Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,585,555 A→T 11.8% I10N (ATT→AAT)  glgE ← alpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,585,5550AT11.8% 130.3 / 8.4 51I10N (ATT→AAT) glgEalpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (23/22);  new base T (4/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

CGTCGCTGTAGGCCTTGGTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACGGCAGCTG  >  NC_002947/4585421‑4585703
                                                                                                                                      |                                                                                                                                                     
cGTCGCTGTAGGCCTTGGTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCggg                                                                                                                                         >  2:113549/1‑149 (MQ=255)
cGTCGCTGTAGGCCTTGGTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGACCAGGCCATTAGCCTGGcccag                                                                                                                                          >  2:311632/1‑146 (MQ=255)
cGTCGCTGTAGGCCTTGGTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGACCAGGCCATTAGCCTGGcccag                                                                                                                                          <  1:311632/148‑3 (MQ=255)
             cTTGGTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCa                                                                                                                            >  1:113705/1‑149 (MQ=255)
                 gTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATgg                                                                                                                         <  8:41097/148‑1 (MQ=255)
                           ccACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTc                                                                                                               >  1:25132/1‑148 (MQ=255)
                            cacaGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCaa                                                                                                             <  4:59134/149‑1 (MQ=255)
                             acaGGCTGGCCTCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCaaa                                                                                                            <  8:261745/149‑1 (MQ=255)
                                ggCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAggg                                                                                                         <  1:113549/149‑1 (MQ=255)
                                       cGCTGACGGCCTTGGCGGTGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGtt                                                                                                   >  4:226997/1‑148 (MQ=255)
                                        gCTGACGGCCTTGGCGGTGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTc                                                                                                 <  3:226997/149‑1 (MQ=255)
                                        gCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTc                                                                                                 >  3:262611/1‑149 (MQ=255)
                                         cTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCg                                                                                                <  1:157856/149‑1 (MQ=255)
                                         cTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCg                                                                                                <  3:183014/149‑1 (MQ=255)
                                          tGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGt                                                                                               >  7:121183/1‑149 (MQ=255)
                                            gcGGCCTTGGCGGCGTATCTGCCGGCGTCGAGCACGGGTTGGGGGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCCGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGa                                                                                             <  6:200668/148‑1 (MQ=255)
                                              ggCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGa                                                                                             >  5:200668/1‑147 (MQ=255)
                                               gCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACAt                                                                                          >  2:181954/1‑149 (MQ=255)
                                                gcTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTc                                                                                                               <  3:228414/126‑1 (MQ=255)
                                                ccTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTc                                                                                                               >  4:228414/1‑127 (MQ=255)
                                                 cTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCg                                                                                        >  7:132983/1‑149 (MQ=255)
                                                     gcggcgAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATTGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCt                                                                                    <  8:121183/149‑1 (MQ=255)
                                                                 ccGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAg                                                                        <  3:308830/148‑1 (MQ=255)
                                                                     cGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCtgg                                                                   <  2:113705/149‑1 (MQ=255)
                                                                        cGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCtggtgg                                                                <  8:132983/149‑1 (MQ=255)
                                                                        cGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCtggtgg                                                                >  7:188884/1‑149 (MQ=255)
                                                                          aGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCg                                                              <  4:262611/149‑1 (MQ=255)
                                                                                       tgtCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCa                                                   >  5:224559/1‑147 (MQ=255)
                                                                                             cGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAggg                                                       <  4:3037/137‑1 (MQ=255)
                                                                                             cGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAggg                                                       >  3:3037/1‑137 (MQ=255)
                                                                                             cGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCCCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGcc                                              <  7:177102/146‑1 (MQ=255)
                                                                                             agATCACTATGCGTGGCGCCAACAGCGACTGGGCCAGGCTAATGGACTGGTACGGGTGATCGTTCGCCATGTGCACGCTTTCAAAGGTCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGcc                                              <  2:25132/145‑1 (MQ=255)
                                                                                               aTCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGca                                         >  8:20607/1‑149 (MQ=255)
                                                                                                   cGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAgg                                     >  4:48707/1‑149 (MQ=255)
                                                                                                    gATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGc                                    >  5:27947/1‑149 (MQ=255)
                                                                                                        cGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCgggg                                <  6:27947/149‑1 (MQ=255)
                                                                                                         gTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCggggg                               >  3:275450/1‑149 (MQ=255)
                                                                                                            gcgcCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGtt                            <  7:20607/149‑1 (MQ=255)
                                                                                                             cgcCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCg                                                              <  8:158572/114‑1 (MQ=255)
                                                                                                               ccAACAGCGCCTGGGCCAGGCTAATGGCCTCGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCa                          >  2:221233/1‑148 (MQ=255)
                                                                                                                       gcCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAAtt                  >  5:306088/1‑148 (MQ=255)
                                                                                                                         ccggaccAGGCCATTAGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTTCGCTCCCTCAGGCTGGTGGCGGTAAGGGtt                                                     <  1:24768/107‑1 (MQ=255)
                                                                                                                         ccggaccAGGCCATTAGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGtt                                                     >  2:24768/6‑111 (MQ=255)
                                                                                                                           gggCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGCCCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGc              >  4:143113/1‑148 (MQ=255)
                                                                                                                           ggaccAGGCCATTAGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGc              >  6:186665/4‑148 (MQ=255)
                                                                                                                           ggaccAGGCCATTAGCCTGGCCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGc              >  3:88987/4‑148 (MQ=255)
                                                                                                                               cAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACg         <  6:306088/149‑1 (MQ=255)
                                                                                                                                 ggCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACgg        >  3:12826/1‑148 (MQ=255)
                                                                                                                                 ggCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACgg        <  4:12826/148‑1 (MQ=255)
                                                                                                                                  gCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAAATGAGCGAACgg        >  2:132476/1‑147 (MQ=255)
                                                                                                                                    tAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACGGCAGc    >  7:41095/1‑149 (MQ=255)
                                                                                                                                      aTGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACGGCAGCTg  <  1:221233/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                     
CGTCGCTGTAGGCCTTGGTGCTGACGGCCACAGGCTGGCCGCTGACGGCCTTGGCGGCGAAGCTGCCGGCGTCGAGCACGGGTTGGGTGTCTTCGATCACGATGCGTGGCGCCAACAGCGCCTGGGCCAGGCTAATGGCCTGGTCCGGGTGATCGTTCGCCATGGGCACGCTTTCAAAGGGCTCGTTTCGTGACATCGACCTT‑GCTCCCTCAGGCTGGTGGCGGTAAGGGTTCAGAGCCGGGCACAGGCGGGGGGTTCAAAATAATTGAGCGAACGGCAGCTG  >  NC_002947/4585421‑4585703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑