| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,399 | T→C | 10.0% | intergenic (+32/‑176) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,399 | 0 | T | C | 10.0% | 101.0 / 3.0 | 42 | intergenic (+32/‑176) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (18/18); new base C (2/2); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
CCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCC > NC_002947/4896255‑4896546 | ccATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGc > 7:186547/1‑149 (MQ=255) gCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGGTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTcc > 8:253164/1‑148 (MQ=255) acgttgcgtccttgttcttgaagggtgttggggcagattgccagcgactataacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgcGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGcgcg < 3:176819‑M1/39‑1 (MQ=255) gtccttgttcttgaagggtgttggggcagattgccagcgactataacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GGCCTCTTCGCGGGTAAACCCGCTCCTACAGGCGCGGACCGTg < 7:117620‑M1/46‑1 (MQ=255) caTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCTCTGTGGGAATGTGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTAGCGGGTAATTACCCTACAACAGGCGCTGGCCGTgcgc > 5:228533/1‑149 (MQ=255) caTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTgcgc > 7:108197/1‑149 (MQ=255) aTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGcgcg < 8:108197/149‑1 (MQ=255) gCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTcc‑‑‑c < 8:264064/148‑1 (MQ=255) aagggtgttggggcagattgccagcgactataacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GGCCTCTTCGCGGGTAAACCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCg > 8:117620‑M1/91‑148 (MQ=255) ccTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAgg < 4:145464/149‑1 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGCCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCgg < 7:253164/149‑1 (MQ=255) agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt > 8:186547‑M2/1‑146 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGAtttacccgc < 5:1442‑M2/148‑10 (MQ=255) cACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGGCCAATTCCCGGGTAAAACCCGACAAACCGGCCCGGACCGTGCCCGGACC‑‑‑CCCGTAGGAGCGGGTTTAccccc > 2:223560/1‑146 (MQ=255) cGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGa > 8:96086/1‑109 (MQ=255) cGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGa < 7:96086/109‑1 (MQ=255) cGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCggg < 8:174384/136‑1 (MQ=255) cGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCggg > 7:174384/1‑136 (MQ=255) cGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCGCGCACGGTCCGCGCCTGTAGGAGCGGAtttacccgcgaag > 6:1442‑M2/1‑136 (MQ=255) gcgactataacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgcGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAaga > 4:176819‑M1/68‑149 (MQ=255) ctataacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAc < 8:169467‑M1/73‑1 (MQ=255) ctataacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTAc > 7:169467‑M1/64‑136 (MQ=255) gggggCGGCGTTCTGTTCGCGCTGGGGCAAGGGGTTGTTCCCCAAAGCGTGATAGCGCCTGCGCG‑GGCCTCTTGGCGGGTAAATCCGCTCCTACCGGCGGGGACGGTGCGCGTTCC‑‑‑CCCTTTGGAGCGGGTTTACCCGCGAAGAGGCCg < 2:308491/149‑1 (MQ=37) taacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGaa > 8:31902‑M1/61‑140 (MQ=255) taacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGaa < 7:31902‑M1/80‑1 (MQ=255) taacgggtgggtttatacgggttaaagaacaaaaaatgctttggttatgcttctgcctgtGCC‑GCCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCg > 2:99861‑M1/61‑147 (MQ=255) ttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCACTTAGCGGGTAAATCCGCTCCTAAAGGCGCGGACCGGGCGCGGTGC‑‑‑CCCGTAGGAGCGGGTTTAACCGCGAAGAGACCGGCACAgg > 1:269850/1‑146 (MQ=255) ctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt > 8:35444‑M2/1‑118 (MQ=255) ctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt < 7:35444‑M2/121‑4 (MQ=255) gttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACTGGCGCGGACCGTGCGCGGGCC‑‑‑CCCGTAGGAGCGGGtttacccgcgaagaggccggc > 6:251500‑M2/1‑116 (MQ=255) gttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGtttacccgcgaagaggccggc < 5:251500‑M2/137‑22 (MQ=255) aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCACATAGCCGGTAAATACGCTACAAAACGCCCGGACCCTGCCCGGTCC‑‑‑CCCCTACGAGCGGGTTTAACCCCCAAGAGGCCGGCACAAGCAAAAGC‑AAAACCAAAACaat > 6:297037/1‑147 (MQ=255) tcgcAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTCtt < 5:297037/145‑1 (MQ=255) ccAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttta < 5:305389‑M2/93‑5 (MQ=255) ccAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttta > 6:305389‑M2/1‑89 (MQ=255) cAAAGCGTGATAGCGCCTGCGCC‑GGCCTAATAGCGGGTAAAAACCCACCAACAGGGGCGGGCCCGGGGCGGTGC‑‑‑CCCGGATGGGCGGGGTTTACAGCCAAAAGGCCCGCACAAAGAGAAGCAAAAACCAAAAAATtttttttttata > 8:14087/1‑143 (MQ=255) cAAAGCGGTATAGCGCCTGAGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGCCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGTAGCGGGTTTACCCGCTAAGAGGCCGGCACAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTc < 7:14087/143‑1 (MQ=38) aaaGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTCTTTAAcc > 7:164824/1‑149 (MQ=255) cgcC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGt < 1:223560/148‑1 (MQ=255) GCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTCTTTAGCCCGTAATAACCCACGCGTTATAGt < 5:267789‑M1/149‑1 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGtttacccgcgaagaggccggcacaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtctt < 1:319028‑M2/149‑98 (MQ=255) ATCCGCTCCTACAGGCGCTGACCGTTCGCGGTCC‑‑‑CCCGTAGTAGCGGGTTTACCCGCGAAGAGGCCGTCACAGGCAGAAGC‑ATAACCAAACCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGccc > 6:228533‑M1/1‑149 (MQ=255) tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGAtttacccgcgaagaggccggcgcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgt < 2:168079‑M2/149‑103 (MQ=255) | CCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCC‑GGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGC‑ATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCC > NC_002947/4896255‑4896546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |