Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,142,698 G→C 7.4% A345P (GCG→CCG)  PP_4526 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,142,6980GC 7.4% 171.2 / 4.1 54A345P (GCG→CCG) PP_4526MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (20/30);  new base C (2/2);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGTTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGAGCCGG  >  NC_002947/5142550‑5142843
                                                                                                                                                     |                                                                                                                                                  
tgtTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTggcgg                                                                                                                                                    >  6:152148/1‑149 (MQ=255)
  ttGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTggcggc                                                                                                                                                   <  2:150322/148‑1 (MQ=255)
  ttGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGgcggcg                                                                                                                                                  <  4:270243/149‑1 (MQ=255)
  ttGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGgcggcg                                                                                                                                                  <  4:265753/149‑1 (MQ=255)
    gcgcTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCt                                                                                                                                                >  7:117029/1‑149 (MQ=255)
     cgcTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCt                                                                                                                                                >  7:267002/1‑148 (MQ=255)
      gcTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTca                                                                                                                                              <  2:293396/149‑1 (MQ=255)
       cTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTcag                                                                                                                                             <  5:248438/149‑1 (MQ=255)
         gtgtgGCGGGCCGTTCCTGGCCTGCAGGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGTCAGTTGAGTGGGCCGCTGCTGGCGGCGCTcagca                                                                                                                                           <  1:205473/149‑1 (MQ=255)
          tgtgGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTcagca                                                                                                                                           <  8:39729/148‑1 (MQ=255)
          tgtgGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTcagca                                                                                                                                           <  2:245898/148‑1 (MQ=255)
           gtgGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGCCagcagc                                                                                                                                         >  5:249787/1‑149 (MQ=255)
              gCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGCCagcagcgg                                                                                                                                       <  6:249787/148‑3 (MQ=255)
                   ccGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAg                                                                                                                                 >  5:218147/1‑149 (MQ=255)
                   ccGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAg                                                                                                                                 >  3:219464/1‑149 (MQ=255)
                    cGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGCCagcagcgg                                                                                                                                       >  3:62861/1‑140 (MQ=255)
                    cGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGCCagcagcgg                                                                                                                                       <  4:62861/142‑3 (MQ=255)
                    cGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGc                                                                                                                                <  8:117029/149‑1 (MQ=255)
                             ccTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTg                                                                                                                      <  4:147188/149‑1 (MQ=255)
                              cTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTg                                                                                                                      >  2:307050/1‑148 (MQ=255)
                                      aaCTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTg                                                                                                                      >  5:217366/1‑140 (MQ=255)
                                      aaCTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTg                                                                                                                      <  6:217366/140‑1 (MQ=255)
                                       aCTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCAt                                                                                                            >  4:97890/1‑149 (MQ=255)
                                        cTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCAtt                                                                                                           <  3:245963/149‑1 (MQ=255)
                                         tGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCAtt                                                                                                           >  8:77232/1‑148 (MQ=255)
                                           gTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTg                                                                                                          <  1:199862/147‑1 (MQ=255)
                                           gTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTg                                                                                                          <  8:267002/147‑1 (MQ=255)
                                           gTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGAt                                                                                                        >  7:115335/1‑149 (MQ=255)
                                                         gcgcgAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCggcgggcg                                                                                          >  6:61248/1‑149 (MQ=255)
                                                               gTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtgg                                                                                    <  2:43499/149‑1 (MQ=255)
                                                                  ggCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCAGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                                 >  2:262564/1‑149 (MQ=255)
                                                                   gcgcCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGCCTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                                 <  1:228784/149‑1 (MQ=255)
                                                                   gcgcCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                                 <  4:305824/148‑1 (MQ=255)
                                                                   gcgcCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                                 <  6:218147/148‑1 (MQ=255)
                                                                    cgcCTCAGGCCACACAGCGCAATGCCGGGCTGCTGCCCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtggtg                                                                               <  7:220161/149‑1 (MQ=255)
                                                                       cacaGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGtggct                                                                            <  7:77232/149‑1 (MQ=255)
                                                                             ccACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCggg                                                                      >  8:152554/1‑149 (MQ=255)
                                                                                cacaGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtggt                                                                                >  7:108489/1‑136 (MQ=255)
                                                                                     cgcAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCa                                                              <  8:115335/149‑1 (MQ=255)
                                                                                      gcAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAg                                                             <  3:290870/149‑1 (MQ=255)
                                                                                       cAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGt                                                            >  4:2508/1‑149 (MQ=255)
                                                                                           gCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGcc                                                        >  6:155731/1‑149 (MQ=255)
                                                                                                 ctgctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGa                                                  >  3:101223/1‑149 (MQ=255)
                                                                                                   gctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATg                                                >  8:216118/1‑149 (MQ=255)
                                                                                                   gctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATg                                                >  3:153645/1‑149 (MQ=255)
                                                                                                    ctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATg                                                <  1:307050/148‑1 (MQ=255)
                                                                                                       aCCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGcac                                             <  5:61248/148‑1 (MQ=255)
                                                                                                        ccGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGcgggcggg                                                                                        <  2:250715/104‑1 (MQ=255)
                                                                                                        ccGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGcgggcggg                                                                                        >  1:250715/1‑104 (MQ=255)
                                                                                                          gCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCacaaca                                         <  5:152148/149‑1 (MQ=255)
                                                                                                                    ccTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAgcgc                                <  5:155731/148‑1 (MQ=255)
                                                                                                                        ggggCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGGGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGt                            >  1:101343/1‑148 (MQ=255)
                                                                                                                        ggggCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGt                            <  4:101223/148‑1 (MQ=255)
                                                                                                                                 gAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAg                   <  7:152554/148‑1 (MQ=255)
                                                                                                                                  aGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCTGTTGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGTGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGTGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGt                 <  3:122124/149‑1 (MQ=255)
                                                                                                                                    tGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCCCATTGATGCTGGCGGCGGGCGGGTTGGGGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGtt                <  1:262564/148‑1 (MQ=255)
                                                                                                                                      ggCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTGCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCa             <  3:97890/149‑1 (MQ=255)
                                                                                                                                            tgctgGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGa       <  3:2508/149‑1 (MQ=255)
                                                                                                                                                  gcggcgCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGAGCCgg  <  7:216118/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGTTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGGCGGCGCTCAGCAGCCACTACAGCGGTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGAGCCGG  >  NC_002947/5142550‑5142843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: