Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,930,410 G→C 7.1% T188R (ACA→AGA) ‡ cheZ ← protein phosphatase CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,930,4100GC 7.1% 218.3 / 6.2 70T188R (ACA→AGA) ‡cheZprotein phosphatase CheZ
Reads supporting (aligned to +/- strand):  ref base G (33/32);  new base C (2/3);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATTCC  >  NC_002947/4930265‑4930556
                                                                                                                                                 |                                                                                                                                                   
cGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtgg                                                                                                                                                  <  2:235862/149‑1 (MQ=255)
 ggACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtggt                                                                                                                                                 >  1:203240/1‑149 (MQ=255)
  gACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtggt                                                                                                                                                 >  2:237603/1‑148 (MQ=255)
     cTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAcc                                                                                                                                             <  3:221624/149‑2 (MQ=255)
       tCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGAcgcg                                                                                                                                           >  2:195949/1‑149 (MQ=255)
         aCCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACgcgc                                                                                                                                          <  1:292958/148‑1 (MQ=255)
          ccccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCtt                                                                                                                                        <  1:298590/149‑1 (MQ=255)
           cccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCtt                                                                                                                                        >  7:275865/1‑148 (MQ=255)
           cccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGCTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTg                                                                                                                                       <  2:172104/149‑1 (MQ=255)
            ccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGa                                                                                                                                      >  8:242754/1‑149 (MQ=255)
             cGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAACACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGAt                                                                                                                                     <  6:49974/149‑1 (MQ=255)
                   ggATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCt                                                                                                                                <  5:142282/148‑1 (MQ=255)
                   ggATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCt                                                                                                                                >  6:142282/1‑148 (MQ=255)
                   ggATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTg                                                                                                                               <  8:275865/149‑1 (MQ=255)
                    gATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTgg                                                                                                                              <  4:150368/149‑1 (MQ=255)
                     aTGCTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGc                                                                                                                             <  5:35507/149‑1 (MQ=255)
                        tttttCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCg                                                                                                                           <  7:242754/148‑1 (MQ=255)
                          tttCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCTGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGAAGGTTGCTTTCCACATCGGTCACAACACTGGTGAcagag                                                                                                                                          >  6:329718/1‑127 (MQ=255)
                          tttCTCGATCTTTTTCTGCACGCAATTGATCGTGGCCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAccgag                                                                                                                                          <  5:329718/131‑5 (MQ=255)
                            tctcGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGAGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCAACTCGTTCACCAGTGTGGTGAAGCGCTTGATCACCTGGCCGGTCAg                                                                                                                      >  7:211753/1‑149 (MQ=255)
                                 aTCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCTGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCt                                                                                                                 >  3:295259/1‑149 (MQ=255)
                                  tCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACAAGCTTGAGCAGGTTGATTTCCACCTCGGTAACCAGTGTGGTGACGCGCTTTATCACCTGGCAGGTCAGGTCCt                                                                                                                 >  5:93190/1‑148 (MQ=255)
                                    tttttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTgc                                                                                                               >  4:64078/1‑147 (MQ=255)
                                      tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAg                                                                                                            >  4:203114/1‑149 (MQ=255)
                                            cACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCACGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTgg                                                                                                      >  2:99122/1‑149 (MQ=255)
                                               gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                    >  2:196045/1‑148 (MQ=255)
                                                 aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAgg                                                                                                                     <  2:301725/129‑1 (MQ=255)
                                                 aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAgg                                                                                                                     >  1:301725/1‑129 (MQ=255)
                                                 aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTc                                                                                                          <  1:116537/140‑1 (MQ=255)
                                                 aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTc                                                                                                          >  2:116537/1‑140 (MQ=255)
                                                           ggTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGt                                                                                         <  1:234307/147‑1 (MQ=255)
                                                           ggTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGt                                                                                         >  2:234307/1‑147 (MQ=255)
                                                               aTGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGtt                                                                                     >  6:228443/1‑147 (MQ=255)
                                                               aTGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTg                                                                                    >  2:161245/1‑148 (MQ=255)
                                                               aTGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTg                                                                                    <  1:161245/148‑1 (MQ=255)
                                                                tGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAg                                                                                  <  3:203114/149‑1 (MQ=255)
                                                                tGTTTTATACCAGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAg                                                                                  >  1:82749/1‑149 (MQ=255)
                                                                       taCCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGGCGTTGAGGTGCCCg                                                                           <  2:122820/149‑1 (MQ=255)
                                                                         ccGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCgg                                                                          >  3:73192/1‑148 (MQ=255)
                                                                         ccGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCgg                                                                          <  1:237603/148‑1 (MQ=255)
                                                                                aGCGGTCGACCGGGCTGGCCATCAGCACCAGCTTGAGGAGGGTGCTTTCCACCTCGGTCACCATTGTGGTGACGCTCTTGCTCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGGCGTTGAGGTGCCCGGAAACCTTg                                                                  <  6:93190/149‑1 (MQ=255)
                                                                                 gCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCg                                                                           <  7:145086/139‑1 (MQ=255)
                                                                                 gCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCg                                                                           >  8:145086/1‑139 (MQ=255)
                                                                                   ggTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCgg                                                               <  3:260517/149‑1 (MQ=255)
                                                                                       gACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTAc                                                           >  1:122493/1‑149 (MQ=255)
                                                                                            ggctggcCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑g                                                     >  1:142702/1‑149 (MQ=255)
                                                                                                  ccATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCtg                                               >  4:126439/1‑149 (MQ=255)
                                                                                                     tCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑g                                                     <  8:224134/140‑1 (MQ=255)
                                                                                                     tCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑g                                                     >  7:224134/1‑140 (MQ=255)
                                                                                                          aCCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTg                                                                  <  3:209153/123‑1 (MQ=255)
                                                                                                           ccAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAg                                       >  7:55834/1‑148 (MQ=255)
                                                                                                            cAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCtgt                                              <  5:233200/140‑1 (MQ=255)
                                                                                                            cAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCtgt                                              >  6:233200/1‑140 (MQ=255)
                                                                                                              gCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGaaa                                   >  1:34493/1‑149 (MQ=255)
                                                                                                                   aGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTc                              <  5:228443/149‑1 (MQ=255)
                                                                                                                    gCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTc                              >  8:101407/1‑148 (MQ=255)
                                                                                                                         ttGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCg                        <  3:126439/149‑1 (MQ=255)
                                                                                                                           gCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGc                       <  2:142702/148‑1 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTa                                                            <  7:30368/111‑1 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTa                                                            >  8:30368/1‑111 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCTGGCGCTGTGCGTCAGGAAACTGTCGACTCGc                       <  1:196045/148‑1 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCtt                     <  2:203240/149‑1 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCt                      <  1:195949/148‑1 (MQ=255)
                                                                                                                                ccACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                  <  1:99122/148‑1 (MQ=255)
                                                                                                                                ccACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                  <  2:82749/148‑1 (MQ=255)
                                                                                                                                ccACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                  >  5:60181/1‑148 (MQ=255)
                                                                                                                                 cACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGTGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                  >  1:289688/1‑147 (MQ=255)
                                                                                                                                 cACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAcc                 <  2:34493/148‑1 (MQ=255)
                                                                                                                                   ccTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                  >  8:99903/1‑145 (MQ=255)
                                                                                                                                      gcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTc                                         <  5:295201/117‑1 (MQ=255)
                                                                                                                                      gcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTc                                         >  6:295201/3‑119 (MQ=255)
                                                                                                                                           aCCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGaaa      >  6:286311/1‑149 (MQ=255)
                                                                                                                                           aCCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGaaa      >  8:117791/1‑149 (MQ=255)
                                                                                                                                               gtgtGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATTcc  >  6:10593/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTC‑GGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATTCC  >  NC_002947/4930265‑4930556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑