| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,779,409 | T→G | 10.0% | intergenic (‑143/+18) | pvdD ← / ← pvdJ | non‑ribosomal peptide synthetase/non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,779,409 | 0 | T | G | 10.0% | 142.4 / 7.0 | 50 | intergenic (‑143/+18) | pvdD/pvdJ | non‑ribosomal peptide synthetase/non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base T (21/24); new base G (3/2); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCAAGG > NC_002947/4779269‑4779557 | gAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCggg < 1:199642/147‑1 (MQ=255)gAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATCCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCggg < 1:11005/147‑1 (MQ=255) aTCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCa < 5:24415/149‑1 (MQ=255) aTCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCa < 1:127283/149‑1 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAgc < 8:33643/148‑1 (MQ=255) cAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTATGACCGGGCCCgg < 4:295435/139‑2 (MQ=255) cAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTATGACCGGGCCCgg > 3:295435/1‑138 (MQ=255) aGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACt < 1:10985/149‑1 (MQ=255) aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGt > 1:13031/1‑148 (MQ=255) tCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtc > 5:75772/1‑149 (MQ=255) gCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCg < 1:98200/118‑1 (MQ=255) gCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCg > 2:98200/1‑118 (MQ=255) cGGAACGTGCCAGTCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGAtctc < 3:27219/148‑1 (MQ=255) cGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATctcc > 5:85452/1‑149 (MQ=255) gAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATctcctc > 6:141101/1‑149 (MQ=255) aaCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCctct > 2:85312/1‑149 (MQ=255) aCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTg < 7:194720/149‑1 (MQ=255) ccGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACTGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACg < 1:85312/149‑1 (MQ=255) cTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg < 5:141101/149‑1 (MQ=255) atgatgCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcggc < 6:85452/148‑1 (MQ=255) tgcCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGAtt > 8:284085/1‑149 (MQ=255) ccGAACAAATGAAAGAGCCGGACGGCTGCTGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa < 5:152281/149‑1 (MQ=255) ccGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGAtt > 4:111196/1‑147 (MQ=255) gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGt > 1:170410/1‑126 (MQ=255) gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGt < 2:170410/126‑1 (MQ=255) gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTatc > 5:253249/1‑147 (MQ=255) aaaTGAAAGAGCCGGACGGCTGCCGGCTATGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCACTGCAGTAAGACGTTGCAAGGCGGCCAAGGATTTAGCCaa > 7:300516/1‑148 (MQ=255) agagCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCt < 3:111196/149‑1 (MQ=255) tGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTCTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgcc > 8:7935/1‑149 (MQ=255) gtgtACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCaa > 1:288124/1‑149 (MQ=255) gtacgtaaGTTGACGAGCCGCGCCCGGTCATACCGGGCCCGCAGAGCTCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAaca < 4:190203/143‑1 (MQ=255) tACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAaca < 2:13031/148‑1 (MQ=255) cTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACt < 2:80864/148‑1 (MQ=255) cTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACt > 1:80864/1‑148 (MQ=255) cTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTCTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACt < 7:7935/148‑1 (MQ=255) tGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCCCAGAGCGCTACTGGGTCAACACGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTGATGAAGCGACGCGACCAGCCCGTGCAACACttc > 8:120322/1‑147 (MQ=255) aaGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGa > 1:172304/1‑149 (MQ=255) tGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGc > 8:244427/1‑149 (MQ=255) gACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa < 7:37026/98‑1 (MQ=255) gACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa > 8:37026/1‑98 (MQ=255) gACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa < 7:42044/98‑1 (MQ=255) gACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa > 8:42044/1‑98 (MQ=255) tctgcGGGCCCGGTATGACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgcg < 7:134898/115‑1 (MQ=255) tctgcGGGCCCGGTATGACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgcg > 8:134898/5‑119 (MQ=255) tctgcGGGCCCGGTATGACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCg > 8:49130/5‑149 (MQ=255) aGCCGGGCCCGGTCATACCGGTCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACTTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTTCAACACTGCGACGGCCTCGc < 7:120322/149‑1 (MQ=255) gggCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGaa < 7:244427/149‑1 (MQ=255) gTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTcc > 1:16672/1‑148 (MQ=255) tCATACCGGGCCCGCAGAGCGCTACTGGTTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc < 5:59083/105‑1 (MQ=255) tCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc > 6:59083/1‑105 (MQ=255) tACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCAAgg < 6:253249/149‑1 (MQ=255) | GAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCAAGG > NC_002947/4779269‑4779557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |