Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 362,956 C→T 7.5% G176D (GGC→GAC)  lcdH ← L‑carnitine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947362,9560CT 7.5% 152.4 / 2.6 53G176D (GGC→GAC) lcdHL‑carnitine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base T (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTCGACGACTCG  >  NC_002947/362808‑363099
                                                                                                                                                    |                                                                                                                                                
gCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGc                                                                                                                                                  >  5:293981/1‑149 (MQ=255)
       ccAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGc                                                                                                                                           <  8:170660/149‑1 (MQ=255)
        cAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCg                                                                                                                                          >  3:211583/1‑149 (MQ=255)
                aTCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGAt                                                                                                                                  <  8:233685/149‑1 (MQ=255)
                 tCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATg                                                                                                                                 >  8:99021/1‑149 (MQ=255)
                      tcgtcgATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGtttt                                                                                                                            >  1:145195/1‑149 (MQ=255)
                      tcgtcgATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGtttt                                                                                                                            <  8:40063/149‑1 (MQ=255)
                        gtcgATTTCGCCAGTGGTGGCAACTCCGTCGTTGAACAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTg                                                                                                                           >  5:242774/1‑148 (MQ=255)
                            aTTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAg                                                                                                                       <  6:242774/148‑1 (MQ=255)
                            aTTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGc                                                                                                                      >  3:174728/1‑149 (MQ=255)
                            aTTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGc                                                                                                                      <  8:128037/149‑1 (MQ=255)
                             tttCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCt                                                                                                                     <  6:293981/149‑1 (MQ=255)
                                    cGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGTCGAGTGCGGTGTAGATGGTTTTGGCAGCtt                                                                                                                    <  1:46089/143‑1 (MQ=255)
                                    cGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGTCGAGTGCGGTGTAGATGGTTTTGGCAGCtt                                                                                                                    >  2:46089/1‑143 (MQ=255)
                                    cGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGTCGAGTGCGGTGTAGATGGTTTTGGCAGCtt                                                                                                                    >  8:137062/1‑143 (MQ=255)
                                    cGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGTCGAGTGCGGTGTAGATGGTTTTGGCAGCtt                                                                                                                    <  7:137062/143‑1 (MQ=255)
                                             aaCTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGt                                                                                                      <  8:182896/148‑1 (MQ=255)
                                             aaCTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGt                                                                                                      >  6:89394/1‑148 (MQ=255)
                                             aaCTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGt                                                                                                      >  7:182896/1‑148 (MQ=255)
                                              aCTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGt                                                                                                      >  3:59812/1‑147 (MQ=255)
                                                tCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCa                                                                                                                  <  4:241112/133‑1 (MQ=255)
                                                tCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCa                                                                                                                  >  3:241112/1‑133 (MQ=255)
                                                   gtcgtTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGc                                                                                               >  2:167072/1‑149 (MQ=255)
                                                   gtcgtTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGc                                                                                               <  5:305263/149‑1 (MQ=255)
                                                       ttGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCt                                                                                           <  7:99021/149‑1 (MQ=255)
                                                         gACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTAc                                                                                         >  4:29787/1‑149 (MQ=255)
                                                          aCCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTAcc                                                                                        >  5:10213/1‑149 (MQ=255)
                                                          aCCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTAcc                                                                                        >  6:143254/1‑149 (MQ=255)
                                                                  cAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCcgacga                                                                                >  2:177184/1‑149 (MQ=255)
                                                                    aGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAt‑c                                                                             <  5:261641/149‑1 (MQ=255)
                                                                        cTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGTTGCGGCTACCGCCGACGAT‑CTcca                                                                         >  4:219844/1‑149 (MQ=255)
                                                                         tCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCg                                                                                                                                          <  8:219545/84‑1 (MQ=255)
                                                                         tCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCg                                                                                                                                          >  7:219545/1‑84 (MQ=255)
                                                                         tCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTccac                                                                        >  3:257736/1‑149 (MQ=255)
                                                                              ccAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCg                                                                   <  4:59812/149‑1 (MQ=255)
                                                                                  agCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGTTTTTGGCAGCTTCAATAGCTTTGTGTTAGGTGCGGCTACCGACGACGAT‑CTCCACCAGCGgca                                                                <  6:65404/148‑1 (MQ=255)
                                                                                   gcgcTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGgcagc                                                              <  1:209923/149‑1 (MQ=255)
                                                                                       ttCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCgg                                                                                                        >  7:31571/1‑104 (MQ=255)
                                                                                       ttCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCgg                                                                                                        <  8:31571/104‑1 (MQ=255)
                                                                                            gCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACt                                                     >  5:222207/1‑149 (MQ=255)
                                                                                               ggCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGATCCTCCACCAGCGGCAGCAGGTAAACTgg                                                   <  1:167072/149‑1 (MQ=255)
                                                                                                 cGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGTCAGCTTCAATATTTTTTTTTTATGTGAGGGTAACGACGTAGAT‑CTACACCAGCGGCAGCAGGTAAACTGGGtt                                                <  4:174728/149‑1 (MQ=255)
                                                                                                         gATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGT‑AACTGGGTTGAACGGGTg                                       <  1:177184/149‑1 (MQ=255)
                                                                                                                                gcACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTg                                                    <  1:218176/114‑1 (MQ=255)
                                                                                                                                gcACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTg                                                    >  2:218176/1‑114 (MQ=255)
                                                                                                                                 cacaTGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCTCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGt                <  5:89394/149‑1 (MQ=255)
                                                                                                                                   cctGCCGCGGGCGCATGCCGAGTCCGGTGTAGATGCTTTTGGCAGCTTCAATAGCTTCTGTTGAGGTTCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGa              <  8:292954/147‑1 (MQ=255)
                                                                                                                                    aTGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGt                                                 >  4:259571/1‑113 (MQ=255)
                                                                                                                                    aTGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGt                                                 <  3:259571/113‑1 (MQ=255)
                                                                                                                                       cAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGTTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTc           <  6:10213/148‑1 (MQ=255)
                                                                                                                                        aGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTcga         >  1:129399/1‑149 (MQ=255)
                                                                                                                                            ggCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTCgacgac     <  4:257736/149‑1 (MQ=255)
                                                                                                                                             gcgcATGCCGTGTGTGTTGTAGCTGGGTTTGGCAGCTTCAATTTTTTTTTTTTATGTTCGGTTTCCGACGAAGAT‑CTCCACCAGCGGAAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTCGACGACt    <  8:289648/149‑1 (MQ=255)
                                                                                                                                                 aTGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTCGACGACTCg  >  7:265975/1‑147 (MQ=255)
                                                                                                                                                    ccGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTg                                       <  1:151718/107‑1 (MQ=255)
                                                                                                                                                    ccGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTg                                       >  2:151718/1‑107 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAGCAGGCGGTCGGCGATGAAGCCTGGCACTTCCTTGCGCACATGCAGCGGGCGCATGCCGAGTGCGGTGTAGATGGTTTTGGCAGCTTCAATAGCCTCGGGTGAGGTGCGGCTACCGCCGACGAT‑CTCCACCAGCGGCAGCAGGTAAACTGGGTTGAACGGGTGGCCGACGACGCAGCGTTCAGGGTGAGTCGACGACTCG  >  NC_002947/362808‑363099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑