Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	357,270	A→T	7.6%	E205D (GAA→GAT)	tdcG‑I →	L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	357,270	0	A	T	7.6%	189.8 / 5.5	66	E205D (GAA→GAT)	tdcG‑I	L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base A (34/27);  new base T (3/2);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

AGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAA  >  NC_002947/357123‑357416                                                                                                                                                    |                                                                                                                                                    aGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAg                                                                                                                                                    <  6:85585/149‑1 (MQ=255)     gccAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACAt                                                                                                                                                >  1:285572/1‑149 (MQ=255)           ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCgcgc                                                                                                                                          <  7:225127/149‑1 (MQ=255)           ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATACgcgc                                                                                                                                          >  6:150529/1‑149 (MQ=255)            gtgtGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCCCt                                                                                                                                         >  7:225695/1‑149 (MQ=255)                  tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                       >  8:220386/1‑144 (MQ=255)                  tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                       <  7:220386/145‑2 (MQ=255)                      tGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGcac                                                                                                                                >  4:194037/1‑148 (MQ=255)                        cTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAg                                                                                                                             >  2:181160/1‑149 (MQ=255)                         tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                                  <  6:236233/143‑1 (MQ=255)                         tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                                  >  5:236233/1‑143 (MQ=255)                                           gTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAg                                                                                                           >  2:246487/1‑148 (MQ=255)                                                  ttCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                   >  8:29611/1‑149 (MQ=255)                                                  ttCAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                   >  1:288084/1‑149 (MQ=255)                                                  ttCAACAGAGCCGCAGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAAAGAGAATGTCTGGCGAAGCGATGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                   <  5:150529/149‑1 (MQ=255)                                                    cAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTc                                                                                                  <  2:288084/148‑1 (MQ=255)                                                     aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                    >  1:283213/1‑145 (MQ=255)                                                     aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                    <  2:283213/145‑1 (MQ=255)                                                      aCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaa                                                                                                >  5:253615/1‑148 (MQ=255)                                                        aGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaacaa                                                                                             >  7:37586/1‑149 (MQ=255)                                                          cgccgcCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACg                                                                                           >  5:332638/1‑149 (MQ=255)                                                           gccgccGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACgg                                                                                          >  3:101301/1‑149 (MQ=255)                                                               ccgAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTc                                                                                      <  6:332638/149‑1 (MQ=255)                                                                       ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                                 <  7:253364/130‑1 (MQ=255)                                                                       ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                                 >  8:253364/1‑130 (MQ=255)                                                                       ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTAc                                                                                >  4:113361/1‑147 (MQ=255)                                                                        tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGaa                                                                                                                                                     >  1:106703/1‑77 (MQ=255)                                                                        tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGaa                                                                                                                                                     <  2:106703/77‑1 (MQ=255)                                                                        tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAg                                                                             >  7:280070/1‑149 (MQ=255)                                                                                 gcaagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTa                                                                   >  5:266058/1‑149 (MQ=255)                                                                                  caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                    <  3:278649/132‑1 (MQ=255)                                                                                  caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                    >  4:278649/1‑132 (MQ=255)                                                                                     gcaaATCGACCTCAGGGTGTCGCAATTGATGATGGCGAAGGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACTAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGATGGC‑a                                                                        <  2:224775/140‑1 (MQ=255)                                                                                     gcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑a                                                                        >  1:224775/1‑140 (MQ=255)                                                                                          aCGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                          <  3:113361/149‑1 (MQ=255)                                                                                                  aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                                  >  1:36676/1‑149 (MQ=255)                                                                                                  aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                                  <  1:181160/149‑1 (MQ=255)                                                                                                  aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCCGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAAc                                                    <  2:285572/147‑1 (MQ=255)                                                                                                        tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACTACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                             <  6:266058/148‑1 (MQ=255)                                                                                                        tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                       <  2:32607/122‑1 (MQ=255)                                                                                                        tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                       >  1:32607/1‑122 (MQ=255)                                                                                                        tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                             <  6:253615/148‑1 (MQ=255)                                                                                                          gCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCgccgcc                                          >  5:30591/1‑149 (MQ=255)                                                                                                            aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                        >  8:257481/1‑149 (MQ=255)                                                                                                            aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                        >  6:107158/1‑149 (MQ=255)                                                                                                             aTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTg                                       >  8:164874/1‑149 (MQ=255)                                                                                                                gatgatGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                     <  3:194037/148‑1 (MQ=255)                                                                                                                 atgatgGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgccgc                                   >  5:87376/1‑149 (MQ=255)                                                                                                                         cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                            >  4:165932/1‑148 (MQ=255)                                                                                                                         cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                            >  1:245136/1‑148 (MQ=255)                                                                                                                              agaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCg                       >  4:160591/1‑148 (MQ=255)                                                                                                                               gaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                     >  8:295938/1‑149 (MQ=255)                                                                                                                                 atgTCTGGCGCAGCGAGGTATCCATCCCCGCTGGCCTGGACAAGCTCTGGGAACCCATGCAGGAATGCGTCAACCACGGCCTCAATTACGCGGGCAACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                     <  1:246487/146‑1 (MQ=255)                                                                                                                                 aGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCTGc                   <  8:37586/149‑1 (MQ=255)                                                                                                                                   gTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCt                 <  3:160591/149‑1 (MQ=255)                                                                                                                                    tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                     >  8:26407/1‑144 (MQ=255)                                                                                                                                    tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                     <  7:26407/144‑1 (MQ=255)                                                                                                                                      caggccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTg                >  4:239385/6‑147 (MQ=255)                                                                                                                                        gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGc               <  3:88136/146‑1 (MQ=255)                                                                                                                                        gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCa              <  8:225695/147‑1 (MQ=255)                                                                                                                                        gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg             >  1:71694/1‑148 (MQ=255)                                                                                                                                         gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                     >  7:37009/3‑123 (MQ=255)                                                                                                                                         gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                     <  8:37009/121‑1 (MQ=255)                                                                                                                                              cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGCGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg      <  4:101301/149‑1 (MQ=255)                                                                                                                                              cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg      <  7:164874/149‑1 (MQ=255)                                                                                                                                                 ggAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                          <  2:10209/94‑1 (MQ=255)                                                                                                                                                 ggAAGACATCCGCGCTGGCCTGCACAAACTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                          >  1:10209/1‑94 (MQ=255)                                                                                                                                                  gAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCaa  <  7:257481/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AGCCGCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAA  >  NC_002947/357123‑357416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑