Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,065,144 A→G 8.6% L210S (TTG→TCG)  PP_4462 ← 4‑hydroxy‑4‑methyl‑2‑oxoglutarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,065,1440AG 8.6% 129.1 / 2.2 49L210S (TTG→TCG) PP_44624‑hydroxy‑4‑methyl‑2‑oxoglutarate aldolase
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base G (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTAGGGCACCGCACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGTTCTCATCGCAGATCACAATGTCGCCTGG  >  NC_002947/5065011‑5065292
                                                                                                                                     |                                                                                                                                                    
gAGTAGGGCACCGCACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTc                                                                                                                                                  <  1:100993/138‑1 (MQ=255)
gAGTAGGGCACCGCACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTc                                                                                                                                                  >  2:100993/1‑138 (MQ=255)
         aCCGCACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTtctc                                                                                                                              >  6:93981/1‑149 (MQ=255)
             cACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAAt                                                                                                                          <  7:54809/149‑1 (MQ=255)
             cACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAAt                                                                                                                          <  1:218043/149‑1 (MQ=255)
                gTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATtcg                                                                                                                       >  7:86273/1‑149 (MQ=255)
                 tAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATtcgt                                                                                                                      <  6:330688/149‑1 (MQ=255)
                    cGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGa                                                                                                                   <  4:314912/149‑1 (MQ=255)
                                     aTGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCcgcg                                                                                                  <  5:93981/149‑1 (MQ=255)
                                        agtCGTGTTTATTTTCCATCCCGCATATCAAGTCTTTAAGGGTGGGTCACTTCCATCTACATATCTGCTTCTCCACTCGCTGTAGCACCCGACAAGTCGCCGAGTTTTTCACCTTCTCTACTTCGTCGACAGTTCTCAACGCCGCGATc                                                                                               <  6:107326/147‑1 (MQ=255)
                                        aTTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATc                                                                                               >  7:117331/1‑149 (MQ=255)
                                          tCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCt                                                                                             >  6:258760/1‑149 (MQ=255)
                                             tttttATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCt                                                                                             >  1:86597/1‑146 (MQ=255)
                                             tttttATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCt                                                                                             <  2:86597/146‑1 (MQ=255)
                                               ttttttttTCATACCCCATAGCAAGGCTTGAAGGGTGGGTGAATTTCAACAACATTTATGCTTGGAAAAAAGCTGTGGCCCCCCACCAGTGGCCGCGGTTTTGCGCGTCTCTAATTGGTGGACAGTTGTGAACGCCGCGGTccgggttt                                                                                        >  2:305814/1‑143 (MQ=255)
                                                      tCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAgg                                                                                  <  4:177717/148‑1 (MQ=255)
                                                        cATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGcc                                                                               >  2:97988/1‑149 (MQ=255)
                                                           cccGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACGAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGtt                                                                            >  7:105335/1‑149 (MQ=255)
                                                           cccGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACGAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGtt                                                                            >  7:114542/1‑149 (MQ=255)
                                                             cGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCACAAACGCGACGACAGTTCTCACCGCCGCCATCCAGTTTACCCAGGGCCGttt                                                                           >  1:108377/1‑148 (MQ=255)
                                                              gCATCGCATGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTGCTACAATCGCTGGCGCACCCGACTAGTCGCCGTGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGa                                                                         <  2:108377/149‑1 (MQ=255)
                                                              gCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCt                                                                                             >  6:293504/1‑129 (MQ=255)
                                                              gCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCt                                                                                             <  5:293504/129‑1 (MQ=255)
                                                               cATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGaa                                                                        <  1:97988/149‑1 (MQ=255)
                                                                aTAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGaac                                                                       <  8:86273/149‑1 (MQ=255)
                                                                     aaGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAAcgccg                                                                                                    >  5:233167/1‑115 (MQ=255)
                                                                     aaGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAAcgccg                                                                                                    <  6:233167/115‑1 (MQ=255)
                                                                     aaGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACtt                                                                  >  8:38762/1‑149 (MQ=255)
                                                                       ggCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGttt                                                                           >  2:193842/1‑138 (MQ=255)
                                                                       ggCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGttt                                                                           <  1:193842/138‑1 (MQ=255)
                                                                         cTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATc                                                              <  5:258760/149‑1 (MQ=255)
                                                                         cTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATc                                                              <  1:61335/149‑1 (MQ=255)
                                                                             aaGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAg                                                           <  8:117331/148‑1 (MQ=255)
                                                                             aaGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGt                                                          >  1:137677/1‑149 (MQ=255)
                                                                                         aTTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTc                                              <  2:120753/149‑1 (MQ=255)
                                                                                          ttCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACt                                               >  7:59330/1‑147 (MQ=255)
                                                                                             cAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGaa                                          <  6:223894/149‑1 (MQ=255)
                                                                                                 tACATATCTGCTTCTACAATCGCTGTCGCACCCGACGAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAg                                      <  8:114542/149‑1 (MQ=255)
                                                                                                 tACATATCTGCTTCTACAATCGCTGTCGCACCCGACGAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAg                                      <  8:105335/149‑1 (MQ=255)
                                                                                                 tACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAg                                      <  1:305814/149‑1 (MQ=255)
                                                                                                 tACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAg                                      <  8:59330/149‑1 (MQ=255)
                                                                                                          gCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGt                              >  3:54065/1‑148 (MQ=255)
                                                                                                          gCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGt                              >  3:58406/1‑148 (MQ=255)
                                                                                                           cTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGtt                             <  7:38762/148‑1 (MQ=255)
                                                                                                            ttCTACAATCGCTGTCTCACCCTACAATTCGACGAGTTTTTCCCATTCTCTAATTCGTCGACAAATATAAACGAAGAGAACCTGATTATCCAGGGCCTTTTGAACAACTTCATCTAGTTACTCTGTAATCAGAAAGATGACTca                                >  2:194193/1‑143 (MQ=255)
                                                                                                                  aaTCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGTTCTCATCg                      >  1:16917/1‑148 (MQ=255)
                                                                                                                            gggtgcGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAg                                      >  4:233058/6‑122 (MQ=255)
                                                                                                                            gggtgcGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAg                                      <  3:233058/117‑1 (MQ=255)
                                                                                                                                ccGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGTTCTCATCGCAGATCACAATGTCg       >  1:208184/1‑149 (MQ=255)
                                                                                                                                     aaGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGTTCTCATCGCAGATCACAATGTCGCCTgg  <  2:137677/149‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                                    
GAGTAGGGCACCGCACGTAACGCCACCGTGCGGGGCAATGATTCGTTTTTATTTTCCATCCCGCATAGCAAGGCTTGAAGGGTGGGTCAATTCCAACTACATATCTGCTTCTACAATCGCTGTCGCACCCGACAAGTCGCCGAGTTTTTCCCCTTCTCTAATTCGTCGACAGTTCTCAACGCCGCGATCCTGTTTAGCCAGGGCCGTTTGAACAACTTCATCTAGTTCCGCTGGACTCAGAACGAGGACTCCGTTCTCATCGCAGATCACAATGTCGCCTGG  >  NC_002947/5065011‑5065292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑