Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,992,731 G→A 7.4% V230I (GTA→ATA)  bkdAA → branched‑chain alpha‑keto acid dehydrogenase complex subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,992,7310GA 7.4% 152.0 / 2.2 54V230I (GTA→ATA) bkdAAbranched‑chain alpha‑keto acid dehydrogenase complex subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01

CAACCTGGCGACCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCGGGTTGACGGCAACGAC  >  NC_002947/4992589‑4992877
                                                                                                                                              |                                                                                                                                                  
cAACCTGGCGACCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATAcc                                                                                                                                              <  4:214421/149‑1 (MQ=255)
    cTGGCGACCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCgcgc                                                                                                                                          <  2:228181/149‑1 (MQ=255)
       gCGACCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGccc                                                                                                                                        <  6:197904/148‑1 (MQ=255)
       gCGACCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGcc                                                                                                                                         <  2:142608/147‑1 (MQ=255)
          aCCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGt                                                                                                                                    >  4:299629/1‑149 (MQ=255)
             cAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCAt                                                                                                                                 >  8:155932/1‑149 (MQ=255)
                  cGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGACCACGTATACCGCGCCCCGGTCATCCTc                                                                                                                             >  4:235164/1‑148 (MQ=255)
                        ggCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACATATACCGCGCCCCGGTCATCCTCAACGTgg                                                                                                                      >  7:126110/1‑149 (MQ=255)
                        ggCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACATATACCGCGCCCCGGTCATCCTCAACGTgg                                                                                                                      >  1:258551/1‑149 (MQ=255)
                           ggTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTca                                                                                                                   <  7:155932/149‑1 (MQ=255)
                                 cTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCTACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCaacaac                                                                                                              >  3:308869/1‑148 (MQ=255)
                                     ttgatctcATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATg                                                                                                         <  1:276248/142‑1 (MQ=255)
                                             aTCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATg                                                                                                         >  2:276248/1‑141 (MQ=255)
                                                ggCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACATATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTcc                                                                                               <  2:258551/148‑1 (MQ=255)
                                                ggCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACATATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTcc                                                                                               <  8:126110/148‑1 (MQ=255)
                                                 gCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTGCTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCAc                                                                                             >  3:194428/1‑149 (MQ=255)
                                                 gCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCAc                                                                                             <  5:199227/149‑1 (MQ=255)
                                                      cAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACATTc                                                                                         >  2:77789/1‑148 (MQ=255)
                                                        aGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAg                                                                                      <  3:299629/149‑1 (MQ=255)
                                                        aGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAg                                                                                      <  2:8023/149‑1 (MQ=255)
                                                         gggCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTcc                                                                                               >  1:136825/1‑139 (MQ=255)
                                                         gggCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTcc                                                                                               >  1:244207/1‑139 (MQ=255)
                                                         gggCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTcc                                                                                               <  2:244207/139‑1 (MQ=255)
                                                         gggCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTcc                                                                                               <  2:136825/139‑1 (MQ=255)
                                                         gggCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAgg                                                                                     <  5:319719/149‑1 (MQ=255)
                                                                   aaGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCAATCGCCgg                                                                           >  1:320453/1‑149 (MQ=255)
                                                                              ggCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCTACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGa                                                                <  4:308869/149‑1 (MQ=255)
                                                                               gCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGac                                                               >  5:111829/1‑149 (MQ=255)
                                                                               gCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGac                                                               >  5:130138/1‑149 (MQ=255)
                                                                                 aTGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTg                                                                                                                       >  3:109327/1‑91 (MQ=255)
                                                                                 aTGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTg                                                                                                                       <  4:109327/91‑1 (MQ=255)
                                                                                    gATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCt                                                          >  7:159048/1‑149 (MQ=255)
                                                                                                 gcTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCTGTCATCCTCAACGTGGTCAAAAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCcgtgg                                             >  8:291428/1‑149 (MQ=255)
                                                                                                 gcTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAAAGGGCCATTTCCACCTTCCACGCCATCGCCGGTGGCGAGTCGACCACCGTTGCCGGCcgtgg                                             >  8:287121/1‑149 (MQ=255)
                                                                                                        ccGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCcgtggcgtgag                                       >  7:246291/1‑146 (MQ=255)
                                                                                                        ccGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCcgtggcgt                                          <  8:246291/145‑1 (MQ=255)
                                                                                                        ccGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCCCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGt                                      >  3:47740/1‑149 (MQ=255)
                                                                                                           aGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGc                                   >  6:243834/1‑149 (MQ=255)
                                                                                                            gTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCTGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCg                                  <  1:77789/149‑1 (MQ=255)
                                                                                                            gTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCg                                  <  6:130138/149‑1 (MQ=255)
                                                                                                               ggACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCACCGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCgg                                 <  3:235164/147‑1 (MQ=255)
                                                                                                                  cTTCCACACCGCCCTTACCTTTGACCACGTATACCACGCACCGGTCATCCTCAACGAGGTCAACAACCAATCGGCCATTTACACCTTCCAGGCCATCGCCGGAGGAGAGTCGACAACATTTGCCGGCCGAGGCGTGGGTTGCGGTAtt                             >  5:316781/1‑148 (MQ=255)
                                                                                                                   ttCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGc                           >  8:63162/1‑149 (MQ=255)
                                                                                                                   ttCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGc                           >  2:301408/1‑149 (MQ=255)
                                                                                                                      cacaCCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCtt                         <  4:194428/148‑1 (MQ=255)
                                                                                                                         acCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCg                                  <  4:193047/136‑1 (MQ=255)
                                                                                                                         acCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCg                                  >  3:193047/1‑136 (MQ=255)
                                                                                                                         acCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCt                     >  8:130162/1‑149 (MQ=255)
                                                                                                                           cGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGGTGCGGTATTGCTTCGCTGc                   >  1:234574/1‑149 (MQ=255)
                                                                                                                            gCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCg                  <  7:130162/149‑1 (MQ=255)
                                                                                                                             cccTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCAATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCgg                 <  2:320453/149‑1 (MQ=255)
                                                                                                                                  aCCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCGGGtt              <  6:111829/147‑1 (MQ=255)
                                                                                                                                         cccACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCGGGTTGACGGCAAc     <  4:47740/149‑1 (MQ=255)
                                                                                                                                            atgTATACCGCGCCCCTGTCATCCTCAACGTTGTCAACAACCAATGGGCTATTTCCAACTTCCAGGTCATAGCCGTTGTCGAGTCGACCAACTTTTACGGCCGTGGCGTGGGTTGCGGTATTTCTTCGCTGCTGGTTGACGGGAACGAc  <  6:316781/147‑1 (MQ=255)
                                                                                                                                            aCGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCGGGTTGACGGCAACGAc  >  5:254455/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CAACCTGGCGACCCAGTTCGTGCAGGCGGTCGGCTGGGCCATGGCATCGGCGATCAAGGGCGATACCAAGATCGCCTCGGCATGGATCGGTGACGGCGCTACTGCCGAGTCGGACTTCCACACCGCCCTTACCTTTGCCCACGTATACCGCGCCCCGGTCATCCTCAACGTGGTCAACAACCAATGGGCCATTTCCACCTTCCAGGCCATCGCCGGTGGCGAGTCGACCACCTTTGCCGGCCGTGGCGTGGGTTGCGGTATTGCTTCGCTGCGGGTTGACGGCAACGAC  >  NC_002947/4992589‑4992877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑