Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	357,278	G→T	7.4%	R208L (CGC→CTC)	tdcG‑I →	L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	357,278	0	G	T	7.4%	209.9 / 5.5	68	R208L (CGC→CTC)	tdcG‑I	L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base G (36/26);  new base T (3/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

GGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGT  >  NC_002947/357133‑357425                                                                                                                                                  |                                                                                                                                                     ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCgcgc                                                                                                                                                   <  7:225127/149‑1 (MQ=255) ggTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATACgcgc                                                                                                                                                   >  6:150529/1‑149 (MQ=255)  gtgtGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCCCt                                                                                                                                                  >  7:225695/1‑149 (MQ=255)        tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                                <  7:220386/145‑2 (MQ=255)        tGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGCGGATGTCTTCCTCGCTgc                                                                                                                                                >  8:220386/1‑144 (MQ=255)            tGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGcac                                                                                                                                         >  4:194037/1‑148 (MQ=255)              cTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAg                                                                                                                                      >  2:181160/1‑149 (MQ=255)               tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                                           >  5:236233/1‑143 (MQ=255)               tGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGc                                                                                                                                           <  6:236233/143‑1 (MQ=255)                                 gTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAg                                                                                                                    >  2:246487/1‑148 (MQ=255)                                        ttCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                            >  8:29611/1‑149 (MQ=255)                                        ttCAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                            >  1:288084/1‑149 (MQ=255)                                        ttCAACAGAGCCGCAGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAAAGAGAATGTCTGGCGAAGCGATGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGt                                                                                                            <  5:150529/149‑1 (MQ=255)                                          cAACAGCGCCGCCGAACTGCTCCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTc                                                                                                           <  2:288084/148‑1 (MQ=255)                                           aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                             >  1:283213/1‑145 (MQ=255)                                           aaCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCg                                                                                                             <  2:283213/145‑1 (MQ=255)                                            aCAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaa                                                                                                         >  5:253615/1‑148 (MQ=255)                                              aGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTcaacaa                                                                                                      >  7:37586/1‑149 (MQ=255)                                                cgccgcCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACg                                                                                                    >  5:332638/1‑149 (MQ=255)                                                 gccgccGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACgg                                                                                                   >  3:101301/1‑149 (MQ=255)                                                     ccgAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTc                                                                                               <  6:332638/149‑1 (MQ=255)                                                             ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                                          <  7:253364/130‑1 (MQ=255)                                                             ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTca                                                                                                          >  8:253364/1‑130 (MQ=255)                                                             ctgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTAc                                                                                         >  4:113361/1‑147 (MQ=255)                                                              tgcGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAg                                                                                      >  7:280070/1‑149 (MQ=255)                                                                       gcaagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTa                                                                            >  5:266058/1‑149 (MQ=255)                                                                        caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                             <  3:278649/132‑1 (MQ=255)                                                                        caagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCaa                                                                                             >  4:278649/1‑132 (MQ=255)                                                                           gcaaATCGACCTCAGGGTGTCGCAATTGATGATGGCGAAGGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACTAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGATGGC‑a                                                                                 <  2:224775/140‑1 (MQ=255)                                                                           gcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑a                                                                                 >  1:224775/1‑140 (MQ=255)                                                                                aCGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                                   <  3:113361/149‑1 (MQ=255)                                                                                        aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                                           <  1:181160/149‑1 (MQ=255)                                                                                        aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGt                                                           >  1:36676/1‑149 (MQ=255)                                                                                        aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCCGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAAc                                                             <  2:285572/147‑1 (MQ=255)                                                                                              tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACTACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                                      <  6:266058/148‑1 (MQ=255)                                                                                              tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                                <  2:32607/122‑1 (MQ=255)                                                                                              tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑Ac                                                                                >  1:32607/1‑122 (MQ=255)                                                                                              tCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGcgcc                                                      <  6:253615/148‑1 (MQ=255)                                                                                                gCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCgccgcc                                                   >  5:30591/1‑149 (MQ=255)                                                                                                  aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                                 >  8:257481/1‑149 (MQ=255)                                                                                                  aaTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGt                                                 >  6:107158/1‑149 (MQ=255)                                                                                                   aTTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTg                                                >  8:164874/1‑149 (MQ=255)                                                                                                      gatgatGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                              <  3:194037/148‑1 (MQ=255)                                                                                                       atgatgGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgccgc                                            >  5:87376/1‑149 (MQ=255)                                                                                                               cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                                     >  1:245136/1‑148 (MQ=255)                                                                                                               cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                                     >  4:165932/1‑148 (MQ=255)                                                                                                                    agaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCg                                >  4:160591/1‑148 (MQ=255)                                                                                                                     gaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                              >  8:295938/1‑149 (MQ=255)                                                                                                                       atgTCTGGCGCAGCGAGGTATCCATCCCCGCTGGCCTGGACAAGCTCTGGGAACCCATGCAGGAATGCGTCAACCACGGCCTCAATTACGCGGGCAACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                              <  1:246487/146‑1 (MQ=255)                                                                                                                       aGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCTGc                            <  8:37586/149‑1 (MQ=255)                                                                                                                         gTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCt                          <  3:160591/149‑1 (MQ=255)                                                                                                                          tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                              >  8:26407/1‑144 (MQ=255)                                                                                                                          tCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                              <  7:26407/144‑1 (MQ=255)                                                                                                                            caggccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTg                         >  4:239385/6‑147 (MQ=255)                                                                                                                              gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGc                        <  3:88136/146‑1 (MQ=255)                                                                                                                              gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCa                       <  8:225695/147‑1 (MQ=255)                                                                                                                              gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg                      >  1:71694/1‑148 (MQ=255)                                                                                                                               gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                              <  8:37009/121‑1 (MQ=255)                                                                                                                               gccAGCGCGGATGTCTTCCTCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgcc                                              >  7:37009/3‑123 (MQ=255)                                                                                                                                    cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGCGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg               <  4:101301/149‑1 (MQ=255)                                                                                                                                    cGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCg               <  7:164874/149‑1 (MQ=255)                                                                                                                                       ggAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                                   <  2:10209/94‑1 (MQ=255)                                                                                                                                       ggAAGACATCCGCGCTGGCCTGCACAAACTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCggg                                                                   >  1:10209/1‑94 (MQ=255)                                                                                                                                        gAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCaa           <  7:257481/149‑1 (MQ=255)                                                                                                                                           gACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATc                <  2:35291/141‑1 (MQ=255)                                                                                                                                           gACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCgagaacg         >  1:35291/1‑142 (MQ=255)                                                                                                                                           gACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGcc        >  1:155994/1‑149 (MQ=255)                                                                                                                                            aCATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGcc        >  5:65373/1‑148 (MQ=255)                                                                                                                                               tCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAAc    <  7:29611/149‑1 (MQ=255)                                                                                                                                                ccGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTACAGGAAATCGGCAAGCCCAACg   >  3:235561/1‑149 (MQ=255)                                                                                                                                                 cgcgCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGt  >  7:128859/1‑149 (MQ=255)                                                                                                                                                  gcgcTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgccg                                             >  2:110211/1‑105 (MQ=255)                                                                                                                                                  gcgcTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTgccg                                             <  1:110211/105‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GGTGTGCTGGATGCTGACCAGACAGTGCTGCCGTACGACTTCAACAGCGCCGCCGAACTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGC‑ACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGT  >  NC_002947/357133‑357425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑