Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,551,474 G→C 7.6% Q281H (CAG→CAC)  pydA → NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,551,4740GC 7.6% 154.3 / 2.9 52Q281H (CAG→CAC) pydANADP‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base G (20/28);  new base C (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GTTCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATACCTG  >  NC_002947/4551326‑4551621
                                                                                                                                                    |                                                                                                                                                   
gTTCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAg                                                                                                                                                     >  2:183897/1‑149 (MQ=255)
gTTCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAg                                                                                                                                                     >  6:282493/1‑149 (MQ=255)
    ggCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGgtgt                                                                                                                                                 >  3:29143/1‑149 (MQ=255)
    ggCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGgtgt                                                                                                                                                 <  4:242734/149‑1 (MQ=255)
       aGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCa                                                                                                                                              >  1:153169/1‑149 (MQ=255)
           aaGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCAcggc                                                                                                                                          <  7:236242/149‑1 (MQ=255)
           aaGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCACACCTGCAcggc                                                                                                                                          >  1:227957/1‑149 (MQ=255)
             gCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCAcggcgg                                                                                                                                        <  3:5330/149‑1 (MQ=255)
              ccGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCACACCTGCAcggcgcc                                                                                                                                       <  2:227957/149‑3 (MQ=255)
                  ttGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGAtg                                                                                                                                   >  3:54140/1‑149 (MQ=255)
                     ccTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGAtgctg                                                                                                                                <  1:25278/149‑1 (MQ=255)
                      cTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGAtgctg                                                                                                                                >  7:120994/1‑148 (MQ=255)
                         gAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCAt                                                                                                                             <  6:59627/148‑1 (MQ=255)
                           aCATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATgg                                                                                                                           <  3:23879/148‑1 (MQ=255)
                             aTGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGcgt                                                                                                                        >  6:198859/1‑147 (MQ=255)
                                gtggCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTcc                                                                                                                      <  4:29143/148‑1 (MQ=255)
                                 tggCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTcc                                                                                                                      <  3:155135/147‑1 (MQ=255)
                                 tggCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTcc                                                                                                                      >  4:155135/1‑147 (MQ=255)
                                     ggAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTAtcg                                                                                                                <  4:181886/149‑1 (MQ=255)
                                       aaaTCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTAtcgtc                                                                                                              >  8:147256/1‑149 (MQ=255)
                                           cGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTAtcgtc                                                                                                              >  3:189576/1‑145 (MQ=255)
                                           cGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTAtcgtc                                                                                                              <  4:189576/145‑1 (MQ=255)
                                                    cccGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAg                                                                                                  >  7:224517/1‑148 (MQ=255)
                                                     ccGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGa                                                                                                >  3:88394/1‑149 (MQ=255)
                                                             gcgGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTgg                                                                                        <  8:11891/149‑1 (MQ=255)
                                                             gcgGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGGGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTgg                                                                                        <  3:150716/149‑1 (MQ=255)
                                                              cgGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGc                                                                                       <  7:147256/149‑1 (MQ=255)
                                                               ggggCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCa                                                                                      <  1:183897/149‑1 (MQ=255)
                                                                          aTTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCtggatgga                                                                           <  4:54140/149‑1 (MQ=255)
                                                                                  cATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATg                                                                   <  8:120994/149‑1 (MQ=255)
                                                                                     tGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATgg                                                                  <  1:318012/147‑1 (MQ=255)
                                                                                           cATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCa                                                          <  1:201870/149‑1 (MQ=255)
                                                                                                       gCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGt                                              >  4:50014/1‑149 (MQ=255)
                                                                                                          tGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTcc                                           >  1:304345/1‑149 (MQ=255)
                                                                                                             cgccgcCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCcgcg                                        <  8:224517/149‑1 (MQ=255)
                                                                                                              gccgccGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGc                                                            >  6:59149/1‑128 (MQ=255)
                                                                                                              gccgccGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGc                                                            <  5:59149/128‑1 (MQ=255)
                                                                                                                cgccgAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCggg                                      >  1:47286/1‑148 (MQ=255)
                                                                                                                      gTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGAATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCgg                               >  1:31920/1‑149 (MQ=255)
                                                                                                                         cATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTgg                            >  5:93437/1‑149 (MQ=255)
                                                                                                                          aTGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTggg                           <  3:50014/149‑1 (MQ=255)
                                                                                                                          aTGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTggg                           <  5:282493/149‑1 (MQ=255)
                                                                                                                          aTGGCCTTGGGCAGTGGCGCCGGGCAGGTGTGGACGGCGGCGATGGTGGATGGGGTACGGATGGTGGAGGACCTGCAGGACGGCCGGGCACCCGGGTTGGAACACCAAGGGGATGGCACGGTCGCAGCGTTCCCCGGGCCGGCGGTggg                           >  2:306029/1‑149 (MQ=255)
                                                                                                                           tGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTgggg                          <  5:198859/149‑1 (MQ=255)
                                                                                                                               cTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATa                      >  5:259397/1‑149 (MQ=255)
                                                                                                                                ttGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATac                     >  7:101040/1‑149 (MQ=255)
                                                                                                                                  gggCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATacc                    <  6:151069/148‑1 (MQ=255)
                                                                                                                                       tcgccgcCGTGCACACCTGCACGGC‑GCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATaccac                  <  3:245528/140‑1 (MQ=255)
                                                                                                                                       tcgccgcCGTGCACACCTGCACGGC‑GCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATaccac                  >  4:245528/5‑144 (MQ=255)
                                                                                                                                       gTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGAc              <  1:306029/149‑1 (MQ=255)
                                                                                                                                        tGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACt             <  3:246750/149‑1 (MQ=255)
                                                                                                                                                 gCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGaa                                                  >  3:121376/1‑103 (MQ=255)
                                                                                                                                                 gCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGaa                                                  <  4:121376/103‑1 (MQ=255)
                                                                                                                                                   aGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATACCTg  <  2:47286/149‑1 (MQ=255)
                                                                                                                                                    ggTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATACCt   <  6:326899/147‑1 (MQ=255)
                                                                                                                                                    ggTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATACCt   >  5:326899/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACATGCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCGAAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATACCTG  >  NC_002947/4551326‑4551621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑