGATAAAACTTGCCACAGCGCCCCCGCTTgc > 1:107640/1‑145 (MQ=255) gcgcgcCTGATCCATCACCTGCACTGCCCTCTTCGCGGGTTTACCC‑GCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATgg < 1:128454/148‑1 (MQ=255) tCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATACAT‑CCAGATAAAACTTTCCTCTGCGCCCCCGCTTGCGCATACTATGGCcccccccccc > 7:167295/1‑149 (MQ=255) ccTGCACTGGCCTCTTCCGGGGTTAACCC‑GGTATGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCTCATCCTATGGCCCACCACCCCGATGAAg < 7:41232/149‑1 (MQ=255) ctctTCGCGGGTAAACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGc > 7:183358/1‑148 (MQ=255) tctTCGCGGGTAAACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGc > 6:298905/1‑147 (MQ=255) ttCGCGTGTTTACCC‑GCGAAGACGCCGTCTCAGTCGATATACATCCCCGCTGACAAAAAACCAGTACATCCAT‑ACAGATAAAAATTGAATCTCAGCCAACGCTTACGCATCATATGGCAAACCACACCTATGAATAATCCCCGGCCATg > 6:306920/1‑149 (MQ=255) ttCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg > 1:142785/1‑149 (MQ=255) ttCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg > 8:235324/1‑149 (MQ=255) gTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCg > 4:153561/1‑122 (MQ=255) gTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCg < 3:153561/122‑1 (MQ=255) | CGCCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCC‑GCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCAT‑CCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATG > NC_002947/4529880‑4530164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |