| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,858,702 | C→G | 8.3% | intergenic (‑208/+17) | PP_mr50 ← / ← ligA | Bacteria_large_SRP ncRNA/DNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,858,702 | 0 | C | G | 8.3% | 150.9 / 3.1 | 48 | intergenic (‑208/+17) | PP_mr50/ligA | Bacteria_large_SRP ncRNA/DNA ligase |
| Reads supporting (aligned to +/- strand): ref base C (25/19); new base G (2/2); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
ATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATGGGTCTTGC > NC_002947/4858557‑4858849 | aTACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAgg > 7:231258/1‑149 (MQ=255) tACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCATGTTTTGAGAAGACAGTTGCCCCCCACAggg < 1:27739/149‑1 (MQ=255) catcatTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTAt > 4:176991/1‑149 (MQ=255) atTTCTCAATTACTTGTCAGGGTCGAGATGGCCTGGACGTGCCTCTTCGCGTGTAAACCCGCTCCTACTGTCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGcc < 5:200577/148‑1 (MQ=39) ctcAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAGCCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAgg > 6:297968/1‑116 (MQ=255) aaTGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCt < 4:39142/128‑1 (MQ=255) aaTGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCt > 3:39142/1‑128 (MQ=255) aaTGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCgg > 8:271746/1‑149 (MQ=255) gttGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAg < 6:202784/147‑1 (MQ=255) tCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCAACAGGGGGGCACGGATGCCTAGAACGCTATGCCCTGTTCGGCCAGGAATGTGACa > 1:120365/1‑148 (MQ=255) ccTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCt > 6:104814/1‑148 (MQ=255) cTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAggggg > 6:76957/1‑91 (MQ=255) cTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTc > 7:111711/1‑149 (MQ=255) cTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTc < 8:231258/149‑1 (MQ=255) gACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTc > 3:69371/1‑149 (MQ=255) cgcgGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACaaa < 2:324156/125‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACaaa > 1:324156/1‑125 (MQ=255) cgcgGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTca < 3:176991/148‑1 (MQ=255) gcgGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTcaca > 5:6872/1‑149 (MQ=255) aaaCCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGcc > 3:232685/1‑94 (MQ=255) aaaCCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGcc < 4:232685/94‑1 (MQ=255) cccGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCg < 8:111711/149‑1 (MQ=255) ccGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCg < 7:18626/124‑1 (MQ=255) ccGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCg > 8:18626/1‑124 (MQ=255) ccGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCgg > 2:147017/1‑149 (MQ=255) ccGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCgg > 4:8008/1‑149 (MQ=255) ccGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCgg > 1:221012/1‑149 (MQ=255) cTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCt < 7:271746/149‑1 (MQ=255) cGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTgg < 1:93212/149‑1 (MQ=255) cGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTgg > 2:93212/1‑149 (MQ=255) gggCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGc < 6:6872/149‑1 (MQ=255) cgcgCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTc < 2:221012/149‑1 (MQ=255) ttttCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGcc > 8:55714/1‑149 (MQ=255) cAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAg > 6:98142/1‑149 (MQ=255) aaGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg > 8:35887/1‑149 (MQ=255) ttttGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg < 5:142377/146‑1 (MQ=255) ttttGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg > 6:142377/1‑146 (MQ=255) ttGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCa < 7:55714/148‑1 (MQ=255) gaagaCAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGccac > 3:130994/1‑149 (MQ=255) aagaCAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg < 7:184751/139‑1 (MQ=255) aagaCAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg > 8:184751/1‑139 (MQ=255) ccgtGCCCCCCTGTGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg > 7:206281/4‑134 (MQ=255) ccgtGCCCCCCTGTGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAgg < 8:206281/131‑1 (MQ=255) cgtGCCCCCCTGTGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTgg < 2:216443/109‑1 (MQ=255) cgtGCCCCCCTGTGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTgg > 1:216443/3‑111 (MQ=255) gCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATgg > 1:217780/1‑149 (MQ=255) ccccccACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATgg < 3:8008/148‑1 (MQ=255) cccACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGgccgcc < 2:29720/100‑1 (MQ=255) acaGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATGGGTCTTGc > 2:196609/1‑149 (MQ=255) acaGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATGGGTCTTGc < 4:69371/149‑1 (MQ=255) acaGGGGGGCACGGATGCCTAGAACGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATGGGTCTTGc < 2:120365/149‑1 (MQ=255) | ATACAACCACTGCGGCAGAAAAAGTCATCATTTCTCAATGACTTGTCAGGGTCGAGATGGCCTGGACGGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGACCGCGCAGGCTTTTCAAGTTTTGAGAAGACAGTTGCCCCCCACAGGGGGGCACGGATGCCTAGACCGCTATGCCCTGTTCGGCCAGGAATGTGACAAAGGCATCTTCGTCCAGCACCTTCACACCCAACTCGGCCGCCTTGGCCAGCTTCGAGCCTGCCCCAGGCCCAGCCACCACGCAATGGGTCTTGC > NC_002947/4858557‑4858849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |