| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,064,615 | C→T | 13.5% | A128T (GCA→ACA) ‡ | PP_4461 ← | MFS transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,064,615 | 0 | C | T | 13.5% | 146.3 / 12.3 | 59 | A128T (GCA→ACA) ‡ | PP_4461 | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base T (4/4); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
GGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTT > NC_002947/5064467‑5064762 | ggAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTgc > 2:280409/1‑149 (MQ=255) aaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTgcgg < 3:102918/149‑1 (MQ=255) aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGaa > 7:239707/1‑149 (MQ=255) aatagCGCATAGTCTACTAGCGAGGACAGCAAAGCGCCCCAATAGTAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGATGTGCCCCATTCGCCGCAGGTGGCGAAtg < 8:280618/145‑2 (MQ=255) gagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtgggg < 7:167677/149‑5 (MQ=255) gagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCtt > 4:308098/1‑149 (MQ=255) cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga < 8:239707/149‑1 (MQ=255) cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga < 4:181920/149‑1 (MQ=255) cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga < 2:26683/149‑1 (MQ=255) cGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGAc < 1:280409/149‑1 (MQ=255) ctactaGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAtctc < 6:253102/149‑1 (MQ=255) taGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACa > 3:207487/1‑149 (MQ=255) gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt < 6:138569/148‑1 (MQ=255) aGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTgcgg > 4:90763/1‑120 (MQ=255) aGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTgcgg < 3:90763/120‑1 (MQ=255) gcaaGCAAAGCGCACCCAGAGTAGCTAACGCTCTTGAAACTTCCATATAATCCCTTACGACCTGCAGGAGAACATTCCACGATATAAGATGCTGAGGTGCCCCATTCGCCAGCTGCTGCGAATCCTTGGAGACATCTCAACATGACTAg < 2:203778/146‑1 (MQ=255) gCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGaa > 3:29458/1‑122 (MQ=255) gCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGaa < 4:29458/122‑1 (MQ=255) cgcCCCCAGAGGACCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACTATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa < 1:215742/149‑1 (MQ=255) gcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAAc < 6:270264/130‑1 (MQ=255) gcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAAc > 5:270264/1‑130 (MQ=255) ccccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGAAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCa > 3:172039/1‑149 (MQ=255) cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGAc > 2:70665/1‑119 (MQ=255) cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc < 7:30583/149‑1 (MQ=255) ccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa > 6:66544/1‑107 (MQ=255) ccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa < 1:254555/107‑1 (MQ=255) ccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa < 5:66544/107‑1 (MQ=255) ccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa > 2:254555/1‑107 (MQ=255) gaggagCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAAc < 3:308098/149‑1 (MQ=255) cTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAAt < 7:176140/149‑1 (MQ=255) cTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAAt < 3:254101/149‑1 (MQ=255) aCGCTCTGGAAACTTCCAAAGAATTCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGt > 1:231203/1‑149 (MQ=255) ggAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTAt > 5:145010/1‑149 (MQ=255) aaCTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGTAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt < 4:172039/149‑1 (MQ=255) aaCTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt > 6:41430/1‑149 (MQ=255) aCTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACTATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg < 1:269969/149‑1 (MQ=255) cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCACCATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt > 7:297282/1‑148 (MQ=255) cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt > 5:321431/1‑148 (MQ=255) gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac < 4:207487/149‑1 (MQ=255) cccTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaa > 6:85889/1‑148 (MQ=255) ccTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAGTTGGAGCACCAACTCCAATAGTCTCGTATCTTTGAATCAGaccaata > 7:80781/1‑149 (MQ=255) ccTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaata < 5:41430/149‑1 (MQ=255) cTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACt > 4:94328/1‑94 (MQ=255) cTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACt < 3:94328/94‑1 (MQ=255) cTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac < 6:145010/149‑1 (MQ=255) aCGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatacca < 3:119441/148‑1 (MQ=255) cGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaataccaat < 3:152397/149‑1 (MQ=255) aCGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAAc > 6:117051/1‑89 (MQ=255) aCGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAAc < 5:117051/89‑1 (MQ=255) aCGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATc > 8:237622/1‑114 (MQ=255) aCGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATc < 7:237622/114‑1 (MQ=255) aCGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCaaa > 4:55705/1‑148 (MQ=255) cGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAaata < 3:55705/149‑1 (MQ=255) tataAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAaataat < 2:231203/148‑1 (MQ=255) gATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTAGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTcag > 2:102538/1‑149 (MQ=255) tccaaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACTCCAATAGTCTCGTATGTTGGAATAAGACCAATACCAATACTGCCGATTGCCATGAAAATAATCGTCAGCAGCATcgc > 4:254183/8‑149 (MQ=255) ccccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg > 3:156321/1‑149 (MQ=255) ggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACTAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggggt > 2:316157/3‑147 (MQ=255) ttCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaataccaat < 3:4964/102‑1 (MQ=255) ttCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaataccaat > 4:4964/1‑102 (MQ=255) ccACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGccc > 2:253993/1‑149 (MQ=255) aCCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGccccc > 8:160087/1‑149 (MQ=255) aCCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACAAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGccccc > 5:298839/1‑149 (MQ=255) cggcgAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGTAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCtttt < 6:288142/148‑1 (MQ=255) | GGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTT > NC_002947/5064467‑5064762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |