Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,684,796 G→A 11.4% V479M (GTG→ATG)  PP_4146 → peptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,684,7960GA11.4% 87.3 / 3.1 35V479M (GTG→ATG) PP_4146peptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (14/17);  new base A (2/2);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

GAAGCTGCACGGCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACTTCCATTCCAGCCAGGCCGCGACAAAGGG  >  NC_002947/4684651‑4684929
                                                                                                                                                  |                                                                                                                                     
gAAGCTGCACGGCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTg                                                                                                                                     <  2:109042/148‑1 (MQ=255)
   gCTGCACGGCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGAcc                                                                                                                                 <  5:127608/149‑1 (MQ=255)
      gCACGGCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTg                                                                                                                              >  8:199655/1‑149 (MQ=255)
        aCGGCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGcc                                                                                                                            <  5:127717/149‑1 (MQ=255)
           gCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAg                                                                                                                         >  3:295682/1‑149 (MQ=255)
              aGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTa                                                                                                                       >  6:209765/1‑148 (MQ=255)
                         gACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCt                                                                                                           <  5:209765/149‑1 (MQ=255)
                                 gggCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGt                                                                                                   <  8:15871/149‑1 (MQ=255)
                                   gCCAGCAATTGCGCATGGAACTGCTGCTGGTAAAGCCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTac                                                                                                                      >  1:206251/1‑129 (MQ=255)
                                   gCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTac                                                                                                                      <  2:206251/128‑1 (MQ=255)
                                    ccAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGATCGTGCCCa                                                                                                                          >  4:155088/1‑123 (MQ=255)
                                    ccAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGATCGTGCCCa                                                                                                                          <  3:155088/123‑1 (MQ=255)
                                       gCAATTGTGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGAtt                                                                                             <  4:192708/149‑1 (MQ=255)
                                              cgcATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACAt                                                                                      <  7:199655/149‑1 (MQ=255)
                                                cATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGa                                                                                    <  7:69119/149‑1 (MQ=255)
                                                cATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCACACTACAAACAACGTCAGGACCAGTTCGATTTCGACATg                                                                                     >  4:221803/1‑148 (MQ=255)
                                                     aaCTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCtg                                                                               <  7:21752/149‑1 (MQ=255)
                                                       gtgctgctgGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCtgat                                                                             <  1:165474/148‑1 (MQ=255)
                                                             ctgGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCt                                                                       >  2:117799/1‑149 (MQ=255)
                                                              tgGTAAA‑CCCCAACCTAGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTATGGCCCAGTTAGATTTCGACATGATTCTGATGACACt                                                                       >  6:92654/1‑148 (MQ=255)
                                                                gTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGaa                                                                    >  4:310457/1‑149 (MQ=255)
                                                                    a‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTTGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAg                                                                <  3:221803/149‑1 (MQ=255)
                                                                                  aCGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCg                                                   <  4:295682/149‑1 (MQ=255)
                                                                                   cGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCATGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCgg                                                  >  4:294175/1‑149 (MQ=255)
                                                                                   cGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCATGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCgg                                                  >  4:298812/1‑149 (MQ=255)
                                                                                      aTCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCt                                               <  5:100317/149‑1 (MQ=255)
                                                                                               ccTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCATGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATg                                      <  3:298812/149‑1 (MQ=255)
                                                                                               ccTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCATGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATg                                      <  3:294175/149‑1 (MQ=255)
                                                                                                              cTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACttccattc                       >  1:318416/1‑149 (MQ=255)
                                                                                                               tGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACttccattc                       >  4:256023/1‑148 (MQ=255)
                                                                                                                   cAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCt                                   >  5:17068/1‑132 (MQ=255)
                                                                                                                   cAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCt                                   <  6:17068/132‑1 (MQ=255)
                                                                                                                     gcatcggcatcgATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACTTCCATTccagccag                >  8:252252/1‑149 (MQ=255)
                                                                                                                       atcggcatcgATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACTTCCATTCCAGCCAGGc              <  3:310457/149‑1 (MQ=255)
                                                                                                                                     gcgcTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACTTCCATTCCAGCCAGGCCGCGACAAAggg  >  3:312276/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                     
GAAGCTGCACGGCCAGCGCCTGGTCGACAGCAAGGGCCAGCAATTGCGCATGGAACTGCTGCTGGTAAA‑CCCCAACCTTGAACGCATCCTGCAACCTTATGTCGAAAACCTGTCCAGCATCGGCATCGATGCGCGCTTGCGCACCGTGGACCGTGCCCAGTACAAACAACGTCTGGACCAGTTCGATTTCGACATGATTCTGATGACCCTGAACCAGACCCTCAGCCCCGGCCTCGAACAATGGCTGTACTTCCATTCCAGCCAGGCCGCGACAAAGGG  >  NC_002947/4684651‑4684929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑