| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 396,855 | G→T | 6.3% | R31R (CGG→AGG) | PP_0329 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 396,855 | 0 | G | T | 6.3% | 205.3 / 3.4 | 63 | R31R (CGG→AGG) | PP_0329 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (31/28); new base T (2/2); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
AAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTA > NC_002947/396715‑396999 | aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt > 2:215475/1‑148 (MQ=255) aGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑Gaa > 6:283884/1‑149 (MQ=255) gtgtGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATc < 4:115861/149‑1 (MQ=255) gATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGtt > 4:199858/1‑148 (MQ=255) aTTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGttt < 4:185646/148‑1 (MQ=255) ccGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGCAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTgg > 3:215516/1‑148 (MQ=255) cGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGt < 1:163652/148‑1 (MQ=255) gTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGt > 2:221384/1‑147 (MQ=255) cGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGc < 5:283884/149‑1 (MQ=255) tGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctg < 1:215475/149‑1 (MQ=255) cTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCGGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggt > 1:245638/1‑149 (MQ=255) aCGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa > 2:2504/1‑136 (MQ=255) gACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGtt > 2:290517/1‑117 (MQ=255) aaGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAg > 8:2230/1‑148 (MQ=255) gCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGaaa > 4:287943/1‑149 (MQ=255) gTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAATAGTGAGCGTTCCAATCCGGAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgg < 4:311496/149‑1 (MQ=255) catctcatctGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgggg < 5:149343/148‑1 (MQ=255) ctcatctGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGt < 3:222419/118‑1 (MQ=255) ctcatctGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGt > 4:222419/1‑118 (MQ=255) ctGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa > 2:201283/1‑111 (MQ=255) ctGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa < 1:201283/111‑1 (MQ=255) ttGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAg < 6:215126/125‑1 (MQ=255) ttGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAg > 5:215126/1‑125 (MQ=255) ttGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGa < 3:199858/146‑1 (MQ=255) ttGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTg < 7:2230/149‑1 (MQ=255) ttGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTg > 8:251703/1‑149 (MQ=255) aaaGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAg > 2:174440/1‑138 (MQ=255) aaaGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAg < 1:174440/138‑1 (MQ=255) aaGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCa < 3:287943/149‑1 (MQ=255) tGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctg < 7:97986/105‑1 (MQ=255) tGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctg > 8:97986/1‑105 (MQ=255) ggAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGAc < 1:221384/148‑1 (MQ=255) ggAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGAc < 7:251703/148‑1 (MQ=255) aaCTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTg > 3:268545/1‑149 (MQ=255) ttCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGc > 8:204146/1‑96 (MQ=255) ttCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGc < 7:204146/96‑1 (MQ=255) ccTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGaa < 2:293238/107‑1 (MQ=255) ccTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGaa > 1:293238/1‑107 (MQ=255) ccTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCAGCAGGATT‑GACCGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCCCCGACGTGCTccc > 6:261886/1‑149 (MQ=255) tGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGt > 5:187968/1‑149 (MQ=255) tGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGGCAGTTTGGTGGTCTGGTAGAAATATTTGGGGGTATCAGGAATGTTTTTCACCGCCGTGGTcaggt > 4:20511/1‑145 (MQ=255) cttcttTGCCATCGGACTCAGCAAGAGGGATCTTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTTGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt < 6:187968/148‑1 (MQ=255) cttcttTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCATTCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt < 6:81518/148‑1 (MQ=255) tGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctg < 6:108503/91‑1 (MQ=255) tGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctg > 5:108503/1‑91 (MQ=255) ccAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTggg > 2:309490/1‑104 (MQ=255) ccAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTggg < 1:309490/104‑1 (MQ=255) cAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGtt < 4:215516/149‑1 (MQ=255) gCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTgg > 3:77929/1‑149 (MQ=255) gCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGGTCCCGTTGTCTGCAGTTGGCCCCGGaaa > 1:280136/1‑149 (MQ=255) gCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaaa < 5:68077/149‑1 (MQ=255) cAAGATGGAGCGTTCAAATCCGCATGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTg < 7:125434/132‑1 (MQ=255) cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTATGGTATAAATATTTGTGGGTATCAGTACTGTTTTTCACCGACGTTCTCCCGTTGTCTg > 8:125434/1‑132 (MQ=255) gagGGATCGTTCCAATCCGCCGGATT‑GAACGCTTCTGATCGTTTCCAATGCCAGTGGTCAGTTTGCTGGTCTGGTTGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTTTATGCAGTTGGCCCCGGAAATAGa < 7:48810/149‑1 (MQ=255) cagaaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCa < 3:297252/103‑1 (MQ=37) cagaaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCa > 4:297252/5‑107 (MQ=37) ggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATgg > 5:16322/1‑149 (MQ=255) ggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATgg > 6:196248/1‑149 (MQ=255) aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTgg > 3:280657/2‑132 (MQ=255) aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTgg < 4:280657/131‑1 (MQ=255) gTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCAGCAGTTGGCCCCGCAAATAGATGGCCTgg > 7:289732/1‑149 (MQ=255) ccAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACAGACGTGCTCCAGTTGTCTGCAGTTGGCCCCTGAAATAGATGGCATGGGc > 2:216847/1‑148 (MQ=255) aTCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGTGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTa < 2:245638/149‑1 (MQ=255) | AAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTA > NC_002947/396715‑396999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |