Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 357,266 A→C 7.3% E204A (GAG→GCG)  tdcG‑I → L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947357,2660AC 7.3% 215.9 / 5.3 68E204A (GAG→GCG) tdcG‑IL‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base A (35/28);  new base C (3/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CAAGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCG  >  NC_002947/357121‑357412
                                                                                                                                                 |                                                                                                                                                   
cAAGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGa                                                                                                                                                  <  4:274865/149‑1 (MQ=255)
  aGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAg                                                                                                                                                <  6:85585/149‑1 (MQ=255)
      gccAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACAt                                                                                                                                            >  1:285572/1‑149 (MQ=255)
            ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCgcgc                                                                                                                                      <  7:225127/149‑1 (MQ=255)
            ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATACgcgc                                                                                                                                      >  6:150529/1‑149 (MQ=255)
             gtgtGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCCCt                                                                                                                                     >  7:225695/1‑149 (MQ=255)
                   tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                   >  8:220386/1‑144 (MQ=255)
                   tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                   <  7:220386/145‑2 (MQ=255)
                       tGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGcac                                                                                                                            >  4:194037/1‑148 (MQ=255)
                         cTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAg                                                                                                                         >  2:181160/1‑149 (MQ=255)
                          tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                              >  5:236233/1‑143 (MQ=255)
                          tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                              <  6:236233/143‑1 (MQ=255)
                                            gTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAg                                                                                                       >  2:246487/1‑148 (MQ=255)
                                                   ttCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAgg                                                                                                                                                   >  1:165664/1‑97 (MQ=255)
                                                   ttCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAgg                                                                                                                                                   <  2:165664/97‑1 (MQ=255)
                                                   ttCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                               >  8:29611/1‑149 (MQ=255)
                                                   ttCAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                               >  1:288084/1‑149 (MQ=255)
                                                   ttCAACAGAGCCGCAGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAAAGAGAATGTCTGGCGAAGCGATGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                               <  5:150529/149‑1 (MQ=255)
                                                     cAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTc                                                                                              <  2:288084/148‑1 (MQ=255)
                                                      aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                <  2:283213/145‑1 (MQ=255)
                                                      aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                >  1:283213/1‑145 (MQ=255)
                                                       aCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaa                                                                                            >  5:253615/1‑148 (MQ=255)
                                                         aGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaacaa                                                                                         >  7:37586/1‑149 (MQ=255)
                                                           cgccgcCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACg                                                                                       >  5:332638/1‑149 (MQ=255)
                                                            gccgccGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACgg                                                                                      >  3:101301/1‑149 (MQ=255)
                                                                ccgAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTc                                                                                  <  6:332638/149‑1 (MQ=255)
                                                                        ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                             <  7:253364/130‑1 (MQ=255)
                                                                        ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                             >  8:253364/1‑130 (MQ=255)
                                                                        ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTAc                                                                            >  4:113361/1‑147 (MQ=255)
                                                                         tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGaa                                                                                                                                                 >  1:106703/1‑77 (MQ=255)
                                                                         tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGaa                                                                                                                                                 <  2:106703/77‑1 (MQ=255)
                                                                         tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAg                                                                         >  7:280070/1‑149 (MQ=255)
                                                                                  gcaagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTa                                                               >  5:266058/1‑149 (MQ=255)
                                                                                   caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                >  4:278649/1‑132 (MQ=255)
                                                                                   caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                <  3:278649/132‑1 (MQ=255)
                                                                                      gcaaATCGACCTCAGGGTGTCGCAATTGATGATGGCGAAGGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACTAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGATGGC‑a                                                                    <  2:224775/140‑1 (MQ=255)
                                                                                      gcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑a                                                                    >  1:224775/1‑140 (MQ=255)
                                                                                           aCGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                      <  3:113361/149‑1 (MQ=255)
                                                                                                   aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                              <  1:181160/149‑1 (MQ=255)
                                                                                                   aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                              >  1:36676/1‑149 (MQ=255)
                                                                                                   aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCCGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAAc                                                <  2:285572/147‑1 (MQ=255)
                                                                                                         tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACTACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                         <  6:266058/148‑1 (MQ=255)
                                                                                                         tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                   <  2:32607/122‑1 (MQ=255)
                                                                                                         tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                   >  1:32607/1‑122 (MQ=255)
                                                                                                         tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                         <  6:253615/148‑1 (MQ=255)
                                                                                                           gCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCgccgcc                                      >  5:30591/1‑149 (MQ=255)
                                                                                                             aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                    >  6:107158/1‑149 (MQ=255)
                                                                                                             aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                    >  8:257481/1‑149 (MQ=255)
                                                                                                              aTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTg                                   >  8:164874/1‑149 (MQ=255)
                                                                                                                 gatgatGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                 <  3:194037/148‑1 (MQ=255)
                                                                                                                  atgatgGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgccgc                               >  5:87376/1‑149 (MQ=255)
                                                                                                                          cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                        >  1:245136/1‑148 (MQ=255)
                                                                                                                          cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                        >  4:165932/1‑148 (MQ=255)
                                                                                                                               agaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCg                   >  4:160591/1‑148 (MQ=255)
                                                                                                                                gaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                 >  8:295938/1‑149 (MQ=255)
                                                                                                                                  atgTCTGGCGCAGCGAGGTATCCATCCCCGCTGGCCTGGACAAGCTCTGGGAACCCATGCAGGAATGCGTCAACCACGGCCTCAATTACGCGGGCAACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                 <  1:246487/146‑1 (MQ=255)
                                                                                                                                  aGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCTGc               <  8:37586/149‑1 (MQ=255)
                                                                                                                                    gTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCt             <  3:160591/149‑1 (MQ=255)
                                                                                                                                     tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                 <  7:26407/144‑1 (MQ=255)
                                                                                                                                     tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                 >  8:26407/1‑144 (MQ=255)
                                                                                                                                       caggccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTg            >  4:239385/6‑147 (MQ=255)
                                                                                                                                         gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGc           <  3:88136/146‑1 (MQ=255)
                                                                                                                                         gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCa          <  8:225695/147‑1 (MQ=255)
                                                                                                                                         gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg         >  1:71694/1‑148 (MQ=255)
                                                                                                                                          gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                 >  7:37009/3‑123 (MQ=255)
                                                                                                                                          gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                 <  8:37009/121‑1 (MQ=255)
                                                                                                                                               cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGCGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg  <  4:101301/149‑1 (MQ=255)
                                                                                                                                               cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg  <  7:164874/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAAGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCG  >  NC_002947/357121‑357412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑