Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,649,982 T→G 15.0% H311P (CAC→CCC)  PP_4113 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,649,9820TG15.0% 119.0 / 9.0 46H311P (CAC→CCC) PP_4113hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base G (3/4);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCG  >  NC_002947/4649834‑4650129
                                                                                                                                                    |                                                                                                                                                   
gTTGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCtgt                                                                                                                                                     <  2:95087/149‑1 (MQ=255)
      aTCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCTCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGc                                                                                                                                               >  4:6851/1‑149 (MQ=255)
       tCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGCCCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGcc                                                                                                                                              >  7:296725/1‑149 (MQ=255)
                 cGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCGGGGCTGGGCAAGCACAGCCCTGGc                                                                                                                                    <  2:112643/149‑1 (MQ=255)
                      cGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGGGCAAGCACAGccctg                                                                                                                                      <  6:194835/142‑3 (MQ=255)
                      cGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGGGCAAGCACAGccctg                                                                                                                                      >  5:194835/1‑140 (MQ=255)
                       ggTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGc                                                                                                                                                   <  8:5372/128‑1 (MQ=255)
                       ggTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGc                                                                                                                                                   >  7:5372/1‑128 (MQ=255)
                         tGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGcc                                                                                                                            <  7:9153/149‑1 (MQ=255)
                                        cAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGc                                                                                                             >  7:27169/1‑149 (MQ=255)
                                         aGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCg                                                                                                            <  3:239796/149‑1 (MQ=255)
                                              aGCACCCGCACGCCATCATCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAAt                                                                                                       <  2:314241/149‑1 (MQ=255)
                                                     gCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGc                                                                                                 >  8:227065/1‑148 (MQ=255)
                                                       aCGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTg                                                                                                                                                >  3:86509/1‑99 (MQ=255)
                                                       aCGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTg                                                                                                                                                <  4:86509/99‑1 (MQ=255)
                                                               ttCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGc                                                                                      >  5:82089/1‑149 (MQ=255)
                                                                   aCCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTg                                                                                  >  6:315960/1‑149 (MQ=255)
                                                                        gTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCAcc                                                                                                                      <  6:187485/108‑1 (MQ=255)
                                                                        gTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCAcc                                                                                                                      >  5:187485/1‑108 (MQ=255)
                                                                         ttCACCGAGGCTTCGATGTTGGCGGTGATTCCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGATTGCCTGCGACATGGCTGCCGTGGCTTGc                                                                            <  8:296725/149‑1 (MQ=255)
                                                                            aCCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCg                                                                                    >  4:204508/1‑138 (MQ=255)
                                                                            aCCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCg                                                                                    <  3:204508/138‑1 (MQ=255)
                                                                                aGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCgg                                                                     <  3:230499/149‑1 (MQ=255)
                                                                                    aGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTAc                                                                 >  2:144798/1‑149 (MQ=255)
                                                                                        aTGTTCGCGGTGATACCGGCATCCGAGATGCGCTCTACCATCACCAAGAAGCCAGGGCAGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTc                                                             >  6:314329/1‑149 (MQ=255)
                                                                                           ttGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTcgc                                                           <  8:27169/148‑1 (MQ=255)
                                                                                                ggTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGt                                                     <  3:6851/149‑1 (MQ=255)
                                                                                                      aCCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAgg                                                >  1:23831/1‑148 (MQ=255)
                                                                                                             tCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTg                                        >  4:71763/1‑149 (MQ=255)
                                                                                                              ccGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGc                                                                       >  5:79019/1‑117 (MQ=255)
                                                                                                              ccGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGc                                                                       <  6:79019/117‑1 (MQ=255)
                                                                                                                     gcgcTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGt                                 <  5:315960/148‑1 (MQ=255)
                                                                                                                        ctctACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCg                              >  2:28204/1‑148 (MQ=255)
                                                                                                                           tACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                            <  2:321499/147‑1 (MQ=255)
                                                                                                                             ccATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGccc                        >  5:326967/1‑149 (MQ=255)
                                                                                                                                    cTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTaga                                                  <  6:247355/116‑1 (MQ=255)
                                                                                                                                    cTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTaga                                                  >  5:247355/1‑116 (MQ=255)
                                                                                                                                    cTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGa                  >  6:144129/1‑148 (MQ=255)
                                                                                                                                      cGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATaa               >  7:121536/1‑149 (MQ=255)
                                                                                                                                          gCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGc           >  2:62982/1‑149 (MQ=255)
                                                                                                                                           ccAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt          <  1:62982/149‑1 (MQ=255)
                                                                                                                                            cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGc                             <  3:292556/127‑1 (MQ=255)
                                                                                                                                            cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCAGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGc                             >  4:292556/3‑129 (MQ=255)
                                                                                                                                            cggggCTGGGCAAGCACAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt          >  4:276771/3‑148 (MQ=255)
                                                                                                                                            cggggCTGGGCAAGCACAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTg         <  3:276771/147‑1 (MQ=255)
                                                                                                                                            cAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                            <  2:64226/130‑1 (MQ=255)
                                                                                                                                            cAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                            >  1:64226/1‑130 (MQ=255)
                                                                                                                                                  tgtgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAcccc   <  8:121536/149‑1 (MQ=255)
                                                                                                                                                  tgtgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAcccc   >  1:239215/1‑149 (MQ=255)
                                                                                                                                                    tgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCg  <  6:326967/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCG  >  NC_002947/4649834‑4650129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑