Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,541,837 A→T 6.7% Q185L (CAG→CTG) ‡ nhaB → Na(+)/H(+) antiporter NhaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,541,8370AT 6.7% 177.3 / 3.0 60Q185L (CAG→CTG) ‡nhaBNa(+)/H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base A (27/29);  new base T (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCAC  >  NC_002947/4541691‑4541979
                                                                                                                                                  |                                                                                                                                              
cGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAg                                                                                                                                               <  7:15976/148‑1 (MQ=255)
       tCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGc                                                                                                                                       >  6:189748/1‑149 (MQ=255)
          gCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCa                                                                                                                                    >  1:156839/1‑149 (MQ=255)
                   tCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCg                                                                                                                           <  4:225110/149‑1 (MQ=255)
                   tCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCg                                                                                                                           >  1:63795/1‑149 (MQ=255)
                     cgcgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCATAGCTGCGGTCGGCTTCTACGCGGTGTACCAGCTCGTCGCTTCAGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCgg                                                                                                                          <  6:292526/148‑1 (MQ=255)
                     cgcgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGTGAGCGACTTGCTGGTCGCTCTccagtgc                                                                                                                             <  8:98751/145‑6 (MQ=255)
                     cgcgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGTGAGCGACTTGCTGGTCGCTCTccagtgc                                                                                                                             >  7:98751/1‑140 (MQ=255)
                      gcgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACTTGCTGGTCGCTctc                                                                                                                                   <  1:149115/138‑3 (MQ=255)
                      gcgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACTTGCTGGTCGCTctc                                                                                                                                   >  2:149115/1‑136 (MQ=255)
                       cgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGaa                                                                                                                       <  2:156839/149‑1 (MQ=255)
                       cgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGaa                                                                                                                       <  3:91740/149‑1 (MQ=255)
                         tttCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAg                                                                                                                      <  6:325744/148‑1 (MQ=255)
                         tttCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAg                                                                                                                      >  5:325744/1‑148 (MQ=255)
                            ctcGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCt                                                                                                                  >  4:231472/1‑149 (MQ=255)
                            ctcGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCt                                                                                                                  <  6:290108/149‑1 (MQ=255)
                               gATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCg                                                                                                                <  1:235068/148‑1 (MQ=255)
                                            tCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTc                                                                                                                                         >  4:39970/1‑110 (MQ=255)
                                            tCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTc                                                                                                                                         <  3:39970/110‑1 (MQ=255)
                                            tCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGcc                                                                                                  >  3:198279/1‑149 (MQ=255)
                                              aCGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCtt                                                                                                >  6:291646/1‑149 (MQ=255)
                                                    gTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCg                                                                                          >  5:146923/1‑149 (MQ=255)
                                                    gTGAGCCTCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGCGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCg                                                                                          <  8:155035/149‑1 (MQ=255)
                                                     tGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGt                                                                                         <  6:146923/149‑1 (MQ=255)
                                                       atcatcAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTa                                                                                        <  5:291646/148‑1 (MQ=255)
                                                          atcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCAACGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCct                                                                                    >  8:85625/1‑149 (MQ=255)
                                                           tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctg                                                                                   >  1:85942/1‑149 (MQ=255)
                                                             aGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctgc                                                                                  <  6:320084/148‑1 (MQ=255)
                                                              gcgcTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctgctg                                                                                <  4:291796/149‑1 (MQ=255)
                                                                       tCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGgc                                                                       <  2:261254/149‑1 (MQ=255)
                                                                            ttCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                  >  2:50556/1‑149 (MQ=255)
                                                                            ttCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                  >  5:270462/1‑149 (MQ=255)
                                                                              cgaCGCGGTGGTACACCGCGTAGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTgg                                                                >  4:148501/3‑149 (MQ=255)
                                                                              cTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                  <  3:66556/147‑1 (MQ=255)
                                                                              cTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                  <  5:49291/147‑1 (MQ=255)
                                                                               tACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCCTGGCGCTGTggg                                                               >  8:38500/1‑149 (MQ=255)
                                                                                 cgcgGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTgg                                                                <  4:198279/146‑1 (MQ=255)
                                                                                 cgcgGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTa                                                             >  3:51785/1‑149 (MQ=255)
                                                                                          ccaccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTTGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGAGCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTgg                                                    >  1:40074/1‑149 (MQ=255)
                                                                                          ccaccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTgg                                                    <  7:5428/149‑1 (MQ=255)
                                                                                           caccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTggg                                                   >  1:56798/1‑149 (MQ=255)
                                                                                            accGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggc                                                  >  2:93287/1‑149 (MQ=255)
                                                                                             ccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTCGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCAGCTCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggcg                                                 >  3:266810/1‑149 (MQ=255)
                                                                                                cgtcgcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGgcggcg                                              <  1:93287/149‑1 (MQ=255)
                                                                                                 gtcgcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGt                                             <  2:63795/149‑1 (MQ=255)
                                                                                                    gcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGttt                                           <  2:56798/148‑1 (MQ=255)
                                                                                                             gCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGTAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTtggt                                 <  1:205299/149‑1 (MQ=255)
                                                                                                               cAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTtggtgg                               <  5:37834/149‑1 (MQ=255)
                                                                                                                     gcgcgAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAACACCTCGACCAGTTCCGCGCCTTACTCAGTAGCCTGCTGATGCATGGAGCTGTGGGTACCGCCCTGCGCGGCGTTTGCACGTTGGTCGGCGAGc                         >  2:321795/1‑149 (MQ=255)
                                                                                                                             aCAGCGCACTGGAGAGCGACCAGCAAGTCTCTCACCTGCACGGGGAAGACCTCGACCAGTTCCGCGCCTTGGTGCGTAGCCTGGTGATTCATGGCGCTGTGGGTACCGCCCTGGGCGGGGTTTGCACGTTGGTGGTCGAGCCGCAGAAc                 <  2:40074/149‑1 (MQ=255)
                                                                                                                                    aCTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTa                                                                                        <  7:77285/71‑1 (MQ=255)
                                                                                                                                    aCTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTa                                                                                        >  8:77285/1‑71 (MQ=255)
                                                                                                                                      tGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGTCCTCGAGCAGTTCCGCGCCTTGCTGGGTAGCGTGCTGATGGATGGCGCTGTGGGTACGGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATc        <  1:321795/149‑1 (MQ=255)
                                                                                                                                        gagaGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACctgc             >  4:14813/1‑142 (MQ=255)
                                                                                                                                        gagaGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACctgc             <  3:14813/142‑1 (MQ=255)
                                                                                                                                        gagaGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCgg      >  6:51964/1‑149 (MQ=255)
                                                                                                                                          gagCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGc     >  2:47923/1‑148 (MQ=255)
                                                                                                                                               aCCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCa   <  6:46858/145‑1 (MQ=255)
                                                                                                                                               aCCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCa   >  5:46858/1‑145 (MQ=255)
                                                                                                                                               aCCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCAc  >  7:74627/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCAC  >  NC_002947/4541691‑4541979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑