Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,587,698 G→C 6.3% S39S (TCC→TCG) ‡ PP_4063 ← long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,587,6980GC 6.3% 249.5 / 6.4 79S39S (TCC→TCG) ‡PP_4063long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (36/38);  new base C (3/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

GCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCA  >  NC_002947/4587556‑4587844
                                                                                                                                              |                                                                                                                                                  
gCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGTCCTGGTGGCGGGacac                                                                                                                                               >  7:71128/1‑148 (MQ=255)
gCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACAcc                                                                                                                                              >  8:112122/1‑149 (MQ=255)
 cAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGacac                                                                                                                                               <  1:261120/147‑1 (MQ=255)
  aaGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAg                                                                                                                                            <  8:100112/149‑1 (MQ=255)
   aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                           <  1:29761/149‑1 (MQ=255)
    gATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGTCCTGGTGGCGGGACACCAggg                                                                                                                                          <  8:71128/149‑1 (MQ=255)
    gATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAggg                                                                                                                                          <  1:51045/149‑1 (MQ=255)
      tGCACCACTGGGCGCAGTTGGGCGACCTGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGcc                                                                                                                                        <  8:78677/149‑1 (MQ=255)
        caccacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                           <  7:275320/144‑1 (MQ=255)
        caccacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                           >  8:275320/1‑144 (MQ=255)
         accacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCg                                                                                                                                     <  3:260200/149‑1 (MQ=255)
                  cgcAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCaa                                                                                                                            >  8:5499/1‑149 (MQ=255)
                       ttGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCg                                                                                                                       >  5:223609/1‑149 (MQ=255)
                         gggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAg                                                                                                                                            <  1:73355/126‑1 (MQ=255)
                         gggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAg                                                                                                                                            >  2:73355/1‑126 (MQ=255)
                           gCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGc                                                                                                                    >  5:269251/1‑148 (MQ=255)
                                  gATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                           >  5:310076/1‑118 (MQ=255)
                                  gATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAgg                                                                                                                                           <  6:310076/118‑1 (MQ=255)
                                      ccAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCAt                                                                                                         <  6:217729/148‑1 (MQ=255)
                                      ccAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATc                                                                                                        >  7:221109/1‑149 (MQ=255)
                                              gTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCt                                                                                                <  1:59991/149‑1 (MQ=255)
                                                 gCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGc                                                                                             <  6:223609/149‑1 (MQ=255)
                                                        ttCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGt                                                                                      >  7:172789/1‑149 (MQ=255)
                                                          cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGacac                                                                                                                                               <  6:316576/90‑1 (MQ=255)
                                                          cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGacac                                                                                                                                               >  5:316576/1‑90 (MQ=255)
                                                          cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTgg                                                                                              >  3:18786/1‑139 (MQ=255)
                                                          cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTgg                                                                                              <  4:18786/139‑1 (MQ=255)
                                                          cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCt                                                                                    >  5:194205/1‑149 (MQ=255)
                                                          cACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCt                                                                                    >  4:161282/1‑149 (MQ=255)
                                                           aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTg                                                                                   >  3:237447/1‑149 (MQ=255)
                                                            cGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTgg                                                                                  >  5:177698/1‑149 (MQ=255)
                                                               ccAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTc                                                                               <  6:177698/149‑1 (MQ=255)
                                                                 aGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAg                                                                             >  8:236402/1‑149 (MQ=255)
                                                                      gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAgg                                                                        <  7:5499/149‑1 (MQ=255)
                                                                       aTCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAggg                                                                       >  5:223713/1‑149 (MQ=255)
                                                                        tCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggt                                                                      >  7:147290/1‑149 (MQ=255)
                                                                           gggCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCggg                                                                                                                     >  8:223700/1‑99 (MQ=255)
                                                                           gggCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCggg                                                                                                                     <  7:223700/99‑1 (MQ=255)
                                                                           gggCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcgg                                                                   >  7:66023/1‑149 (MQ=255)
                                                                           gggCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcgg                                                                   <  7:112122/149‑1 (MQ=255)
                                                                            ggCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcggt                                                                  >  8:135809/1‑149 (MQ=255)
                                                                             gcgcGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcggtc                                                                 <  8:221109/149‑1 (MQ=255)
                                                                                 gggCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTgg                                                             <  5:61521/149‑1 (MQ=255)
                                                                                     gTATATTTCGACCTGTTCGGCAAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGAc                                                         >  1:189185/1‑149 (MQ=255)
                                                                                        tatTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACcgc                                                      <  3:161282/149‑1 (MQ=255)
                                                                                         atTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTc                                                                               <  6:145903/123‑1 (MQ=255)
                                                                                         atTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTc                                                                               >  5:145903/1‑123 (MQ=255)
                                                                                          tttCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAgg                                                                        >  2:65017/1‑129 (MQ=255)
                                                                                          tttCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAgg                                                                        <  1:65017/129‑1 (MQ=255)
                                                                                          tttCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                              >  5:28133/1‑139 (MQ=255)
                                                                                          tttCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                              <  6:28133/139‑1 (MQ=255)
                                                                                              gACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGtat                                                <  6:269251/149‑1 (MQ=255)
                                                                                              gACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTCGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGtat                                                <  5:277837/149‑1 (MQ=255)
                                                                                                        gCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                       >  4:68387/1‑148 (MQ=255)
                                                                                                          cAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                           >  3:310343/1‑142 (MQ=255)
                                                                                                          cAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                           <  4:310343/142‑1 (MQ=255)
                                                                                                          cAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAc                                    <  6:283293/149‑1 (MQ=255)
                                                                                                          cAACTGCCGCCAGCGGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAc                                    <  8:139109/149‑1 (MQ=255)
                                                                                                                ccgccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCAt                                <  2:189185/147‑1 (MQ=255)
                                                                                                                    cAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgtgt                          <  3:68387/149‑1 (MQ=255)
                                                                                                                      gCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTcc                        <  6:194205/149‑1 (MQ=255)
                                                                                                                        tGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGc                                                                            >  4:123242/1‑95 (MQ=255)
                                                                                                                        tGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGc                                                                            <  3:123242/95‑1 (MQ=255)
                                                                                                                        tGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGtat                                                <  1:216427/123‑1 (MQ=255)
                                                                                                                        tGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGtat                                                >  2:216427/1‑123 (MQ=255)
                                                                                                                          tAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttc                    >  1:149387/1‑149 (MQ=255)
                                                                                                                           aaCGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttc                    <  8:147290/148‑1 (MQ=255)
                                                                                                                            aCGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCcttctt                  >  4:16918/1‑149 (MQ=255)
                                                                                                                             cGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGCGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGTTGTCCcttcttt                 >  2:144533/1‑148 (MQ=255)
                                                                                                                              gCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgt                >  7:105154/1‑149 (MQ=255)
                                                                                                                               cAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgtg               <  7:135809/149‑1 (MQ=255)
                                                                                                                               cAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgtg               >  1:152764/1‑149 (MQ=255)
                                                                                                                                  gCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgtt            >  4:260873/1‑149 (MQ=255)
                                                                                                                                  gCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgtt            >  4:68580/1‑149 (MQ=255)
                                                                                                                                    ccTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCT‑GCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                       >  7:264822/1‑119 (MQ=255)
                                                                                                                                    ccTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCT‑GCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGtt                                       <  8:264822/119‑1 (MQ=255)
                                                                                                                                     cTGGTGGCGGGAGACCAGGGCCTCGGTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGGCCCTTCTTGTGgttgtt         <  3:93519/149‑1 (MQ=255)
                                                                                                                                       ggtggCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGtttt       >  3:174852/1‑149 (MQ=255)
                                                                                                                                            cGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCa  <  2:152764/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCA  >  NC_002947/4587556‑4587844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑