Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	357,272	A→T	7.4%	D206V (GAC→GTC)	tdcG‑I →	L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	357,272	0	A	T	7.4%	196.7 / 4.7	68	D206V (GAC→GTC)	tdcG‑I	L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base A (36/26);  new base T (3/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCC  >  NC_002947/357127‑357419                                                                                                                                                  |                                                                                                                                                     gccAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACAt                                                                                                                                                   >  1:285572/1‑149 (MQ=255)       ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCgcgc                                                                                                                                             <  7:225127/149‑1 (MQ=255)       ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATACgcgc                                                                                                                                             >  6:150529/1‑149 (MQ=255)        gtgtGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCCCt                                                                                                                                            >  7:225695/1‑149 (MQ=255)              tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                          <  7:220386/145‑2 (MQ=255)              tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                          >  8:220386/1‑144 (MQ=255)                  tGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGcac                                                                                                                                   >  4:194037/1‑148 (MQ=255)                    cTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAg                                                                                                                                >  2:181160/1‑149 (MQ=255)                     tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                                     <  6:236233/143‑1 (MQ=255)                     tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                                     >  5:236233/1‑143 (MQ=255)                                       gTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAg                                                                                                              >  2:246487/1‑148 (MQ=255)                                              ttCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                      >  8:29611/1‑149 (MQ=255)                                              ttCAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                      >  1:288084/1‑149 (MQ=255)                                              ttCAACAGAGCCGCAGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAAAGAGAATGTCTGGCGAAGCGATGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                      <  5:150529/149‑1 (MQ=255)                                                cAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTc                                                                                                     <  2:288084/148‑1 (MQ=255)                                                 aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                       >  1:283213/1‑145 (MQ=255)                                                 aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                       <  2:283213/145‑1 (MQ=255)                                                  aCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaa                                                                                                   >  5:253615/1‑148 (MQ=255)                                                    aGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaacaa                                                                                                >  7:37586/1‑149 (MQ=255)                                                      cgccgcCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACg                                                                                              >  5:332638/1‑149 (MQ=255)                                                       gccgccGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACgg                                                                                             >  3:101301/1‑149 (MQ=255)                                                           ccgAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTc                                                                                         <  6:332638/149‑1 (MQ=255)                                                                   ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                                    >  8:253364/1‑130 (MQ=255)                                                                   ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                                    <  7:253364/130‑1 (MQ=255)                                                                   ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTAc                                                                                   >  4:113361/1‑147 (MQ=255)                                                                    tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAg                                                                                >  7:280070/1‑149 (MQ=255)                                                                             gcaagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTa                                                                      >  5:266058/1‑149 (MQ=255)                                                                              caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                       <  3:278649/132‑1 (MQ=255)                                                                              caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                       >  4:278649/1‑132 (MQ=255)                                                                                 gcaaATCGACCTCAGGGTGTCGCAATTGATGATGGCGAAGGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACTAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGATGGC‑a                                                                           <  2:224775/140‑1 (MQ=255)                                                                                 gcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑a                                                                           >  1:224775/1‑140 (MQ=255)                                                                                      aCGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                             <  3:113361/149‑1 (MQ=255)                                                                                              aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                                     <  1:181160/149‑1 (MQ=255)                                                                                              aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                                     >  1:36676/1‑149 (MQ=255)                                                                                              aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCCGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAAc                                                       <  2:285572/147‑1 (MQ=255)                                                                                                    tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACTACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                                <  6:266058/148‑1 (MQ=255)                                                                                                    tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                          >  1:32607/1‑122 (MQ=255)                                                                                                    tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                          <  2:32607/122‑1 (MQ=255)                                                                                                    tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                                <  6:253615/148‑1 (MQ=255)                                                                                                      gCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCgccgcc                                             >  5:30591/1‑149 (MQ=255)                                                                                                        aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                           >  8:257481/1‑149 (MQ=255)                                                                                                        aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                           >  6:107158/1‑149 (MQ=255)                                                                                                         aTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTg                                          >  8:164874/1‑149 (MQ=255)                                                                                                            gatgatGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                        <  3:194037/148‑1 (MQ=255)                                                                                                             atgatgGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgccgc                                      >  5:87376/1‑149 (MQ=255)                                                                                                                     cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                               >  4:165932/1‑148 (MQ=255)                                                                                                                     cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                               >  1:245136/1‑148 (MQ=255)                                                                                                                          agaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCg                          >  4:160591/1‑148 (MQ=255)                                                                                                                           gaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                        >  8:295938/1‑149 (MQ=255)                                                                                                                             atgTCTGGCGCAGCGAGGTATCCATCCCCGCTGGCCTGGACAAGCTCTGGGAACCCATGCAGGAATGCGTCAACCACGGCCTCAATTACGCGGGCAACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                        <  1:246487/146‑1 (MQ=255)                                                                                                                             aGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCTGc                      <  8:37586/149‑1 (MQ=255)                                                                                                                               gTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCt                    <  3:160591/149‑1 (MQ=255)                                                                                                                                tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                        <  7:26407/144‑1 (MQ=255)                                                                                                                                tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                        >  8:26407/1‑144 (MQ=255)                                                                                                                                  caggccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTg                   >  4:239385/6‑147 (MQ=255)                                                                                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGc                  <  3:88136/146‑1 (MQ=255)                                                                                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCa                 <  8:225695/147‑1 (MQ=255)                                                                                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg                >  1:71694/1‑148 (MQ=255)                                                                                                                                     gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                        >  7:37009/3‑123 (MQ=255)                                                                                                                                     gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                        <  8:37009/121‑1 (MQ=255)                                                                                                                                          cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGCGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg         <  4:101301/149‑1 (MQ=255)                                                                                                                                          cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg         <  7:164874/149‑1 (MQ=255)                                                                                                                                             ggAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                             <  2:10209/94‑1 (MQ=255)                                                                                                                                             ggAAGACATCCGCGCTGGCCTGCACAAACTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                             >  1:10209/1‑94 (MQ=255)                                                                                                                                              gAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCaa     <  7:257481/149‑1 (MQ=255)                                                                                                                                                 gACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATc          <  2:35291/141‑1 (MQ=255)                                                                                                                                                 gACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCgagaacg   >  1:35291/1‑142 (MQ=255)                                                                                                                                                 gACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGcc  >  1:155994/1‑149 (MQ=255)                                                                                                                                                  aCATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGcc  >  5:65373/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GCCAGCGGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCC  >  NC_002947/357127‑357419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑