Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,162,210	T→A	6.6%	A326A (GCT→GCA) ‡	PP_4544 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,162,210	0	T	A	6.6%	206.1 / 2.5	70	A326A (GCT→GCA) ‡	PP_4544	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/33);  new base A (3/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTAC  >  NC_002947/5162069‑5162354                                                                                                                                              |                                                                                                                                                  cGGCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTg                                                                                                                                             <  4:9679/148‑1 (MQ=255) cGGCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCAGCCAGCCAGGCCTg                                                                                                                                             <  7:148153/148‑1 (MQ=255)   gCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaa                                                                                                                                           >  8:36401/1‑148 (MQ=255)    cAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaaaa                                                                                                                                         >  1:243121/1‑149 (MQ=255)    cAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCAGCCAGCCAGGCCTGatct                                                                                                                                         <  7:145391/149‑4 (MQ=255)     aaCACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAAc                                                                                                                                        <  7:30639/149‑1 (MQ=255)     aaCACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAAc                                                                                                                                        <  2:243121/149‑1 (MQ=255)        accaccAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCaa                                                                                                                                     <  1:205260/149‑1 (MQ=255)             caACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATgg                                                                                                                                >  8:75180/1‑149 (MQ=255)             caACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGATGGCCTGAAAACCAAAATgg                                                                                                                                >  4:250116/1‑149 (MQ=255)                  aGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcc                                                                                                                           <  6:189605/149‑1 (MQ=255)                   gTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct                                                                                                                          >  3:25434/1‑149 (MQ=255)                       aaTCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct‑ggag                                                                                                                     >  3:142410/1‑149 (MQ=255)                         tCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct‑ggagcc                                                                                                                   >  3:120345/1‑149 (MQ=255)                             gtggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct‑ggagcctgga                                                                                                               <  8:69898/149‑1 (MQ=255)                             gtggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct‑ggagcctg                                                                                                                 <  8:92269/147‑1 (MQ=255)                             gtggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct‑ggagcctg                                                                                                                 <  7:36401/147‑1 (MQ=255)                                gAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACt                                                                                                             <  7:304392/148‑1 (MQ=255)                                         ggCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACcg                                                                                                   >  8:221252/1‑149 (MQ=255)                                          gCCAGATCGACTTCACTGGCTTGTCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCAACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACACCTACcgc                                                                                                  >  6:27248/1‑149 (MQ=255)                                          gCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaaa                                                                                                                                          <  1:70200/110‑1 (MQ=255)                                          gCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaaa                                                                                                                                          >  2:70200/1‑110 (MQ=255)                                          gCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACcgc                                                                                                  >  7:150356/1‑149 (MQ=255)                                                tCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGAtgtg                                                                                            <  7:75180/149‑1 (MQ=255)                                                  gACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGAtgtg                                                                                            >  6:169376/1‑147 (MQ=255)                                                  gACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCa                                                                                          <  4:25434/149‑1 (MQ=255)                                                       cACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGt                                                                                      <  5:169376/148‑1 (MQ=255)                                                        aCTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTaa                                                                                    >  8:247288/1‑149 (MQ=255)                                                         cTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACAGACGGCGAAGATCACGCCTCGAAGGCAGGCCTCAAAACAAAAATGCCTGCCT‑GGATCCTGCACAACAACTACCGCCATGTGCAGCGAAAc                                                                                   >  6:209656/1‑149 (MQ=255)                                                            gCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAAcgcg                                                                                >  8:290774/1‑149 (MQ=255)                                                              ttGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggt                                                                              >  2:259780/1‑149 (MQ=255)                                                                ggCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                             <  3:250116/148‑1 (MQ=255)                                                                ggCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                             <  1:21381/148‑1 (MQ=255)                                                                   cATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGt                                                                                      >  3:268198/1‑136 (MQ=255)                                                                   cATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGt                                                                                      <  4:268198/136‑1 (MQ=255)                                                                     ttCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGc                                                                       >  2:211833/1‑149 (MQ=255)                                                                     ttCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCAACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGc                                                                       <  5:27248/149‑1 (MQ=255)                                                                       ccGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGcc                                                                      <  4:90539/148‑1 (MQ=255)                                                                             tCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCa                                                                <  1:259780/148‑1 (MQ=255)                                                                                  cTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGCtt                                                          >  1:98731/1‑149 (MQ=255)                                                                                       gCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCATCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGAc                                                     >  3:194343/1‑149 (MQ=255)                                                                                       gCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGAc                                                     <  1:211833/149‑1 (MQ=255)                                                                                        ccAGTACGTGAAGAACACCGACAGCACCGAGGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACt                                                    >  1:27592/1‑149 (MQ=255)                                                                                           gTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCg                                                 <  7:247288/149‑1 (MQ=255)                                                                                           gTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCg                                                 <  1:123267/149‑1 (MQ=255)                                                                                            tACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCgg                                                >  2:115953/1‑149 (MQ=255)                                                                                             aCGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCgg                                                >  7:128260/1‑148 (MQ=255)                                                                                                tGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAAc                                            >  5:114469/1‑149 (MQ=255)                                                                                                 gaagaaCACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                             >  8:34143/1‑115 (MQ=255)                                                                                                 gaagaaCACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                             <  7:34143/115‑1 (MQ=255)                                                                                                       cacCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGAGGTGGTCAGCCTGTTCACCGATTCGTACTTCGGCAACGGCTaca                                     <  2:98731/149‑1 (MQ=255)                                                                                                         ccGACAGCAACGAAGGCGAAGATCAGGCCTGGCTGGCTGTCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGAAGCGTAACTCGGTGGTCAGCCTGTTAACATATTCGGACTTCGTCAAAGGCTACAcc                                   >  4:92545/1‑149 (MQ=255)                                                                                                            aCAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACg                                           <  3:157601/138‑1 (MQ=255)                                                                                                            aCAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACg                                           >  4:157601/1‑138 (MQ=255)                                                                                                                cACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCgg                                                <  2:308923/129‑1 (MQ=255)                                                                                                                cACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCgg                                                >  1:308923/1‑129 (MQ=255)                                                                                                                   cGACGGCGAAGATCAGGGCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTcgcgcg                         <  2:102812/149‑1 (MQ=255)                                                                                                                   cGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTcgcgcg                         <  8:128260/149‑1 (MQ=255)                                                                                                                        gCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTcgcgcg                         >  6:169399/1‑144 (MQ=255)                                                                                                                        gCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTcgcgcg                         <  5:169399/144‑1 (MQ=255)                                                                                                                          gAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTcac                    <  1:115953/147‑1 (MQ=255)                                                                                                                          gAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTcac                    >  1:9901/1‑147 (MQ=255)                                                                                                                          gAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACaa                  <  7:221252/149‑1 (MQ=255)                                                                                                                          gAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACaa                  >  1:182808/1‑149 (MQ=255)                                                                                                                            ttttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGt                >  1:68310/4‑149 (MQ=255)                                                                                                                            aGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGt                <  8:150356/149‑1 (MQ=255)                                                                                                                               tCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTACCGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTc              >  8:117839/1‑148 (MQ=255)                                                                                                                                cAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGt                <  4:178882/145‑1 (MQ=255)                                                                                                                                cAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGt                >  3:178882/1‑145 (MQ=255)                                                                                                                                   gCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGt         >  8:172310/1‑149 (MQ=255)                                                                                                                                            gctggcCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTAc  <  4:142410/147‑1 (MQ=255)                                                                                                                                            gctggcCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTAc  >  3:64074/1‑147 (MQ=255)                                                                                                                                              tggcCTGAAAACCAAAATGGGTGCCTGGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTAc  >  1:14986/1‑146 (MQ=255)                                                                                                                                              |                                                                                                                                                  CGGCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCT‑GGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTAC  >  NC_002947/5162069‑5162354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑