| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,654,891 | T→C | 7.1% | intergenic (+43/‑215) | aceA → / → PP_4118 | isocitrate lyase/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,654,891 | 0 | T | C | 7.1% | 163.6 / 2.5 | 56 | intergenic (+43/‑215) | aceA/PP_4118 | isocitrate lyase/membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (24/28); new base C (2/2); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTGGGCGCAGGCC > NC_002947/4654743‑4655039 | ggAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCt > 7:223910/1‑149 (MQ=255) ggCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGc < 1:207788/148‑1 (MQ=255) cACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTAAAAACAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCt < 6:110673/149‑1 (MQ=37) cACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCtt > 2:277751/1‑149 (MQ=255) gCTACTTCGACGACATGACCACCGTGATCCAGGGGGGCGGTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGtt < 2:103664/148‑1 (MQ=255) gCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCGCGTGGCTTGTGAAAGCTCCGGGCCTTTGttt < 4:40916/149‑1 (MQ=255) gCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGttt > 5:124253/1‑149 (MQ=255) gCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGttt < 6:52969/149‑1 (MQ=255) tCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTAccc > 4:221042/1‑149 (MQ=255) cgacgaCATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTAccc > 1:16156/1‑148 (MQ=255) gacgacATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga < 3:52698/149‑1 (MQ=255) gacgacATGACCACCGT‑ATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTAccc > 7:116980/1‑146 (MQ=255) acgacATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga < 8:223910/148‑1 (MQ=255) cATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAg < 4:118932/149‑1 (MQ=255) tGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAggg < 1:277751/149‑1 (MQ=255) ccGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgggccgggc < 3:221042/149‑1 (MQ=255) tGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCctg < 7:261060/149‑1 (MQ=255) aTCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCTGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCctgct > 5:35823/1‑149 (MQ=255) tCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCctgctg < 6:124253/149‑1 (MQ=255) gcTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTa < 8:116980/149‑1 (MQ=255) gcTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTa < 2:16156/149‑1 (MQ=255) aCTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCAc > 2:19554/1‑149 (MQ=255) tGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCg > 8:81360/1‑149 (MQ=255) tGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTcc > 4:13033/1‑149 (MQ=255) gACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTAcc < 7:81360/148‑1 (MQ=255) cGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTAt > 7:25663/1‑149 (MQ=255) gaagaaGAGCAGTTCCACTGATAGGGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTAtt < 5:165211/149‑1 (MQ=255) aagaagAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTc > 4:240295/1‑149 (MQ=255) gCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACACGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGt > 5:198006/1‑148 (MQ=255) gTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTccgc < 4:88619/126‑1 (MQ=255) gTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTccgc > 3:88619/1‑126 (MQ=255) cACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTTGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGc < 8:113847/149‑1 (MQ=255) cACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCg > 6:180494/1‑148 (MQ=255) cACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGc < 4:108520/149‑1 (MQ=255) tGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCAcc > 7:127658/1‑149 (MQ=255) aTAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTa < 3:13033/149‑1 (MQ=255) tAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCTGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTac < 6:35823/149‑1 (MQ=255) tAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTac < 8:25663/149‑1 (MQ=255) aGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTaca > 7:29227/1‑149 (MQ=255) gTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTacac < 3:239741/149‑1 (MQ=255) gatGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGc < 6:33878/135‑1 (MQ=255) ccGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCggg > 6:175129/1‑149 (MQ=255) aCAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGgaga > 7:114398/1‑148 (MQ=255) aaaGGCCCGGGGCTTGTGAAAGCTCCGAGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGaca > 8:25392/1‑148 (MQ=255) aaaGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGaca > 8:78269/1‑148 (MQ=21) aGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGacaca > 2:289502/1‑148 (MQ=255) gCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACAt > 5:41125/1‑149 (MQ=255) gCCCGGGGCTTGTGAAAGCTCCGAGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACAt < 7:25392/149‑1 (MQ=255) ccGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCg > 7:211450/1‑149 (MQ=255) ccGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCg > 3:268864/1‑149 (MQ=255) cGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCgg < 6:198006/149‑1 (MQ=255) ggCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGa < 8:114398/149‑1 (MQ=255) ctttcacaaGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGAtt > 3:81322/8‑149 (MQ=255) tcacaaGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTgg < 7:78269/144‑1 (MQ=255) gtgAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAAcc < 3:152058/106‑1 (MQ=255) gtgAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAAcc > 4:152058/1‑106 (MQ=255) gtgAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTgg < 3:240295/148‑1 (MQ=255) tCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTGGGCGCAGGcc > 1:170992/1‑149 (MQ=255) | GGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGT‑TGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTGGGCGCAGGCC > NC_002947/4654743‑4655039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |