| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,756,432 | A→C | 10.9% | E558A (GAG→GCG) ‡ | pvdT → | pyoverdine ABC transporter ATP‑binding protein/permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,756,432 | 0 | A | C | 10.9% | 151.6 / 10.6 | 55 | E558A (GAG→GCG) ‡ | pvdT | pyoverdine ABC transporter ATP‑binding protein/permease |
| Reads supporting (aligned to +/- strand): ref base A (27/22); new base C (2/4); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
TTTCGAGCTGACCAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACC > NC_002947/4756286‑4756580 | tttCGAGCTGACCAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAgc > 4:115271/1‑149 (MQ=255)tttCGAGCTGACCAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAgc < 1:29884/149‑1 (MQ=255) gAGCTGACCAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCAc < 1:283061/149‑1 (MQ=255) aCCAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCTCGCGCAcggtc < 3:222202/147‑5 (MQ=255) ccAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCTCGCGCAcggtcatg < 6:138724/149‑8 (MQ=255) cGATGCCGCCCTGATCCAGGACGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGTGTGATGTACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCg < 2:321893/149‑1 (MQ=255) aTGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATc > 1:289510/1‑146 (MQ=255) gccCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGCCGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAACTCGGTATCCg > 4:213425/1‑149 (MQ=255) gATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATgg > 4:112323/1‑149 (MQ=255) ccAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCAc < 5:66087/123‑1 (MQ=255) ccAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCAc > 6:66087/1‑123 (MQ=255) aGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCAcc > 1:304970/1‑149 (MQ=255) ccGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTCTCCGCATGGCCACCGgc > 4:258972/1‑149 (MQ=255) gcaGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAgcgc > 1:291855/1‑148 (MQ=255) gcaGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGcgcg < 2:291855/149‑1 (MQ=255) caGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGa < 1:16064/149‑1 (MQ=255) cAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGTAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACAtcc < 1:66244/149‑1 (MQ=255) cAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACAtcc > 5:147345/1‑149 (MQ=255) ccTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACAtcctcc > 7:180662/1‑149 (MQ=255) gCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCt > 7:35886/1‑147 (MQ=255) cTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCACCGGCAGTTCCTGAg > 4:84283/1‑149 (MQ=255) ggggCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGc < 8:35886/147‑1 (MQ=255) ggggCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGa < 6:103393/149‑1 (MQ=255) gggCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAg < 8:55865/149‑1 (MQ=255) ttGGCGCCATCTGGCTGCTGGGCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCACCGGCAGTTCCTGAGCGAGGCGATc < 3:84283/149‑1 (MQ=255) gccgccATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCa < 8:71628/148‑1 (MQ=255) gctgctGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGAt < 8:180662/147‑1 (MQ=255) gctgctGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATg < 3:115271/148‑1 (MQ=255) tCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTggcggc > 4:152524/1‑149 (MQ=255) cggTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGa > 7:51525/1‑149 (MQ=255) tATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCg < 8:51525/149‑1 (MQ=255) tCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCgg > 3:288240/1‑148 (MQ=255) cGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCa > 8:30250/1‑149 (MQ=255) gacgaACATCATGCGCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGGTGGCGGCCTGACCGGCATCgcc < 3:213425/145‑1 (MQ=255) atgaACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccct < 6:147345/149‑1 (MQ=255) atgaACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccct < 2:304970/149‑1 (MQ=255) atgaACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccct > 3:279839/1‑149 (MQ=255) atgaACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGCCGCCCGCCAGCGCGACATCCTCCGGCACTTCCTGAGCGAGGCGATCATGCTGTCGATCGTTGGCGGCCTGACCGGCATCgccct > 5:269779/1‑149 (MQ=255) aCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCt > 3:242178/1‑130 (MQ=255) aCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCt < 4:242178/130‑1 (MQ=255) aCATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggcc > 3:193333/1‑149 (MQ=255) catcatGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggccc < 5:161820/149‑1 (MQ=255) catcatGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggccc > 6:223048/1‑149 (MQ=255) atcatGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCgccctggccct > 1:189710/1‑149 (MQ=255) cTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTggtggt > 4:280013/1‑149 (MQ=255) aTGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCgg > 7:177840/1‑149 (MQ=255) aCCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGgcgc > 7:118096/1‑149 (MQ=255) aCCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGgcgc > 3:32092/1‑149 (MQ=255) gggTGCGCTCGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATg > 2:64519/3‑104 (MQ=255) gggTGCGCTCGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATg < 1:64519/102‑1 (MQ=255) gggTGCGCTCGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGgcg < 2:60585/145‑1 (MQ=255) gggTGCGCTCGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGgcg > 1:60585/3‑147 (MQ=255) cGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCa < 4:32092/149‑1 (MQ=255) gTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAg > 3:29563/1‑149 (MQ=255) tGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGc < 4:193333/149‑1 (MQ=255) aGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTAcc < 3:112323/149‑1 (MQ=255) aGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTAcc < 3:280013/149‑1 (MQ=255) | TTTCGAGCTGACCAACGATGCCGCCCTGATCCAGGCCGAGGCGCGCACGCAGAACAGCCTGTCGTTGATGCTCGGGGCCATTGCCGCCATCTCGCTGCTGGTCGGCGGTATCGGAGTGATGAACATCATGCTCATGACCGTGCGCGAGCGCACCCGTGAAATCGGTATCCGCATGGCCACCGGCGCCCGCCAGCGCGACATCCTCCGGCAGTTCCTGAGCGAGGCGATCATGCTGTCGATGGTTGGCGGCCTGACCGGCATCGCCCTGGCCCTGGTGGTCGGCGCCAGCCTTACC > NC_002947/4756286‑4756580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |