| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,949,941 | C→G | 6.5% | G135R (GGC→CGC) | fliM ← | flagellar biosynthesis switching/energizing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,949,941 | 0 | C | G | 6.5% | 196.5 / 3.2 | 61 | G135R (GGC→CGC) | fliM | flagellar biosynthesis switching/energizing protein |
| Reads supporting (aligned to +/- strand): ref base C (29/28); new base G (2/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CCTCGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTC > NC_002947/4949797‑4950083 | ccTCGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATc < 8:199579/148‑1 (MQ=255) cTCGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATcgc > 7:240520/1‑149 (MQ=255) cTCGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATcgc > 7:80065/1‑149 (MQ=255) cTCGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGATCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATcgc > 3:274071/1‑149 (MQ=255) cATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATAGCCGCCAAAg > 3:234591/1‑148 (MQ=255) aTGAACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAACCACTGGACCAGCACCATGCGCACCACACGCAGTTCGGTCGCGGTGAACTCACGCCCTTCGATCTTGGCGAGC‑CGCCCAAGGCCCCCaaaca > 3:210440/1‑146 (MQ=255) tACTGGCAGCTGTCGGGGATGCTCGCAGGCGAGGCTTCCTTGAGGTGGACGAAACACTGGTCCAGCTCGATGCGCACCTCAGGCTGTTCGGTCGGGGTGAACTCACGGCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCGAAAGAAAtt < 4:210440/149‑1 (MQ=255) tCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCCGGCCACACCCCCCAaaaaaaatt > 2:163246/1‑145 (MQ=255) gAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTc < 8:240520/147‑1 (MQ=255) aCCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGc < 8:80065/147‑1 (MQ=255) ggCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGaa < 4:45631/149‑1 (MQ=255) gCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAAAACCAGCtt > 3:233060/1‑149 (MQ=255) ccAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGc < 1:163246/147‑1 (MQ=255) cAGGCTTCCTTGAGATCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTc < 4:274071/149‑1 (MQ=255) gCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAg > 6:149555/1‑149 (MQ=255) ccTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGAt < 4:310659/148‑1 (MQ=255) cTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATg > 7:235367/1‑148 (MQ=255) ttGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGaa > 3:81615/1‑149 (MQ=255) ttGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGa > 2:243345/1‑148 (MQ=255) ctGACGTCGACGCACCACTGGTCCAGCACCATGCGCCCCACACGCAGTTCGTGCTGCGGGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGaa < 4:234591/148‑1 (MQ=255) tCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCg > 2:47779/1‑148 (MQ=255) gacgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCGTGGCGTCGAGGATGAACAGCGAAg > 6:60304/1‑149 (MQ=255) gacgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATAGCCGCCAAAGAAATTGTCCACCAGGTTGAACACCCGGTTGTGGTCGAGGATGATCAGGGAAg > 2:180240/1‑149 (MQ=255) aaaCACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGcc > 3:27632/1‑149 (MQ=255) aaaCACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGcc < 4:81615/149‑1 (MQ=255) acacTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCcgc > 8:278650/1‑149 (MQ=255) tGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑GGGCGATGGCCGCCacgc < 8:34860/87‑4 (MQ=255) tGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCGATGGCCGCCacgc > 7:34860/1‑84 (MQ=255) ggTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGaa < 3:68029/137‑1 (MQ=255) gTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAg < 7:278650/147‑1 (MQ=255) gTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGc < 4:233060/148‑1 (MQ=255) tCCAGCACCATGCGCACCACTCGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCcgctgcgcgg < 7:37023/149‑8 (MQ=255) aCCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCa > 6:85850/1‑103 (MQ=255) aCCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCa < 5:85850/103‑1 (MQ=255) ccATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATc < 4:27632/149‑1 (MQ=255) ccATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATc > 8:230690/1‑149 (MQ=255) taccacACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCa < 3:231568/148‑1 (MQ=255) ccacACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAgg < 5:149555/149‑1 (MQ=255) ccacACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCCAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCa > 4:231568/1‑147 (MQ=255) cacaCGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCcgcg < 3:112998/126‑1 (MQ=255) cacaCGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATAGCCGCCAAAGAAAATGTACACCAGCATGAACACCAGCGTGGCGTCGAGGATTAACAGCGCAGTTCcgcg > 4:112998/1‑126 (MQ=255) acacGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGtt < 8:235367/149‑1 (MQ=255) acGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCt < 3:263512/131‑1 (MQ=255) acGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCt > 4:263512/1‑131 (MQ=255) acGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTg > 1:169061/1‑133 (MQ=255) acGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTg < 2:169061/133‑1 (MQ=255) ggTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCATTAACCCCAGCATGGCGTCGAGGATGAACAGCGAAGTGCCGCCCAGCGGCATGAACTTGACCAGGTTGAGGCTGGTGGGCACg > 5:285839/1‑148 (MQ=255) cACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAg > 5:324865/1‑149 (MQ=255) aCGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAACAAAAAGTCCCCCAGCTTGAACACCAGCATGGCCTCGAGGATGAACAGCGAAGAGCCGCGCAGCGGCTTGATCTTGACCAGGTTGACGCTCGTGGGCACGTACAGCGAgg > 2:89475/1‑148 (MQ=255) ccTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTg > 7:189323/1‑146 (MQ=255) ccTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTg < 8:189323/146‑1 (MQ=255) ttCGATCTTGGCGTGG‑CGGCCAACGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACg > 4:38630/1‑148 (MQ=255) ttGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGCGCACGTACAGCGAGTGCACGTATTCGcc < 1:180240/149‑1 (MQ=255) tggcgtgg‑cgGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCtt > 6:130538/1‑100 (MQ=255) tggcgtgg‑cgGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCtt < 5:130538/100‑1 (MQ=255) tttgg‑cgGCGATGGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGc < 6:114525/126‑1 (MQ=255) tttgg‑cgGCGATGGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGc > 5:114525/3‑128 (MQ=255) tgg‑cgGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGt > 6:145512/1‑103 (MQ=255) tgg‑cgGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGt < 5:145512/103‑1 (MQ=255) g‑cggcCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTc < 1:47779/148‑1 (MQ=255) g‑cggcCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTc < 1:243345/148‑1 (MQ=255) | CCTCGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGG‑CGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTC > NC_002947/4949797‑4950083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |