| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,614,794 | T→C | 17.7% | F389L (TTC→CTC) | PP_4085 → | rhs family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,614,794 | 0 | T | C | 17.7% | 72.9 / 9.6 | 36 | F389L (TTC→CTC) | PP_4085 | rhs family protein |
| Reads supporting (aligned to +/- strand): ref base T (17/12); new base C (3/3); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
CTATGAAGTGGAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACG > NC_002947/4614652‑4614941 | cTATGAAGTGGAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGc < 8:9561/149‑1 (MQ=255) gCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCGTCCTCCAGCTGGAAGAAGGCCaag < 1:19294/148‑3 (MQ=255) ccGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCg > 7:37443/1‑149 (MQ=255) acaTCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCg > 2:47706/1‑146 (MQ=255) cAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAg < 6:247474/114‑1 (MQ=255) cAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAg > 5:247474/1‑114 (MQ=255) aaGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTg < 8:261045/116‑1 (MQ=255) aaGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTg > 7:261045/1‑116 (MQ=255) ggATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGtt > 6:22944/1‑119 (MQ=255) ggATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGtt < 5:22944/119‑1 (MQ=255) aCATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCTTGGACGACTTGGCCTTATTCCAGCTGGAGGACGGCCAGACCGTTTTAGAAg > 3:26735/1‑120 (MQ=255) aCATCGAAAACGACGGTAACGGACTGGAACTGGGCCGCCTAAGTGCCGGTGATGCCTTCATCAGTGCAGTGGACTGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAg < 4:26735/120‑1 (MQ=255) cATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCttt < 1:132433/137‑1 (MQ=255) cATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCttt > 2:132433/1‑137 (MQ=255) cAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGat > 8:78519/1‑115 (MQ=255) cAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGat < 7:78519/115‑1 (MQ=255) ggAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAAc > 2:210607/1‑149 (MQ=255) gAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGAGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTATTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAAACCGAAACg > 4:238370/1‑149 (MQ=255) tGGGCCGCCTCAGTGCCGGTGATGCCTTCATGAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCa > 1:91743/1‑149 (MQ=255) gccTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCt > 5:293363/1‑148 (MQ=255) ccGGTGATGCCTTCATCAGTGCCGTGGACGGCTGGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTCTCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGcgc < 6:159849/149‑3 (MQ=255) cGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCg > 2:191653/1‑149 (MQ=255) tGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGc > 2:270046/1‑148 (MQ=255) aTGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGAc < 1:191653/149‑1 (MQ=255) cTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGc > 4:273775/1‑148 (MQ=255) catcaGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAAt < 1:177293/148‑1 (MQ=255) aCGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGAc < 3:273775/149‑1 (MQ=255) ggCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACct > 8:92121/1‑149 (MQ=255) tctGGCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTc < 5:24794/140‑1 (MQ=255) tctGGCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTc > 6:24794/3‑142 (MQ=255) tctGGCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgc > 1:224613/3‑149 (MQ=255) cTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgc > 6:2071/1‑149 (MQ=255) ttGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgct < 1:47706/149‑1 (MQ=255) gCCTTCATCCAGCTGGACGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGt < 4:189957/148‑1 (MQ=255) gCCGTCCTCGAGCTGTAAGAAGGCCAGACCGTTGTCGTAGATATCTATGTAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAATCGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGcgt < 4:46862/137‑3 (MQ=255) gCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTAGGCGACCGCAATCTAAATTGCCt > 3:46862/1‑137 (MQ=255) cttcttCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTacg > 3:249166/1‑149 (MQ=255) ttcCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTacgacg > 3:182364/1‑148 (MQ=255) | CTATGAAGTGGAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACG > NC_002947/4614652‑4614941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |