Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,758,141	G→C	9.2%	intergenic (+1/+20)	ompQ → / ← pvdP	outer membrane pyoverdine efflux protein/pyoverdine biosynthesis‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,758,141	0	G	C	9.2%	185.8 / 7.8	63	intergenic (+1/+20)	ompQ/pvdP	outer membrane pyoverdine efflux protein/pyoverdine biosynthesis‑like protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (3/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGAT  >  NC_002947/4757996‑4758288                                                                                                                                                  |                                                                                                                                                    cTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAggg                                                                                                                                                    >  6:24504/1‑147 (MQ=255) cTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAggg                                                                                                                                                    <  5:24504/147‑1 (MQ=255)        aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAgg                                                                                                                                             <  8:164767/147‑1 (MQ=255)        aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGc                                                                                                                                            >  8:34538/1‑148 (MQ=255)        aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGATGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGc                                                                                                                                            >  5:217487/1‑148 (MQ=255)        aCCGGGCGCGACACCCAGCGCAGCCTGTACCAGGCCCACGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAgg                                                                                                                                             <  8:52173/147‑1 (MQ=255)          cGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGCTAt                                                                                                                                         <  8:105644/149‑1 (MQ=255)          cGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                                                         >  4:226037/1‑149 (MQ=255)              cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTg                                                                                                                                      <  5:332560/148‑1 (MQ=255)                   aacCCAGCGCAGCCTGTACCAGGCGCAGGACCAGCAGGCCCGCCTGATGCTAAGCCAACTGCAGGCCAGCGTGGCCC‑TTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTc                                                                                                                                <  4:19925/147‑1 (MQ=255)                    acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCa                                                                                                                               >  4:261743/1‑149 (MQ=255)                    acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCa                                                                                                                               >  8:144788/1‑149 (MQ=255)                     cccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc                                                                                                                              <  5:267025/149‑1 (MQ=255)                          cgcAGGCTGAACCAGGCCCAGGACCAGCAGGCCCGCCTGAAGGTATCCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCATGCGTATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCg                                                                                                                         <  4:186008/149‑1 (MQ=255)                           gcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGc                                                                                                                        <  7:144788/149‑1 (MQ=255)                                cTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCt                                                                                                                   <  4:190365/149‑1 (MQ=255)                                     ccAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc                                                                                                                              <  5:159108/133‑1 (MQ=255)                                     ccAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc                                                                                                                              >  6:159108/1‑133 (MQ=255)                                      cAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCtt                                                                                                             >  7:240831/1‑149 (MQ=255)                                      cAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCtt                                                                                                             >  5:315891/1‑149 (MQ=255)                                       aGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTc                                                                                                            <  8:240831/149‑1 (MQ=255)                                       aGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTc                                                                                                            <  6:315891/149‑1 (MQ=255)                                           ccAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                                                        >  4:192063/1‑149 (MQ=255)                                               gACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCg                                                                                                    >  3:102512/1‑149 (MQ=255)                                                 ccAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGCTAt                                                                                                                                         <  2:39608/110‑1 (MQ=39)                                                 ccAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGCTAt                                                                                                                                         >  1:39608/1‑110 (MQ=39)                                                  cagcagGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAg                                                                                                  >  5:6776/1‑148 (MQ=255)                                                                gCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAACGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcggc                                                                                   <  1:313922/149‑1 (MQ=255)                                                                gCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcggc                                                                                   >  1:50240/1‑149 (MQ=255)                                                                 cAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcg                                                                                  >  2:118737/1‑149 (MQ=255)                                                                   gCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcgcg                                                                                >  2:6781/1‑149 (MQ=255)                                                                    cTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGAc                                                                                                                           >  4:192831/1‑105 (MQ=255)                                                                    cTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGAc                                                                                                                           <  3:192831/105‑1 (MQ=255)                                                                     tAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcgcgg                                                                               >  8:47797/1‑148 (MQ=255)                                                                       aGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcgcggc                                                                              <  3:261743/147‑1 (MQ=255)                                                                           aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTg                                                                        >  1:171606/1‑149 (MQ=255)                                                                           aaCTGCAGGCCAGCGTGGCACTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTg                                                                        <  2:50240/149‑1 (MQ=255)                                                                            aCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGc                                                                       <  7:163043/149‑1 (MQ=255)                                                                            aCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGc                                                                       <  2:171606/149‑1 (MQ=255)                                                                                cAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTAc                                                                   <  1:6781/149‑1 (MQ=255)                                                                                 aGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTAc                                                                   <  5:254118/148‑1 (MQ=255)                                                                                 aGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTAc                                                                   >  6:254118/1‑148 (MQ=255)                                                                                   gCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAg                                                                >  6:163284/1‑149 (MQ=255)                                                                                      aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGAGCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGccc                                                             <  6:217487/149‑1 (MQ=255)                                                                                            gCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCaaa                                                       <  3:192063/149‑1 (MQ=255)                                                                                              ccTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgc                                                     >  4:29012/1‑149 (MQ=255)                                                                                              ccTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgc                                                     >  1:35994/1‑149 (MQ=255)                                                                                                 ttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGct                                                  >  1:126251/1‑149 (MQ=255)                                                                                                  tACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCTTCACTTTGCCTACCAGCCCGTCAAAgcgc                                                   <  7:47797/147‑1 (MQ=255)                                                                                                          cTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGc                                         <  4:102512/149‑1 (MQ=255)                                                                                                                  gTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATc                                 <  3:226037/149‑1 (MQ=255)                                                                                                                   tggctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCa                                <  5:163284/149‑1 (MQ=255)                                                                                                                    ggctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAg                               >  6:88200/1‑149 (MQ=255)                                                                                                                     gctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAgg                              >  6:216455/1‑149 (MQ=255)                                                                                                                         gcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgcgc                           >  3:13518/1‑148 (MQ=255)                                                                                                                          cAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgcgc                           >  5:261365/1‑147 (MQ=255)                                                                                                                                 aTAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGc                  >  2:201496/1‑149 (MQ=255)                                                                                                                                     cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCa              >  6:162440/4‑149 (MQ=255)                                                                                                                                     cctgACGCCCTAGCGCGTCGGGCTATCCGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgc                             <  7:193037/131‑1 (MQ=255)                                                                                                                                     cctgACGCCCTAGCGCGTCGGGCTATCCGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgc                             >  8:193037/4‑134 (MQ=255)                                                                                                                                     cccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGc                      >  1:95509/1‑141 (MQ=255)                                                                                                                                     cccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGc                      <  2:95509/141‑1 (MQ=255)                                                                                                                                       cGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATg            <  1:118737/149‑1 (MQ=255)                                                                                                                                       cGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATg            <  7:34538/149‑1 (MQ=255)                                                                                                                                           gcgcTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGttt        <  6:261365/149‑1 (MQ=255)                                                                                                                                                 gggCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGAt  >  2:56125/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGAT  >  NC_002947/4757996‑4758288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑