Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 358,090 T→C 12.2% intergenic (+58/‑72) tdcG‑I → / → PP_5441 L‑serine dehydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947358,0900TC12.2% 118.3 / 8.7 49intergenic (+58/‑72)tdcG‑I/PP_5441L‑serine dehydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base C (3/3);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

CGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGG  >  NC_002947/357943‑358237
                                                                                                                                                   |                                                                                                                                                   
cGCCCCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgtg                                                                                                                                                    <  4:264249/149‑1 (MQ=255)
cGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgtg                                                                                                                                                    <  8:56211/149‑1 (MQ=255)
cGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgtg                                                                                                                                                    <  6:202723/149‑1 (MQ=255)
                ccGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGc                                                                                                                                    <  3:298201/149‑1 (MQ=255)
                ccGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTCAAGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGc                                                                                                                                    <  1:209025/149‑1 (MQ=25)
                   gcgcCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCAGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCggg                                                                                                                                 <  5:320592/149‑1 (MQ=25)
                      ccGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                              <  2:89349/149‑1 (MQ=255)
                        gATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTCCAATCCCTGTAGGAGCGGGCTTGc                                                                                                                            <  1:35645/149‑1 (MQ=255)
                             gcccGACATGTACAAAGAGACGGCGCGCGGCGGCCTGGCGGTCAGCGCTATGGTGTGCTGATTTTCTGAGCTGACCTTCTGGGCGTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTTGGAGCGGGCTTGCCcgc                                                                                                                        <  4:309203/145‑1 (MQ=38)
                              cACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCcgcg                                                                                                                       >  8:91115/1‑148 (MQ=255)
                                 gACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGaa                                                                                                                     >  5:312267/1‑147 (MQ=255)
                                    aaGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCt                                                                                                                                            >  1:272322/1‑121 (MQ=255)
                                    aaGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCt                                                                                                                                            <  2:272322/121‑1 (MQ=255)
                                      gTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGccc                                                                                                              >  6:260809/1‑149 (MQ=255)
                                       tACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCAACTACAGGAACTGTGCAATCCCGGTAGGAGCGGGCTTGCCCGCGAAGAGGccca                                                                                                             >  7:19416/1‑148 (MQ=255)
                                         aaaagttaCCTCGCGCGGGGGCCTGGCGTGCTGCGCTATCGAGGGCTGTTTTGCTTAGCTGACCTTCTGGCCCTCTTCTCGTGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTATGTGCGGGCTTGCCCGCGAAGAGGCCCTTg                                                                                                           <  6:241613/142‑1 (MQ=25)
                                            cgagcCCTCCCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTCCTGATTTTCTGACCTCCCCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGCATCCGTGCAACCCCTCTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAgg                                                                                                        <  1:76472/148‑1 (MQ=37)
                                            agagACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCggg                                                                                                                                 >  3:157551/1‑124 (MQ=255)
                                            agagACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCggg                                                                                                                                 <  4:157551/124‑1 (MQ=255)
                                               gaCCTCGCGCGGCGGCCGGGCGCTCAGCGCTAGCGCGTGCTGATTTTCTGAGCGGACCTGCTGGCCCTCTGCGCGGGCAAGCCCGCTCCTACAGCACCTGTGCAATCCCTGTCGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTg                                                                                                     <  5:144783/149‑1 (MQ=37)
                                                    cgcgcgGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCGGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATg                                                                                                  >  8:63418/1‑147 (MQ=255)
                                                     gcgcgGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGCTTTTCTGAGCTGCCCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTCGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAg                                                                                               <  5:307896/149‑1 (MQ=255)
                                                     gcgcgGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGCTTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAg                                                                                               <  5:312603/149‑1 (MQ=255)
                                                          gcggcCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGc                                                                                           >  6:304916/1‑148 (MQ=255)
                                                                 ggCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGAGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAAc                                                                                   >  6:134049/1‑149 (MQ=255)
                                                                        aGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTa                                                                             <  5:304916/148‑1 (MQ=255)
                                                                        aGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTAt                                                                            <  5:260809/149‑1 (MQ=255)
                                                                          cgcTATCGTGTGCTTTTTTTGTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTAAAGCAACTGTGCAATCCCTGTTGGAGGGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGc                                                                          <  8:25219/149‑1 (MQ=37)
                                                                            cTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCct                                                                        >  2:93549/1‑149 (MQ=255)
                                                                               tCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCtt                                                                     >  2:230239/1‑149 (MQ=255)
                                                                                 gAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCaa                                                                                         <  4:263206/127‑1 (MQ=255)
                                                                                 gAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCaa                                                                                         >  3:263206/1‑127 (MQ=255)
                                                                                      cTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTACTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTg                                                              >  8:93763/1‑149 (MQ=255)
                                                                                            ttCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGc                                                                                                                                                   <  7:198306/58‑1 (MQ=255)
                                                                                            ttCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGc                                                                                                                                                   >  8:198306/1‑58 (MQ=255)
                                                                                                        cTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGc                                            <  7:91115/149‑1 (MQ=255)
                                                                                                        atgcTGGCCCCCTTCGCGGCCACCCCCGCCCAAACACCACCTGTCCACCCCCTGTAGGAGCGGGCTTCCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGc                                            <  5:15488/146‑1 (MQ=255)
                                                                                                              ggCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTAc                                        <  7:63418/147‑1 (MQ=255)
                                                                                                                  tctTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcg                                  >  5:93235/1‑149 (MQ=255)
                                                                                                                     tCGCGGGCAAGCCCGCTCCTACATGAACTGTGCAATCCCTGTTGGAGGGGGCTTGCCCGCGAATAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgtc                                <  4:222216/148‑1 (MQ=255)
                                                                                                                       gcgGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCgg                              <  6:312267/148‑1 (MQ=255)
                                                                                                                        cgGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCCACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGAt                            >  2:201536/1‑149 (MQ=255)
                                                                                                                                cccGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt                    >  5:51891/1‑149 (MQ=37)
                                                                                                                                cccGCTCCAACCGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt                    <  7:11845/149‑1 (MQ=255)
                                                                                                                                  cGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCgg                                                           >  1:42527/1‑108 (MQ=21)
                                                                                                                                  cGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCgg                                                           <  2:42527/108‑1 (MQ=21)
                                                                                                                                  cGCTCCTACAGGAACTGTGCAATCCCTGTCGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCa                  <  6:93235/149‑1 (MQ=255)
                                                                                                                                       cTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg             >  7:255420/1‑149 (MQ=37)
                                                                                                                                       cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg             >  3:162088/1‑149 (MQ=255)
                                                                                                                                       cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg             >  1:116454/1‑149 (MQ=255)
                                                                                                                                          cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTgg            <  5:134049/147‑1 (MQ=255)
                                                                                                                                          cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGCTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGc          >  6:264340/1‑149 (MQ=255)
                                                                                                                                                   tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCgg  <  7:93763/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGG  >  NC_002947/357943‑358237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑