| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,162,622 | C→G | 12.4% | intergenic (+145/+56) | PP_4544 → / ← PP_4545 | hypothetical protein/beta‑ketodecanoyl‑ACP synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,162,622 | 0 | C | G | 12.4% | 88.6 / 2.9 | 32 | intergenic (+145/+56) | PP_4544/PP_4545 | hypothetical protein/beta‑ketodecanoyl‑ACP synthase |
| Reads supporting (aligned to +/- strand): ref base C (10/18); new base G (2/2); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTG > NC_002947/5162477‑5162767 | aGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGc < 8:52995/146‑1 (MQ=255)aGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCt < 5:141539/147‑1 (MQ=255) cgctgctTCACCTGACGGGGTAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGGTACAGCACAGTTCTCAAGGTCTGTGGTGATACTGTGGGAGc < 3:163325/147‑1 (MQ=17) tgctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTa < 5:253738/135‑1 (MQ=255) tgctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCg < 6:110210/147‑1 (MQ=255) ctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTACGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCgg < 8:36291/146‑1 (MQ=255) ccTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGccc < 4:89058/149‑1 (MQ=255) cTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTAc < 1:194564/128‑1 (MQ=255) acgCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCcgc < 1:116121/145‑1 (MQ=255) aGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCTTGCCcgctcc < 8:157056/149‑4 (MQ=255) ggggtaaaCCCCGACCCCATCGGGGGTTTCCGGCTTCTTTTTGTCTGTGTTAAATGCGCCGGCCCCTTCGCGGGCTTGCCGGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAag < 6:185946/144‑1 (MQ=37) gACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCCCTCCCACAGTATCACCCCAGACCATGAGAACTGTGGTGTACCGGTGGGGGCGGGCAAGCCCGCGAAGAGGCCGTAAAAg > 3:267660/1‑149 (MQ=38) ccGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTgg < 2:221229/99‑1 (MQ=255) ccGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTgg > 1:221229/1‑99 (MQ=255) cgcCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc < 5:66396/148‑1 (MQ=255) cTTGCCCGCTCCCACAGTATCACCAAAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACg < 4:267660/149‑1 (MQ=255) ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAAc > 1:129610/1‑147 (MQ=255) gCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAg < 2:129610/148‑1 (MQ=255) cccGCTCCCACAGGTACAGCACAGTTCTCAAGGTCTGTGGTGATACTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGa < 6:108560/146‑1 (MQ=17) cccGCTCCCACAGGTACAGCACAGTTCTCAAGGTCTGTGGTGATACTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACAAAACGa > 5:108560/1‑146 (MQ=17) ccGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCt > 1:123260/1‑148 (MQ=255) cGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTc > 3:56906/1‑148 (MQ=255) ccacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGCGCTCAACACGCCCAACGAGcc > 5:322856/1‑149 (MQ=255) cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCt > 4:198364/1‑149 (MQ=255) caGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCttt > 6:52671/1‑149 (MQ=255) gagaACTGTGCTGTACCTGTGGTAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAg < 8:221755/147‑1 (MQ=255) aCTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATc < 4:56906/149‑1 (MQ=255) cTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATcc < 5:52671/149‑1 (MQ=255) cTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATcc > 2:305398/1‑149 (MQ=255) cTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATcc > 2:300615/1‑149 (MQ=255) tgtgCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCt > 7:294045/1‑149 (MQ=255) tgCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTg < 1:305398/148‑1 (MQ=255) tgCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTg < 1:300615/148‑1 (MQ=255) | AGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTG > NC_002947/5162477‑5162767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |