Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,649,986 T→A 13.7% K310* (AAG→TAG) ‡ PP_4113 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,649,9860TA13.7% 127.1 / 10.3 51K310* (AAG→TAG) ‡PP_4113hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/19);  new base A (3/4);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCGGGG  >  NC_002947/4649840‑4650132
                                                                                                                                                  |                                                                                                                                                  
aTCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCTCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGc                                                                                                                                                  >  4:6851/1‑149 (MQ=255)
 tCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGCCCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGcc                                                                                                                                                 >  7:296725/1‑149 (MQ=255)
           cGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCGGGGCTGGGCAAGCACAGCCCTGGc                                                                                                                                       <  2:112643/149‑1 (MQ=255)
                cGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGGGCAAGCACAGccctg                                                                                                                                         >  5:194835/1‑140 (MQ=255)
                cGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGGGCAAGCACAGccctg                                                                                                                                         <  6:194835/142‑3 (MQ=255)
                   tGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGcc                                                                                                                               <  7:9153/149‑1 (MQ=255)
                                  cAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGc                                                                                                                >  7:27169/1‑149 (MQ=255)
                                   aGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCg                                                                                                               <  3:239796/149‑1 (MQ=255)
                                        aGCACCCGCACGCCATCATCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAAt                                                                                                          <  2:314241/149‑1 (MQ=255)
                                               gCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGc                                                                                                    >  8:227065/1‑148 (MQ=255)
                                                 aCGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTg                                                                                                                                                   >  3:86509/1‑99 (MQ=255)
                                                 aCGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTg                                                                                                                                                   <  4:86509/99‑1 (MQ=255)
                                                         ttCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGc                                                                                         >  5:82089/1‑149 (MQ=255)
                                                             aCCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTg                                                                                     >  6:315960/1‑149 (MQ=255)
                                                                  gTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCAcc                                                                                                                         >  5:187485/1‑108 (MQ=255)
                                                                  gTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCAcc                                                                                                                         <  6:187485/108‑1 (MQ=255)
                                                                   ttCACCGAGGCTTCGATGTTGGCGGTGATTCCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGATTGCCTGCGACATGGCTGCCGTGGCTTGc                                                                               <  8:296725/149‑1 (MQ=255)
                                                                      aCCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCg                                                                                       >  4:204508/1‑138 (MQ=255)
                                                                      aCCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCg                                                                                       <  3:204508/138‑1 (MQ=255)
                                                                          aGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCgg                                                                        <  3:230499/149‑1 (MQ=255)
                                                                              aGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTAc                                                                    >  2:144798/1‑149 (MQ=255)
                                                                                  aTGTTCGCGGTGATACCGGCATCCGAGATGCGCTCTACCATCACCAAGAAGCCAGGGCAGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTc                                                                >  6:314329/1‑149 (MQ=255)
                                                                                     ttGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTcgc                                                              <  8:27169/148‑1 (MQ=255)
                                                                                          ggTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGt                                                        <  3:6851/149‑1 (MQ=255)
                                                                                                aCCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAgg                                                   >  1:23831/1‑148 (MQ=255)
                                                                                                       tCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTg                                           >  4:71763/1‑149 (MQ=255)
                                                                                                        ccGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGc                                                                          >  5:79019/1‑117 (MQ=255)
                                                                                                        ccGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGc                                                                          <  6:79019/117‑1 (MQ=255)
                                                                                                               gcgcTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGt                                    <  5:315960/148‑1 (MQ=255)
                                                                                                                  ctctACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCg                                 >  2:28204/1‑148 (MQ=255)
                                                                                                                     tACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                               <  2:321499/147‑1 (MQ=255)
                                                                                                                       ccATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGccc                           >  5:326967/1‑149 (MQ=255)
                                                                                                                              cTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTaga                                                     >  5:247355/1‑116 (MQ=255)
                                                                                                                              cTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTaga                                                     <  6:247355/116‑1 (MQ=255)
                                                                                                                              cTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGa                     >  6:144129/1‑148 (MQ=255)
                                                                                                                                cGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATaa                  >  7:121536/1‑149 (MQ=255)
                                                                                                                                    gCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGc              >  2:62982/1‑149 (MQ=255)
                                                                                                                                     ccAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt             <  1:62982/149‑1 (MQ=255)
                                                                                                                                      cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGc                                <  3:292556/127‑1 (MQ=255)
                                                                                                                                      cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCAGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGc                                >  4:292556/3‑129 (MQ=255)
                                                                                                                                      cggggCTGGGCAAGCACAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt             >  4:276771/3‑148 (MQ=255)
                                                                                                                                      cggggCTGGGCAAGCACAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTg            <  3:276771/147‑1 (MQ=255)
                                                                                                                                      cAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                               <  2:64226/130‑1 (MQ=255)
                                                                                                                                      cAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                               >  1:64226/1‑130 (MQ=255)
                                                                                                                                            tgtgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAcccc      <  8:121536/149‑1 (MQ=255)
                                                                                                                                            tgtgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAcccc      >  1:239215/1‑149 (MQ=255)
                                                                                                                                              tgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCg     <  6:326967/148‑1 (MQ=255)
                                                                                                                                               gCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCggg   >  7:816/1‑149 (MQ=255)
                                                                                                                                                cTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAccc       <  2:302886/144‑1 (MQ=255)
                                                                                                                                                cTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAccc       >  1:302886/1‑144 (MQ=255)
                                                                                                                                                cTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCgggg  >  4:169058/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCCGGGG  >  NC_002947/4649840‑4650132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑